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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: poopy mcpooperson]
    #19046997 - 10/28/13 04:35 PM (10 years, 3 months ago)

....and then put the lids back in the plastic container? Sound s like a mess.

Just use one syringe and do 1 or 2 plates. Transfer good myc to a new plate and toss the rest.

In some ways, I'm a sheep on a bandwagon. I do things that I know work for most people most of the time.

I'm willing to help you through a process I'm familiar with, but at this point, you're kind of on your own.

Take pics, post results, good luck.


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Offlineilluminati
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: poopy mcpooperson]
    #19047016 - 10/28/13 04:36 PM (10 years, 3 months ago)

Quote:

poopy mcpooperson said:
shit. that was my worry. they dont stink though. do you even think that the creeper and TYC are contaminated? they seem to have myc growth.

i dont think im gonna throw them out just yet as i might have a good day to day pictorial of what not to do if they are contaminated.

if i did the grain agar transfer would you suggest i do them in pint jars instead of quart jars to colonize? would i have to wait for the whole jar to colonize or just enough for me to pluck a grain easily to move to agar?

i am going to use franks grain to agar TEK and dunk the grain because it seems to have a much more rapid growth.





You definitely could do them in pint jars if you want, but personally, I'd rather be left with a healthy quart of spawn in addition to getting a few plates inoculated.  Either way, wait for it to fully colonize before opening it. 

Frank definitely knows a hell of a lot more about this hobby than I do, so I'd think you'd be just fine following one of his teks.


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I didn't get turned on I just got turned
I wasn't as aroused as I was concerned
for each one of 'em I've hurt
and every time I've been burned
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Invisiblepoopy mcpooperson
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: illuminati]
    #19066753 - 10/31/13 10:37 PM (10 years, 3 months ago)

so i think i can isolate these dishes for transfer, right?

these were the only dishes that i kept.  can anybody let me know which areas to cut out? i have watched the agar isolate section from my RR videos and i have viewed a few old threads to try to figure it out and im still confused. can someone who has experience let me know if i am on the right track or not?

also, if i do isolate and the rest of the wedge is not contaminated would it be ok to drop it in a jar of grain or WBS?

DISH 1: im thinking bottom right at 8 o'clock


DISH 2: im thinking bottom right dish at 1 and 3 o'clock



DISH 3: i dont even know if this is mushroom myc. maybe bottom left 8 o'clock.


DISH 4: i was thinking 2 o'clock top right and 4 o'clock bottom right


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Invisiblepoopy mcpooperson
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: poopy mcpooperson]
    #19069932 - 11/01/13 01:29 PM (10 years, 3 months ago)

any thoughts on which sections to isolate with these dishes?


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Invisiblepoopy mcpooperson
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: poopy mcpooperson]
    #19074286 - 11/02/13 09:41 AM (10 years, 3 months ago)

can anyone tell me if where im guessing to sector for an isolate is correct?

also, if i do isolate and the rest of the wedge is not contaminated would it be ok to drop it in a jar of grain or WBS?


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InvisiblePirateSwazey
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: grainbrain]
    #19074674 - 11/02/13 11:23 AM (10 years, 3 months ago)

Quote:

grainbrain said:
Toss everything but the Creeper.  Sorry, but those other plates are beyond saving.




:foshizzle:

plates 1 & 2 look good to me, i'd wait and see whats happening with 3. 4 looks like its turning green in the bottom left. there's plenty of sectoring to take transfers from on plates 1 & 2, you've got the right idea. as for your 2nd question about inoculating a grain jar with leftover growth i wouldn't see why it would hurt, but if it was me i would just wait. before too much longer you're going to be inoculating a bunch of jars testing out separate isolations to see which one is best.

you're on the right track dude just hang in there! i wish i had started out working with agar instead of waiting so long and having so many crappy grows from chance genetics. i've been at it for a little over a year and am just picking up the agar work. before you know it you'll have a beautiful canopy of brown hats and so many mushrooms.. they'll be falling out of your ass.


Edited by PirateSwazey (11/02/13 11:52 AM)


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Invisiblepoopy mcpooperson
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: PirateSwazey]
    #19074785 - 11/02/13 11:53 AM (10 years, 3 months ago)

thanks so much.

i am trying to isolate and want to get clean, strong, and wonderfully producing cultures to work with.

lets say i use dish 2, which you said would be ok to isolate from. there are 4 separate agar sections on the petri. i think the bottom right of that dish looks the best to isolate from. i was just wondering if i could take the left over myc in that section, after i take out my pieces for isolation, and drop the entire leftover section it in some grain and if it would perform.

while im isolating i would like to see if i could get some type of growth, as small as it may be, so i can essentially practice my method of spawning/fruiting before i get my isolates where is want them.


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InvisiblePirateSwazey
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: poopy mcpooperson]
    #19075049 - 11/02/13 01:02 PM (10 years, 3 months ago)

Quote:

poopy mcpooperson said:
lets say i use dish 2, which you said would be ok to isolate from. there are 4 separate agar sections on the petri. i think the bottom right of that dish looks the best to isolate from. i was just wondering if i could take the left over myc in that section, after i take out my pieces for isolation, and drop the entire leftover section it in some grain and if it would perform.




i would pull from dish 1 and 2. theres a small section on dish 3 in the bottom left section that looks promising if you'll give it a few more days to grow out. both sides on the bottom of dish 1 have a few good sectors to transfer from. dish 2 i can see 3 good places on the bottom right section.

just remember that once you have each of these sectors isolated they will all be different substrains, and will colonize, fruit, and preform differently. some of them may preform really well and others may be total crap that you don't even want to see all the way through colonization.

the reason i am just saying to wait until you've got isolated genetics to inoculate a jar is that you're going to have so many different substrains to work with, and its only going to be another week or 2 down the road. with that time frame, if you were to inoculate a jar today, you'll be looking at 1-3 weeks of colonization time, then a g2g transfer to make up enough jars to create a monotub, which is going to take another week or 2 to colonize.

i suppose you could noc up the quart and spawn it into a little shoe box bin with 1 or 2 quarts of substrate and put that into a SGFC when its time to fruit it. if i was in your situation and dying to see some fruit bodies that is what i would probably do.

:goodluck:


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Invisiblepoopy mcpooperson
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: PirateSwazey]
    #19075075 - 11/02/13 01:08 PM (10 years, 3 months ago)

thank you very much. i thought those might some viable sections to take. i appreciate your advice.

since i first posted those photos dish 3 has grown out and could possible be the best isolate out of all of them.

have a good day.


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Offlineshaneh79
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: Violet]
    #19228238 - 12/04/13 07:00 PM (10 years, 2 months ago)

Hey Violet I am currently trying my first time isolating with agar and although its easy usiong a loop from a spore print, I'm forced to start with a syringe also. So what tek do you use to inoc grains petri dish style?  I'd appreciate any ideas..love your grows btw :smile:

Shane


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OfflinePocketRevolution
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Re: agar, ohh how i think im going to love to hate you. agar isolate log. (PICS) [Re: shaneh79]
    #19229140 - 12/04/13 09:59 PM (10 years, 2 months ago)

I like the look of dish 1 the best.  I would take samples from the bottom two sections, and transfer them to new agar plates.  Perhaps you could transfer 4 samples from each compartment to the 4 compartments of  new dishes.  Then observe the results.  The stuff along the bottom looks pretty good.

When I first looked at the pictures I thought the condensation on the lids of those plates was yeast or bacteria on the agar itself.  Good thing some more attentive members spotted the few good dishes.  You could still get some decent samples out of that.


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