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Offlineflipsidetrue
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Petri contams? Updated 11/06/13
    #19054643 - 10/29/13 10:20 PM (10 years, 3 months ago)

is it normal for mycelium to look this hairy and is it worth using?




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Edited by flipsidetrue (11/06/13 12:43 PM)


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OfflineEveniftheskyfalls
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Re: hairy mycelium? [Re: flipsidetrue]
    #19054676 - 10/29/13 10:24 PM (10 years, 3 months ago)

That looks like cobweb to me, but im a noob so dont take it to heart


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OfflineStupendous-Yappi
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Re: hairy mycelium? [Re: flipsidetrue]
    #19054682 - 10/29/13 10:26 PM (10 years, 3 months ago)

It looks like the pictures of cobweb mold I've seen. How long did it take to grow that much? Cobweb will grow a lot faster and could take over a petri dish in a few days.


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OfflineGiggle_Grower
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Re: hairy mycelium? [Re: Stupendous-Yappi]
    #19054761 - 10/29/13 10:39 PM (10 years, 3 months ago)

Looks like cobweb to me as well. Tough break.


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Offlinedodgem
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Re: hairy mycelium? [Re: Giggle_Grower]
    #19054788 - 10/29/13 10:44 PM (10 years, 3 months ago)

Looks like you are performing a recrystalization.. And mycelium should not look like a recrystalization ha.


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OfflineJanethTheGreat
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Re: hairy mycelium? [Re: flipsidetrue]
    #19054840 - 10/29/13 10:55 PM (10 years, 3 months ago)

hm.  I'm a bit of a noob, but i've had a run-in with cobweb...  it didn't get that tall nor was it that white.  Luckily cobweb is the only contam you can really take control of.  Spray some peroxide on it.  If it melts, it's cobweb and keep spraying regularly throughout the day until it's gone...  If not, it's just the branchy kind of mycelium.


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InvisiblePsiLisaBin
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Re: hairy mycelium? [Re: flipsidetrue]
    #19055267 - 10/30/13 12:07 AM (10 years, 3 months ago)

This is 100% definitely cobweb, and no, it is not worth using :sad:

garbage time!


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InvisiblePirateSwazey
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Re: hairy mycelium? [Re: PsiLisaBin]
    #19056617 - 10/30/13 08:58 AM (10 years, 3 months ago)

dat mold:cookiemonster:


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InvisiblebodhisattaMDiscordReddit
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Re: hairy mycelium? [Re: PirateSwazey]
    #19057019 - 10/30/13 11:03 AM (10 years, 3 months ago)



Looks like cobweb to me.

Here's some cobweb I cultivated for reference.


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Offlineflipsidetrue
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Re: hairy mycelium? [Re: bodhisatta]
    #19060354 - 10/30/13 09:22 PM (10 years, 3 months ago)

thanks guys i needed some back up opinions you all know how it is

thanks for the reference bodhisatta.

i went ahead and threw some fresh tissue on some agar, its been almost 48 hours since and i'm already seeing signs of growth. :rockon:


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Re: hairy mycelium? [Re: flipsidetrue]
    #19062315 - 10/31/13 09:06 AM (10 years, 3 months ago)

:thumbup:


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Offlineflipsidetrue
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Re: hairy mycelium? [Re: PirateSwazey]
    #19066612 - 10/31/13 10:18 PM (10 years, 3 months ago)

so the fresh tissue i threw on some agar hoping for some decent mycelium is looking like cobweb mold also dammit!


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Edited by flipsidetrue (10/31/13 10:38 PM)


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InvisiblePsiLisaBin
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Re: hairy mycelium? [Re: flipsidetrue]
    #19066683 - 10/31/13 10:26 PM (10 years, 3 months ago)

are you cloning a fruit and putting it on agar?
if so, was there any cobweb that you noticed present on your substrate at any time?

if I were you I would clean and lysol-bomb the hell out of the room you're working in, wash out your still air box (if you're using that and not a flowhood) with hot soapy water, and either PC your scalpel to sterilize it or replace your scalpel altogether.  (that happened to me, i had a few dishes in a row get mold and my dishes have historically always been clean - i replaced the scalpel i was using with a new one and the mold hasn't returned)  so just throwing that out there for ideas to try


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Offlineflipsidetrue
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Re: hairy mycelium? [Re: PsiLisaBin]
    #19066694 - 10/31/13 10:29 PM (10 years, 3 months ago)



I am using a still air box and not a flow hood, and i am trying to clone from a fruit that i grew outside because the cake i was doing had green mold on it. 4 months ago i got some spores from a vendor i was doing the pf tek and i got some fruits off of it, then i made some spore sprints and kept making syringes, and kept using them to knock up some more until i moved to grains, then when i moved to grains all the cakes i kept doing all got contaminated so i threw em outside still got a decent flush, but i made spore prints from those also and kept knocking up wbs jars, thinking everything was fine but the cakes just kept getting contamed  and i kept throwing them outside until it got to a point to where i only got a few fruits from it so i used those few fruits i got to try and clone and here's where im at now im sure there had to be plenty of cobweb mold associated with those and plenty of other contams


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Edited by flipsidetrue (10/31/13 10:36 PM)


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InvisibleMUSH HEAD420
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Re: hairy mycelium? [Re: flipsidetrue]
    #19066785 - 10/31/13 10:41 PM (10 years, 3 months ago)

Man it must be in the air or on your samples.

Cobweb is rare and I suppose you are dealing with the same cobweb spores from before.

You could spray your sample with some h202


Edited by MUSH HEAD420 (10/31/13 10:42 PM)


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InvisiblePsiLisaBin
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Re: hairy mycelium? [Re: MUSH HEAD420]
    #19066876 - 10/31/13 10:56 PM (10 years, 3 months ago)

ah, ok.

i do know it's hard to get a clean print off a mushroom from the great outdoors.  am assuming it is also hard to get a piece of clean tissue from a mushroom grown outdoors as well :wink:

i know you're taking it from inside the stem, so it should be relatively more protected, but the whole fruit itself is going to be "dirtier" since it's been outside versus one from inside your indoor fc.  from the looks of it, you may just not be able to get a clean sample from your outdoor cake fruits.  if you could get even a little mushroom myc going on your dish, you could transfer out away from the contam, but cobweb takes over so fast this may well be impossible to accomplish here.  i'd love to see someone more experienced with outdoor growing chime in here - if it were me, i'd probably scrap this particular endeavor and start again with an entirely new thing (clean spore syringe to agar).


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InvisibleMUSH HEAD420
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Re: hairy mycelium? [Re: PsiLisaBin]
    #19066933 - 10/31/13 11:05 PM (10 years, 3 months ago)

He would use a spore syringe if he wanted, I think he wants to try and isolate characteristics from the wild specimen.

The best way imo would be to use h2o2 to combat the cobweb mold, then transfer at least once.

There will be a chance of cobweb ending up on your cakes or grains though.


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Offlineflipsidetrue
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Re: hairy mycelium? [Re: MUSH HEAD420]
    #19067005 - 10/31/13 11:20 PM (10 years, 3 months ago)

at first i did use a spore syringe on some agar and i had the same problem. I took some advice from someone that said instead of using a spore syringe to just use actual tissue and clone



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InvisibleMUSH HEAD420
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Re: hairy mycelium? [Re: flipsidetrue]
    #19067012 - 10/31/13 11:21 PM (10 years, 3 months ago)

where the fudge is it coming from?!?


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Offlineflipsidetrue
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Re: hairy mycelium? [Re: flipsidetrue]
    #19067016 - 10/31/13 11:22 PM (10 years, 3 months ago)

i really didn't want to order more spores because to do so consists of me having to get a p.o box in another state and picking them up from there :mad2:


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