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Doost
Stranger
Registered: 10/24/13
Posts: 6
Last seen: 9 years, 4 months
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Is it done for?
#19064672 - 10/31/13 05:07 PM (10 years, 2 months ago) |
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First time wbs jars and got this on 1 out of 18 jars today. very small spot, pretty sure its green mold. Is it possible myc will take over or is it overwith?
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JoeMama1992
Asleep at the Wheel


Registered: 12/27/11
Posts: 901
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Re: Is it done for? [Re: Doost]
#19064742 - 10/31/13 05:22 PM (10 years, 2 months ago) |
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Bummer, but useless. PC that jar, toss out the grain, and keep a close eye on the rest of your jars.
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Doost
Stranger
Registered: 10/24/13
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Last seen: 9 years, 4 months
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I ended up losing 8 of the jars, 10 jars are looking 100% with no signs of contamination. Looking back over what I did everything seemed legit but possibly not waiting long enough to inoculate jars, is it possible that rushing inoculation could cause the contamination?
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JoeMama1992
Asleep at the Wheel


Registered: 12/27/11
Posts: 901
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Re: Is it done for? [Re: Doost]
#19096961 - 11/06/13 06:43 PM (10 years, 2 months ago) |
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Were they all from the same syringe? Really there shouldn't be any problem in rushing, as long as they were cool enough for the mycelium to survive. (Which they obviously were cool enough.) Did you flame sterilize the needle in between each jar?
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 10 hours, 3 minutes
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It usually gets in during the inoculation either from contaminated spores, from inoculating in open air especially during open lid inoculations, or from the needle becoming contaminated between the time you flame sterilize it and the inoculation if you used one.
It can also happen if the filter is damaged or wet but that's probably not the case considering where it's growing.
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Doost
Stranger
Registered: 10/24/13
Posts: 6
Last seen: 9 years, 4 months
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Quote:
JoeMama1992 said: Were they all from the same syringe? Really there shouldn't be any problem in rushing, as long as they were cool enough for the mycelium to survive. (Which they obviously were cool enough.) Did you flame sterilize the needle in between each jar?
I did flame sterilize inbetween every couple jars. Also used a alcohol swab between each one to cover the needle between each jar. Used 2 syringes, contams came pretty evenly between the two different one.
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JoeMama1992
Asleep at the Wheel


Registered: 12/27/11
Posts: 901
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Re: Is it done for? [Re: Doost]
#19107016 - 11/08/13 06:13 PM (10 years, 2 months ago) |
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And all incoulations were done in a glovebox/still-air box? If so, I'm just going to have to lean towards contaminated syringe. It looks like it's right at the area where the solution would've hit first anyway. Was the syringe from a trusted spore supplier, or just some random? You may want to bring it up to them in a way that doesn't sound like you're using their spores to grow.
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Kizzle
Misanthrope


Registered: 08/30/11
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Last seen: 10 hours, 3 minutes
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There's no way to know for sure how it got there. Contaminated spores are something you can run into even from trusted vendors unless you start using agar and when in a syringe contaminated spores usually means losing all or most of your jars. It's good reason to use spore prints instead of syringes when you can even if you're not going to clean them up on agar first IMO.
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Doost
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Registered: 10/24/13
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Last seen: 9 years, 4 months
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Quote:
JoeMama1992 said: And all incoulations were done in a glovebox/still-air box? If so, I'm just going to have to lean towards contaminated syringe. It looks like it's right at the area where the solution would've hit first anyway. Was the syringe from a trusted spore supplier, or just some random? You may want to bring it up to them in a way that doesn't sound like you're using their spores to grow.

I used the oven door method, always used it with my brf cakes. I've been getting my syringes from the same place for awhile now (sporedepot) and this was my first attempt at wbs. This was my second time seeing a contamination, last one was only one jar from a brf set that had black mold.
The remaining jars have been mixed into my tubs and see to be going off without any problems and have inoculated my next set of jars, no problems yet.
Thanks for the information and if I see a problem again will look into it again
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Kizzle
Misanthrope


Registered: 08/30/11
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Last seen: 10 hours, 3 minutes
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Re: Is it done for? [Re: Doost]
#19110416 - 11/09/13 01:32 PM (10 years, 2 months ago) |
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The oven door method is bunk. You'd be better off doing the inoculation in a draft free room. Even better would be in a still air box in a draft free room.
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