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OfflineMisterDeadeye


Registered: 09/02/11
Posts: 171
Last seen: 8 years, 8 months
First Agar Work -- Failure
    #19064501 - 10/31/13 04:43 PM (10 years, 2 months ago)

Well, I tried a bit of agar work the other day. No-pour tek, so I used the half pint Elite jars. Two were inoculated with a P. cubensis MS syringe and two others were inoculated with a P. galindoi print.

It was my first time using the glovebox I had made earlier in the day and I got so frustrated by the awkward shape and hole locations of the tub that I just took the lid off and did everything pretty much open air. That's why I only started out with 4 jars -- I assumed I was going to have troubles.

The worst part of it all? There was a clump of spores in the syringe and I accidentally pushed it out onto the surface of the tub and sucked it back up to inoculate the two jars. I know... I know...

The two cubensis jars grew trich, with one of them also growing some kind of bacteria I'll assume. Didn't see any healthy mycelium in either jar.

The two galindoi jars were at least white(slightly off, and I'm pretty sure it's bacteria). I haven't seen any galindoi mycelium IRL, but I'll assume it's not milky.



Regardless, I've built a new glovebox and plan on trying some more of the jars that I made. I figured all of the questions posted in this forum should be balanced out by someone talking about failure due to improper technique. Next time will be better.


Edited by MisterDeadeye (10/31/13 04:50 PM)


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OfflineMHbound
Ballin Out At All Cost
Male

Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 9 months
Re: First Agar Work -- Failure [Re: MisterDeadeye]
    #19064645 - 10/31/13 05:03 PM (10 years, 2 months ago)

Agar or liquid culture?


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OfflineMisterDeadeye


Registered: 09/02/11
Posts: 171
Last seen: 8 years, 8 months
Re: First Agar Work -- Failure [Re: MHbound]
    #19064717 - 10/31/13 05:17 PM (10 years, 2 months ago)

Agar. The jars I'm using are an alternative to sterilizing a flask and pouring Petris.


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OfflineMHbound
Ballin Out At All Cost
Male

Registered: 09/24/07
Posts: 6,512
Loc: Under The Rainbow
Last seen: 6 years, 9 months
Re: First Agar Work -- Failure [Re: MisterDeadeye]
    #19064753 - 10/31/13 05:24 PM (10 years, 2 months ago)

Oh ok I got you now. I was going to offer another alternative but don't have one for agar


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