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OfflineFractal420
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Dark clumps in syringes, toss?
    #19058420 - 10/30/13 03:49 PM (10 years, 3 months ago)

I got a pack of syringes that have been stored at room temp for a while. A few months later I look, and they've formed black spore clumps, and it seems even with heavy shaking I can't break them up. Are these basically gone bad and should be tossed? I read varying accounts on putting them in the fridge, heard it might kill the spores, but I'm just wondering, is there a way to use these safely or are they gone? Not a huge loss either way, would just like to know for future knowledge


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OfflineStromriderM
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Re: Dark clumps in syringes, toss? [Re: Fractal420]
    #19058429 - 10/30/13 03:51 PM (10 years, 3 months ago)

They're probably OK. Sometimes spores will germinate in a syringe. Shake it like a redheaded step child and use it


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OfflineKizzle
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Re: Dark clumps in syringes, toss? [Re: Stromrider]
    #19058479 - 10/30/13 04:00 PM (10 years, 3 months ago)

Quote:

I read varying accounts on putting them in the fridge, heard it might kill the spores



It won't hurt them.

Spores can form clumps. I wouldn't take it as meaning there's anything wrong with the spores.


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OfflineJMcDoogle
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Re: Dark clumps in syringes, toss? [Re: Kizzle]
    #19058499 - 10/30/13 04:05 PM (10 years, 3 months ago)

Yeah, the spores I got from a sponsor each had a tiny pea sized ( or smaller ) clump within them, jetblack...
give em a good shake and watch the beauty distrubute before you penetrate.. he he I said penetrate into your growth medium..

Almost poetic.
:nicesmile:


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InvisiblePirateSwazey
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Re: Dark clumps in syringes, toss? [Re: JMcDoogle]
    #19058659 - 10/30/13 04:42 PM (10 years, 3 months ago)

:seriousthumbsup:
they're safe to use.


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OfflineRubestoad
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Re: Dark clumps in syringes, toss? [Re: PirateSwazey]
    #19058706 - 10/30/13 04:50 PM (10 years, 3 months ago)

spores get lonely in the cold dark places we keep them so they group together for comfort.


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Invisibleanne halonium
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Re: Dark clumps in syringes, toss? [Re: Rubestoad]
    #19059182 - 10/30/13 06:41 PM (10 years, 3 months ago)

Quote:

Rubestoad said:
spores get lonely in the cold dark places we keep them so they group together for comfort.






note how they will form large herds.
they definitely like to cluster..


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:aliendance:


Edited by anne halonium (10/30/13 06:41 PM)


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OfflineFractal420
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Re: Dark clumps in syringes, toss? [Re: anne halonium]
    #19062526 - 10/31/13 10:03 AM (10 years, 3 months ago)

Thanks guys!

Would you say ~70ish room temp for syringes or fridge? I noticed the single one (from the same time) I put in the fridge is totally dispersed, the way it looks when you first get it and shake. Could the cold kill spores? Also, wouldn't the ones that have clumped be less even when they're squeezed out? I used a non-shaken syringe once without thinking, and it was a much slower development (although I was worried at the time, luckily they all turned out 100%, just not with the speed it normally would have with 4 sides, etc)


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OfflinecronicrFacebook
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Re: Dark clumps in syringes, toss? [Re: Fractal420]
    #19062538 - 10/31/13 10:06 AM (10 years, 3 months ago)

The fridge is fine


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OfflineKizzle
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Re: Dark clumps in syringes, toss? [Re: cronicr]
    #19063047 - 10/31/13 12:09 PM (10 years, 3 months ago)

I haven't seen any evidence to suggest the cold or even freezing hurts spores.


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Offlinerickjamez20
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Re: Dark clumps in syringes, toss? [Re: Kizzle]
    #19063112 - 10/31/13 12:25 PM (10 years, 3 months ago)

I'm pretty sure spores are hardy enough to survive the vacuum of space. They're tough little bastards.


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Offlineblueconfusion
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Re: Dark clumps in syringes, toss? [Re: rickjamez20]
    #19063136 - 10/31/13 12:29 PM (10 years, 3 months ago)

:whathesaid:


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Invisibleslapphappypill
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Re: Dark clumps in syringes, toss? [Re: blueconfusion]
    #19063407 - 10/31/13 01:19 PM (10 years, 3 months ago)

yeah, I've kept spores in the fridge for years... also prints in room temp... just fine either way


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Invisibleanne halonium
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Re: Dark clumps in syringes, toss? [Re: slapphappypill]
    #19063439 - 10/31/13 01:26 PM (10 years, 3 months ago)

3 things with spores........

i avoid freezing them fer sure.

a clean fridge is a very dry place.
so ya want your spores sealed well.

and,
a bio clean lab fridge, fine.
however,
the common household food fridge, is rife with dormant filth.

i have a dedicated lab fridge, but i use it for agar mostly.
i keep spores, sealed as in the pic above , at room temp ,
and, have for years...........


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:aliendance:


Edited by anne halonium (10/31/13 01:26 PM)


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OfflineJMcDoogle
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Re: Dark clumps in syringes, toss? [Re: anne halonium]
    #19063554 - 10/31/13 01:43 PM (10 years, 3 months ago)

Quote:

anne halonium said:
....
the common household food fridge, is rife with dormant filth.
...........




:whathesaid:


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OfflineFractal420
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Re: Dark clumps in syringes, toss? [Re: JMcDoogle]
    #19068606 - 11/01/13 06:02 AM (10 years, 3 months ago)

I, too, use a special fridge that only has bio stuff, no food "contaminating" the fridge :wink:


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OfflineRogerRabbitM
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Re: Dark clumps in syringes, toss? [Re: Fractal420]
    #19068683 - 11/01/13 06:56 AM (10 years, 3 months ago)

You can try setting the syringe in an ultrasonic jewelry cleaner.  That will sometimes break them up.  Otherwise, use as is and they just won't be spread out as well.
RR


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Invisiblejoiningheads
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Re: Dark clumps in syringes, toss? [Re: Fractal420]
    #19069915 - 11/01/13 01:25 PM (10 years, 3 months ago)

They haven't gone bad. In my experience, the clumping occurs over time with an undesirable surface tension of the liquid. By reducing this (Jetdry is most commonly recommended) it allows a more even spore distribution.  It pisses me off when vendors still haven't nailed this down. Back when I used to buy syringes, it was like a 50/50 shot whether they would have this characteristic, and although it never hurt it tends to clog and become a nuisance.


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