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Offlinecatmando
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Registered: 09/18/13
Posts: 21
Last seen: 5 years, 10 months
no growth after shake
    #19063109 - 10/31/13 12:24 PM (10 years, 2 months ago)

Alright, I have had two spawn bags colonizing for the past six weeks, they are three pound rye bags.  I mixed them up on the 25th, today almost a week later there is no growth.

the bags were about 20-30% colonized when i shook them

any idea whey they are taking so long to recover

also they had been colonizing slowly to begin with, almost six weeks and only 20%


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Offlineblueconfusion
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Re: no growth after shake [Re: catmando]
    #19063115 - 10/31/13 12:25 PM (10 years, 2 months ago)

Possibly bacteria


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OfflinePsychedelicScience
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Re: no growth after shake [Re: blueconfusion]
    #19063157 - 10/31/13 12:33 PM (10 years, 2 months ago)

Quote:

blueconfusion said:
Possibly bacteria




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InvisiblePsiLisaBin
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Registered: 07/11/13
Posts: 128
Re: no growth after shake [Re: PsychedelicScience]
    #19063188 - 10/31/13 12:40 PM (10 years, 2 months ago)

this exact same thing happened to me once before
bacteria was the only conclusion i could come to, absolutely no growth of any kind in any jar after doing a g2g
it's only happened to me once though, it was the wierdest thing


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Invisible36fuckin5
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Registered: 08/11/03
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Re: no growth after shake [Re: PsiLisaBin]
    #19063207 - 10/31/13 12:44 PM (10 years, 2 months ago)

Sounds like bacteria to me too, but no matter what it is, it's not worth using.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Offlineslartibartfast
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Re: no growth after shake [Re: PsiLisaBin]
    #19063209 - 10/31/13 12:45 PM (10 years, 2 months ago)

Take a good sniff around the filter patch and it should be easy enough to tell weather bacteria are causing your problem. Any sweet rotten apple type smells pretty much mean that bacteria have managed to overgrow your bags. How long where they PC'd for and what temp you incubating at?


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Pressure cooking shit to kill shit so i can knock it up with my shit to grow shit.
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Offlinecatmando
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Registered: 09/18/13
Posts: 21
Last seen: 5 years, 10 months
Re: no growth after shake [Re: slartibartfast]
    #19063256 - 10/31/13 12:50 PM (10 years, 2 months ago)

I bought them pre-sterilized before i got my pressure cooker

and they have been at room temp around 72 degrees


EDIT  the product description states they were pcd at 15psi for 120 min


Edited by catmando (10/31/13 12:52 PM)


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Offlinecatmando
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Re: no growth after shake [Re: catmando]
    #19063292 - 10/31/13 12:56 PM (10 years, 2 months ago)

just gave em a good sniff and i believe you all were right, smells pretty sour sweet rotteny

fuck...

The place i ordered from guaranties their bags are 100% sterile so does that mean they should replaced these bags since they went bad?


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Invisibleslapphappypill
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Re: no growth after shake [Re: catmando]
    #19063441 - 10/31/13 01:26 PM (10 years, 2 months ago)

The bacteria could have cam when you did the g2g or knocked up your jars to begin with... practice your sterile technique and try again :-)

Also, since you have a PC, don't buy anymore pre-made jars or bags... WAY more expensive than needed!


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~


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Offlinecatmando
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Last seen: 5 years, 10 months
Re: no growth after shake [Re: slapphappypill]
    #19064340 - 10/31/13 04:19 PM (10 years, 2 months ago)

yeah, no more pre sterilized bs.  bought them before i decided to buy a pc

is it possible that the bags were prepared too wet?  isnt that a major cause of bact?

I practiced pretty strict sterile technique, but there is always room for improvement


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OfflinecronicrFacebook
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Re: no growth after shake [Re: catmando]
    #19064357 - 10/31/13 04:21 PM (10 years, 2 months ago)

It is possible they were too wet but that's the benifit of doing it yourself, you would have known


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Offlinecatmando
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Registered: 09/18/13
Posts: 21
Last seen: 5 years, 10 months
Re: no growth after shake [Re: cronicr]
    #19064402 - 10/31/13 04:28 PM (10 years, 2 months ago)

yeah i know that doing it myself is better, ive got 14 jars of rye going right now that ive prepped myself.


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