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TheLoopIs
Registered: 05/29/13
Posts: 330
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Why am I getting no growth?
#19062287 - 10/31/13 09:00 AM (10 years, 2 months ago) |
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I knocked up 3 jars 3 days ago and I'm not seeing any growth. Used pan cyans, and shittake and rye seeds in pre sterilized, "fancy" mason jars. I think I remember shaking up every one of them when I inoculated cause I thought it would help. Did I screw it up completely by doing this, or will the spores eventually find each other no matter what and reproduce?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Re: Why am I getting no growth? [Re: TheLoopIs]
#19062296 - 10/31/13 09:02 AM (10 years, 2 months ago) |
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Spores can take 2+ weeks to show growth.....
How much spore solution did you use per jar?
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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TheLoopIs
Registered: 05/29/13
Posts: 330
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Re: Why am I getting no growth? [Re: PussyFart]
#19062312 - 10/31/13 09:05 AM (10 years, 2 months ago) |
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around 1.5 cc. Thanks nothacker420 I'll take it as a golden opportunity to practice patience:D
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Re: Why am I getting no growth? [Re: TheLoopIs]
#19062316 - 10/31/13 09:06 AM (10 years, 2 months ago) |
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You should be fine.
Now if you only used a few drops I would worry....but 1.5cc shaken should still work.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Why am I getting no growth? [Re: PussyFart]
#19062506 - 10/31/13 09:58 AM (10 years, 2 months ago) |
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For future reference don't shake after inoculation especially with MS syringe. But like NaH said you should be fine.
Pan's are slightly more picky about how they like to be cultivated. How are you planing on fruiting them?
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TheLoopIs
Registered: 05/29/13
Posts: 330
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Re: Why am I getting no growth? [Re: bodhisatta]
#19068727 - 11/01/13 07:23 AM (10 years, 2 months ago) |
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bodhista I was thinking of just seeing if they would fruit on the cake?
If not I have cow manure on hand to mix it in with the broken up cake? I don't have a Pressure Cooker though, and last time I used manure as substrate all of my 6 jars got a contam:( Do you guys have experience with nooking manure in the microwave for this purpose?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Why am I getting no growth? [Re: TheLoopIs]
#19068868 - 11/01/13 08:54 AM (10 years, 2 months ago) |
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I don't know shit about pan cyans but I'm pretty sure you need poo to make them fruit. You'll never steam sterilize unless you want to wait way more than 90 minutes. Perhaps you could properly pasteurize it and spawn the cakes to it.
Don't quote me on this it might be completely wrong. You should check the search engine.
The microwave is good for nothing in this hobby especially sterilization. The microwave is good for melting agar that's about it.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Why am I getting no growth? [Re: bodhisatta]
#19069087 - 11/01/13 10:12 AM (10 years, 2 months ago) |
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If you have verm for your cow poo just 50/50 that and pasturize, pans prefer manure but will fruit off other subs
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TheLoopIs
Registered: 05/29/13
Posts: 330
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Re: Why am I getting no growth? [Re: cronicr]
#19071179 - 11/01/13 05:39 PM (10 years, 2 months ago) |
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bodhisatta what do u mean by properly pasteurizing? Thanks for the tip cronic u the best!
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 17 hours, 55 minutes
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Re: Why am I getting no growth? [Re: TheLoopIs]
#19071251 - 11/01/13 05:53 PM (10 years, 2 months ago) |
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Why am I getting no growth? [Re: Stromrider]
#19071618 - 11/01/13 07:11 PM (10 years, 2 months ago) |
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