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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Why am I getting no growth?
    #19062287 - 10/31/13 09:00 AM (10 years, 2 months ago)

I knocked up 3 jars 3 days ago and I'm not seeing any growth.
Used pan cyans, and shittake and rye seeds in pre sterilized, "fancy" mason jars.
I think I remember shaking up every one of them when I inoculated cause I thought it would help. Did I screw it up completely by doing this, or will the spores eventually find each other no matter what and reproduce?


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
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Re: Why am I getting no growth? [Re: TheLoopIs]
    #19062296 - 10/31/13 09:02 AM (10 years, 2 months ago)

Spores can take 2+ weeks to show growth.....

How much spore solution did you use per jar?


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Why am I getting no growth? [Re: PussyFart]
    #19062312 - 10/31/13 09:05 AM (10 years, 2 months ago)

around 1.5 cc.  Thanks nothacker420 I'll take it as a golden opportunity to practice patience:D


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OfflinePussyFart
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Re: Why am I getting no growth? [Re: TheLoopIs]
    #19062316 - 10/31/13 09:06 AM (10 years, 2 months ago)

You should be fine.

Now if you only used a few drops I would worry....but 1.5cc shaken should still work.


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Why am I getting no growth? [Re: PussyFart]
    #19062506 - 10/31/13 09:58 AM (10 years, 2 months ago)

For future reference don't shake after inoculation especially with MS syringe. But like NaH said you should be fine.

Pan's are slightly more picky about how they like to be cultivated. How are you planing on fruiting them?


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Why am I getting no growth? [Re: bodhisatta]
    #19068727 - 11/01/13 07:23 AM (10 years, 2 months ago)

bodhista I was thinking of just seeing if they would fruit on the cake?

If not I have cow manure on hand to mix it in with the broken up cake? I don't have a Pressure Cooker though, and last time I used manure as substrate all of my 6 jars got a contam:( Do you guys have experience with nooking manure in the microwave for this purpose?


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InvisiblebodhisattaMDiscordReddit
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Re: Why am I getting no growth? [Re: TheLoopIs]
    #19068868 - 11/01/13 08:54 AM (10 years, 2 months ago)

I don't know shit about pan cyans but I'm pretty sure you need poo to make them fruit. You'll never steam sterilize unless you want to wait way more than 90 minutes. Perhaps you could properly pasteurize it and spawn the cakes to it.

Don't quote me on this it might be completely wrong. You should check the search engine.


The microwave is good for nothing in this hobby especially sterilization. The microwave is good for melting agar that's about it.


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OfflinecronicrFacebook
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Re: Why am I getting no growth? [Re: bodhisatta]
    #19069087 - 11/01/13 10:12 AM (10 years, 2 months ago)

If you have verm for your cow poo just 50/50 that and pasturize, pans prefer manure but will fruit off other subs


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I'm tired do me a favor


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Why am I getting no growth? [Re: cronicr]
    #19071179 - 11/01/13 05:39 PM (10 years, 2 months ago)

bodhisatta what do u mean by properly pasteurizing? 
Thanks for the tip cronic u the best!


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OfflineStromriderM
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Re: Why am I getting no growth? [Re: TheLoopIs]
    #19071251 - 11/01/13 05:53 PM (10 years, 2 months ago)



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InvisibleSpitballJedi
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Re: Why am I getting no growth? [Re: Stromrider]
    #19071618 - 11/01/13 07:11 PM (10 years, 2 months ago)

:whathesaid::whathesaid:


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