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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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1st attempt agar - hope all goes well
#19062271 - 10/31/13 08:53 AM (10 years, 2 months ago) |
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sorry tried my best on the pictures shitty camera, and shitty user.

these was started on the 10/26
are they ok to shake for faster speed?
so far everything looks clean no ugly colors. my hope to make 40 g2g jars.
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Edited by Yuri.Pono (10/31/13 08:54 AM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: 1st attempt agar - hope all goes well [Re: Yuri.Pono]
#19062510 - 10/31/13 09:59 AM (10 years, 2 months ago) |
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Looks good. It also looks like you shook some of those jars up already. Wait till at least 30% even when starting from an agar wedge before shaking.
I wouldn't shake any of them yet
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
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Re: 1st attempt agar - hope all goes well [Re: Yuri.Pono]
#19062576 - 10/31/13 10:17 AM (10 years, 2 months ago) |
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Quote:
Yuri.Pono said: sorry tried my best on the pictures shitty camera, and shitty user.

these was started on the 10/26
are they ok to shake for faster speed?
so far everything looks clean no ugly colors. my hope to make 40 g2g jars.
Pictures look clean enough, shitty user.
Which Brings me to an observation -
Unlike me, you've got this far with seven posts - which to me says you've done a lot of reading, and you follow advice and directions of those who've gone down the road before you.
For that - Five Shrooms.
Another observation is that you've spent a little money on either ez-felt or SFD ( synthetic filter discs ) for your jars, another step even I didnt take in the beginning. Some say its not neccesary, some say its a time/contam saver. I like it.
And you've done agar instead of asking fourty times why LC isnt a good idea.
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The ego is nothing other than the focus of conscious attention.
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: 1st attempt agar - hope all goes well [Re: JMcDoogle]
#19062845 - 10/31/13 11:17 AM (10 years, 2 months ago) |
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ya i haven't done a shake yet just happened to be where the agar landed and thanks for the positive feed back
just seems to be moving a little slow, but doesn't everything in this game.
ive been reading a ton b4 starting. yes you are correct sfd's followed a few teks here. for some reason i keep thinking that i read something from RR where he said wait for 100 grains to colonize b4 shaking roughly the size of a quarter. just cant seem to find the thread in a search. anyways think ill wait as that would create more inoculation points at 30%.
just thought i also read you could have 100% colonized jars in a week with agar.
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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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Re: 1st attempt agar - hope all goes well [Re: Yuri.Pono]
#19062888 - 10/31/13 11:25 AM (10 years, 2 months ago) |
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Just guessing but could you possibly be working with some sort of mexicana truffle producer?
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: 1st attempt agar - hope all goes well [Re: spaceman101]
#19062970 - 10/31/13 11:49 AM (10 years, 2 months ago) |
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Escondido. why do you think not cubes just curious.
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: 1st attempt agar - hope all goes well [Re: Yuri.Pono]
#19062982 - 10/31/13 11:52 AM (10 years, 2 months ago) |
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Quote:
Those look fine, but you would have been better off not to shake as directed above.
The reason is that when you cut off a wedge, you cut through the growing, leading edge of the mycelium. It's best to let this recover without further damage, so that the mycelium crawls off the wedge and into the grains below. It makes little difference if the wedge is upside down or right side up because it's not going to recover and grow from the middle, but from the leading edges.
Once the mycelium has crawled into a few of the grains below, shake to distribute. Shaking early with a mycelium wedge slows things down because you only get recovery later from the wedge itself, not from all the mycelium which falls off when you shake, but doesn't recover and grow. You must then wait for what's left on the agar wedge to recover and grow again before the mycelium can leap off, setting things back a few days.
The other advantage to not shaking right away is that you can see the agar wedge on top so that if green mold or another contaminant develops, you see it before you shake so you can discard that jar. RR
please forgive i was wrong
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: 1st attempt agar - hope all goes well [Re: Yuri.Pono]
#19151572 - 11/17/13 08:07 PM (10 years, 2 months ago) |
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so i built a TiT incubator and noticed that the jar that was stuck in the corner had metabolites im guessing. temps did hit 84 degrees when i notice this so im guessing it is from high temps.
fixed up picture

original picture

other side of jar

the rest of jars are great working on soak for g2g for the rest
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
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Re: 1st attempt agar - hope all goes well [Re: Yuri.Pono]
#19151584 - 11/17/13 08:10 PM (10 years, 2 months ago) |
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Quote:
RogerRabbit said: It looks like metabolites, but there's no such thing as myc piss. Metabolites serve two functions. One is to help break down the substrate with solvents, and the other is an antibiotic function to help fight off competitors. Mycelium doesn't urinate, and the metabolites are not a waste product, despite what you read elsewhere. Penicillin is produced from the metabolites of another species of fungi. You certainly wouldn't ask your doctor for a shot of myc piss if you had the clap. RR
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The ego is nothing other than the focus of conscious attention.
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Yuri.Pono
MAD SCIENTIST


Registered: 10/28/13
Posts: 279
Loc: SO HIGH O
Last seen: 9 years, 9 months
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Re: 1st attempt agar - hope all goes well [Re: JMcDoogle]
#19160781 - 11/19/13 05:20 PM (10 years, 2 months ago) |
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Quote:
A large amount of metabolites generally means a bit of bacteria in the jar. The metabolites are antibiotic secretions, so when they're in large amounts, it usually means something is up. High colonization temps will also lead to metabolite production, so remember to colonize jars at room temperature.
A jar with a good filter will contain any contaminants that are within, so don't toss it out. Don't use any jar with a lot of metabolites for grain to grain transfers, but if they colonize fine, they're good to use for spawning to bulk. RR
found this guess ill be making a shoebox bulk for now and g2g the rest
Quote:
so remember to colonize jars at room temperature.
Quote:
72F to 80F is fine. Some growers incorrectly 'incubate' at 86F, which causes all sorts of problems. RR
Quote:
You won't get metabolites pooling in the bottom of a jar before full colonization unless it's contaminated. Excess heat will cause metabolites to build up too, but only after full colonization.
quote noted
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Edited by Yuri.Pono (11/19/13 05:36 PM)
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