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Turbogoose
"Uncolonized"
Registered: 10/28/13
Posts: 4
Last seen: 5 years, 9 months
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No mycelium growth
#19060728 - 10/30/13 10:17 PM (10 years, 2 months ago) |
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Hello Shroomery,
This is my first post, so if there is a welcome thread or something I missed posting in, please turn me in the right direction and send me on my way. That all being said, here is my setup and issues:
I checked out a few versions of BRF tek, but basically went with the RR video version and followed all the steps. I think I was pretty good about sterilization, I made a (crappy) glove box, rubbed every surface down with 91% isopropyl alcohol, did the 90 minute boil, (no pressure cooker), etc.
After 2 weeks and a day, 2 of my jars are showing clear mycelium growth, but none of the others are out a total of 11, (6 with B+, 5 with Golden Teacher, both from a reliable Shroomery vendor, so the spores are not the problem). I think my problem is that I did not let the jars cool enough before inoculation after sterilization.
My question is this: Should I throw out the 9 non-active jars or am I being impatient? The 2 jars with growth showed within 6 days of inoculation, and all of the jars were inoculated toward the glass, not in the center. The 2 growth jars are noticeably warmer to the touch than the other 9. The 9 do not have any discoloration, noticeable mold/contamination, or strange smells.
I do not know how likely it is that bacteria/mold could spread from one of the non-growth jars to the growth jars, but I want to at least have this small measure of success, so I am concerned.
Apologies if this has been asked before, I didn't find my exact concerns addressed in any of the other multitude of "NO GROWTH!!!" threads.
Thanks in advance.
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flipsidetrue




Registered: 05/26/13
Posts: 364
Loc: Earth
Last seen: 7 years, 10 months
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how long did u let the jars cool down after you inoculated them?
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Rubestoad
Stranger


Registered: 09/13/13
Posts: 231
Loc: your mamas porch. sunrise
Last seen: 8 years, 3 months
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If they don't look or smell bad don't throw them out yet give it time bro
-------------------- Go placidly amid the noise and haste, and remember what peace there may be in silence.
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mirvmenace
Stranger

Registered: 10/24/13
Posts: 23
Last seen: 10 years, 1 month
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Re: No mycelium growth [Re: Rubestoad]
#19061596 - 10/31/13 02:45 AM (10 years, 2 months ago) |
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i had the same problem with my first batch. I tried to be more sterile and i used a pressure cooker. I got mine on sale for $20 at bed bath. After i PCed it and inoculated, mycelium began to grow a week later
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,343
Loc: The Inexpressible...
Last seen: 7 hours, 4 minutes
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Yeah, if only 2 out of 11 are showing mycelium, u must have cooked the spores.
Ive done it before, and it SUCKS. Letting the jars cool for at least 10 hours (overnight is best) is so important. Glass acts as an insulator, so it takes a long time for cooling.
But it could also be a weak syringe. I have had a weak syringe before. Basically, its a syringe with very little spores, so it doesnt perform well in jars. So it could be that. Make sure you syringe was from a good vendor. And if it was cheap, then it likely is cheap, ya know what I mean?
New syringe and try again.
And dont forget to cool them long time! ~ LC
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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70% alcohol is better and id start more jars, least you got a couple
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blueconfusion
Strangest



Registered: 12/14/12
Posts: 1,727
Last seen: 4 days, 21 hours
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Re: No mycelium growth [Re: cronicr]
#19062636 - 10/31/13 10:31 AM (10 years, 2 months ago) |
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Along with cooling make sure to give your syringe a good shake before inoculation
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Turbogoose
"Uncolonized"

Registered: 10/28/13
Posts: 4
Last seen: 5 years, 9 months
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Thanks to all the replies!
Definitely sounds like I cooked them. I don't think I even waited an hour and a half before inoculation, so the two growing are probably the last two I did. I shook the spores up and the syringes were nice and cloudy; definitely my bad, not the vendors, (in fact, I was quite happy with my purchase! The fact that ANY grew without letting the jars cool says something about the quality).
Next question: When I give this another go-around, should I let the jars cool at: a.) Room Temp b.) In the fridge
Also, do I need to leave the foil lid on when I let them cool?
Thanks again, we shall see how the two good ones turn out! I think, just to be safe, I'm going to boil the other jars tonight and dump them out; I don't want to risk contamination in my only 2 good jars!
Thanks again!
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Turbogoose
"Uncolonized"

Registered: 10/28/13
Posts: 4
Last seen: 5 years, 9 months
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Re: No mycelium growth [Re: cronicr]
#19070249 - 11/01/13 02:40 PM (10 years, 2 months ago) |
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Quote:
cronicr said: 70% alcohol is better and id start more jars, least you got a couple
Could you please explain why this is?
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Rubestoad
Stranger


Registered: 09/13/13
Posts: 231
Loc: your mamas porch. sunrise
Last seen: 8 years, 3 months
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Your fridge is full of nastys you shouldn't put them in there...
-------------------- Go placidly amid the noise and haste, and remember what peace there may be in silence.
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barada42
Stranger
Registered: 09/16/13
Posts: 19
Last seen: 7 years, 2 months
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If there's no contam in the jars, and the only problem was not letting them cool long enough, why not just inoculate them now?
Sterile is sterile is it not? And if they were too hot when you inoc'd then there's even less chance that contams have been introduced. Maybe another quick PC and cooldown beforehand if you're worried...?
-------------------- -- Everything above this line is fiction
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Quote:
Turbogoose said:
Quote:
cronicr said: 70% alcohol is better and id start more jars, least you got a couple
Could you please explain why this is?
Because the higher water content will fool cells to allow it in and do its job better which is the same reason we humans use it and not the 91. Rub some of both on your skin and you will see what I mean
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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OneiricOutsider
Drucid Leamer


Registered: 10/05/13
Posts: 136
Loc: In the autumn shade
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Re: No mycelium growth [Re: barada42]
#19070974 - 11/01/13 05:08 PM (10 years, 2 months ago) |
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Quote:
barada42 said: If there's no contam in the jars, and the only problem was not letting them cool long enough, why not just inoculate them now?
Sterile is sterile is it not? And if they were too hot when you inoc'd then there's even less chance that contams have been introduced. Maybe another quick PC and cooldown beforehand if you're worried...?
The problem wouldn't be the sterility, but rather the fact that you'd be throwing off the water content, which could lead to some contamination problems down the road. He's better off just starting over. Also, just cool them at room temp, and I always leave the foil on the jars while they cool, never had any problems.
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Turbogoose
"Uncolonized"

Registered: 10/28/13
Posts: 4
Last seen: 5 years, 9 months
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Thanks again,
I re-sterilized and tossed the jars. I didn't have any syringes to use anyway. Can I dilute my 91% alcohol with distilled water? Otherwise, I think I'm set to prepare the jars again and start a late batch!
Thanks everyone, hopefully I will be around on this forum for awhile. I appreciate the helpful nature you all seem to have.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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someone had a chart about how much wayer to add....hmmm search around though
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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