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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Vermiculite, Wild Bird Seed

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Offlinelysergic
Mycophile!
Registered: 06/09/03
Posts: 691
Last seen: 13 years, 9 days
PF cake to grain transfer
    #1905845 - 09/11/03 04:06 PM (13 years, 2 months ago)

Is it possible to cut up a PF cake into pea-sized chunks of mycelia and use these to inocluate larger sized jars filled with sterile WBS? How difficult is this?


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In response to an attack killing 15 American Servicemen
PsiloKitten said:
Just give em a little more time, the iraqis are making great progress. And this is unorganized. Wait till they get organized.


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Offlineprocule
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Re: PF cake to grain transfer [Re: lysergic]
    #1905891 - 09/11/03 04:14 PM (13 years, 2 months ago)

of course you can.

The difficulty results of your ability to do the transfer in a sterile environment.


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home grown shit
god damn it


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OfflineGanoderma
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Registered: 09/05/03
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Re: PF cake to grain transfer [Re: procule]
    #1906023 - 09/11/03 04:36 PM (13 years, 2 months ago)

Or the ability to transfer a contaminate free substrate to a sterilized substate without introducing any contamination.
IF your PF cake has any, ANY contamination all jars spawned via that tranfer will be doomed!
sugestions, 1. a glove box, 2. build an h.e.p.a. filter into your glove box w/with the air blowing out towards you. 3. filter disks to place on jars so you can shake the grain to speed colinization, 4. agar and dishes, makes it easier to spawn contaminate free grain masters


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OfflinePooPs
What's thisfor???

Registered: 03/05/03
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Re: PF cake to grain transfer [Re: Ganoderma]
    #1906131 - 09/11/03 04:55 PM (13 years, 2 months ago)

consider transfering your cake from the jar into a new/clean baggie or ziplock.. crush it up from the outside of the bag... then use a spoon dipped in straight peroxide to transfer it to the grain jars!!! dip your spoon between transfers and don't bother to wipe it down... the little bit of h2o2 will help keep contams at bay!


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Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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Offlineshroomstruck
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Registered: 08/06/03
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Re: PF cake to grain transfer [Re: PooPs]
    #1906474 - 09/11/03 06:23 PM (13 years, 2 months ago)

My friend transfered his first pf cake to popcorn 3 nights ago.

Similar to PooPs approach he dumped the cake into ziplock to break up the cake. He dumped 4 pint jars of pc'd popcorn into an oven bag and then added the crumbled pf cake.

He could have done this in one bag, but this was his first pf cake and wasn't sure how it would crumble .... he also wanted to sniff at it!

Things are growing and no sign of contams yet!
btw I added a tyvek filter after this photo


Edited by shroomstruck (09/11/03 06:25 PM)


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Anonymous

Re: PF cake to grain transfer [Re: lysergic]
    #1908657 - 09/12/03 05:45 AM (13 years, 2 months ago)

Quote:

lysergic said:
Is it possible to cut up a PF cake into pea-sized chunks of mycelia and use these to inocluate larger sized jars filled with sterile WBS? How difficult is this?




I rescently did g2g and had pretty good success 6 outta 7 jars made it.....I used tweezers which I swabbed down with alcohol for the transfer....and had the pieces of cake floating in h20/h202 before I transfered them


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InvisibleWheedhead!
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Registered: 11/14/02
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Re: PF cake to grain transfer [Re: shroomstruck]
    #1908747 - 09/12/03 08:06 AM (13 years, 2 months ago)

Sorry if im wrong shroomstruck. But the bag of popcorn on your picture looks like its comtaminated with green mold.  :sad:

Am i wrong fellow shroomers? 




 



 


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Anonymous

Re: PF cake to grain transfer [Re: Wheedhead!]
    #1908774 - 09/12/03 08:20 AM (13 years, 2 months ago)

oh I didn't even realize he thought they were ok....I thought he was posting his failure.....definitely contamed I'd say unless you got some funky lighting going on or something


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InvisibleStarter
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Re: PF cake to grain transfer [Re: ]
    #1908824 - 09/12/03 09:05 AM (13 years, 2 months ago)

In the thumbnail it looks like the dreaded green stuff, but in the full pic it looks likes the grey colour of damp vermiculite with white mycelium bits in it.


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Convert Metric and Imperial.


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OfflinePooPs
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Re: PF cake to grain transfer [Re: ]
    #1908826 - 09/12/03 09:08 AM (13 years, 2 months ago)

Yeah.. thought the same.. i hope they are chunks of vermiculite!...


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-----------
Sniff, Sniff... What's that smell???... ohhhhh.!!
------------------


Pot Free for another : nevermind.. never made it..


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Anonymous

Re: PF cake to grain transfer [Re: Starter]
    #1908871 - 09/12/03 10:04 AM (13 years, 2 months ago)

I've never seen vermiculite get that dark before but it could be the brand I use


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Offlinechessrook
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Registered: 02/03/03
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Re: PF cake to grain transfer [Re: lysergic]
    #1909041 - 09/12/03 11:33 AM (13 years, 2 months ago)

Yes, I know of a friend who used a simple glovebox to do what you are asking. He birthed a BRF cake in the glovebox and crumbled and transferred to 3 WBS (qt) jars. This was more of a test of his glovebox design and technique than an attempt to spawn 3 jars. He's new at it.
Anyway, 1 jar fell to the green, but 2 made it to full colonization. It took awhile for the jars to colonize though (probably bad water content in WBS). He tried the same thing with popcorn. The popcorn caught, bypassed, and cased before WBS colonized, 0 contam losses. So, I hope that helps answer in the affirmative.


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Offlineshroomstruck
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Registered: 08/06/03
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Re: PF cake to grain transfer [Re: chessrook]
    #1909064 - 09/12/03 11:46 AM (13 years, 2 months ago)

Thanks for the concern everyone ... they are the brown chunks of vermiculite & rice.
Does this jar look darker than usual? This is it before crumbling


The bag was inspected this morning and looks good.



My attemt to clone oysters ended in contamination, so I now know what the goddamn green mold looks like.  :frown: 


Edited by shroomstruck (09/12/03 11:51 AM)


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Invisiblear393
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Re: PF cake to grain transfer [Re: shroomstruck]
    #1909080 - 09/12/03 11:53 AM (13 years, 2 months ago)

hey just to let you know...make sure that your oven bag isnt too breathable or your popcorn will dry out.
-ar393


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OfflineBeppoMarx
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Re: PF cake to grain transfer [Re: ar393]
    #1909239 - 09/12/03 12:49 PM (13 years, 2 months ago)

lysergic, just be sterile as possible, adding too many steps will just add more chances of contamination. build a glovebox with a cardboard box, duct tape, and a $2 plexiglass sheet from a glass/window store or maybe chase pitkin or something.

shroomstruck, youre ok :smile: that cake looked great and those kernels should take off well of youve steeped them well :smile:


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Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
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Invisiblegeorge castanzaM
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Re: PF cake to grain transfer [Re: BeppoMarx]
    #1909469 - 09/12/03 02:11 PM (13 years, 2 months ago)

the only thing you can do is try it and see...
it looks like you have done most of your homework.
kramers advise is to do small amounts at first so you dont wind up tossing hundreds of pounds of substrate in the trash


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Quote:

pftek said:Impossible to clean everything right now. Maybe in a week.





ROTHBURY

:pm:


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Offlineshroomstruck
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Registered: 08/06/03
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Re: PF cake to grain transfer [Re: george castanza]
    #1910640 - 09/12/03 07:57 PM (13 years, 2 months ago)

That was the point I was trying to make about simplicity.
I felt there was less chance of contamination If I never exposed the cake or grain to anything but plastic bag.
But if you have a real glovebox you're golden! :smile:

Thanks guys I'm very excited and can't wait for the next step :smile:
While I wait for straw time this will be duplicated using karo kulture instead of pf cake.
The pc is very small, so I can't think in Kramer's terms. :smile:
I'll post a thread when I have more pics.


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Offlinelysergic
Mycophile!
Registered: 06/09/03
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Re: PF cake to grain transfer [Re: shroomstruck]
    #1910676 - 09/12/03 08:14 PM (13 years, 2 months ago)

Shroomstruck - CongradulationS! and thanks for the help, a rating of five from me to you :smile: 

In theory, this experiment, conducted with legal, edible mushrooms, would be done in a glove box that has reduced the number of ocntams from nearly 40% failure rate (in BRF/VERM style pint jars) to zero.  I think that it'll just be a test of aseptic conditions. thanks for the 411


--------------------
In response to an attack killing 15 American Servicemen
PsiloKitten said:
Just give em a little more time, the iraqis are making great progress. And this is unorganized. Wait till they get organized.


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Offlineshroomstruck
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Registered: 08/06/03
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Re: PF cake to grain transfer [Re: lysergic]
    #1910829 - 09/12/03 09:40 PM (13 years, 2 months ago)

WOW, Lysergic, thanks for the 5 shrooms .. that's so cool of you, bud! :smile:

My feeling about gloveboxes is unless it is a very tight one (or like Ganoderma's) it isn't much more effective than a new plastic garbage bag ... which is pretty sterile from the start.
I read a lot before deciding my tech and felt that moving that spoon around was an antenna for contams, unless you were very sure of yourself... and I'm just a newbie.
Good luck, whichever way you go .. show some pics!  :smile:

If the lo end tech goes full circle you can get the first print! :smile:





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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Vermiculite, Wild Bird Seed

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