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Offlinelinguafranca
Virgin cultivator
Male

Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: Torgo2212]
    #19059221 - 10/30/13 06:50 PM (10 years, 2 months ago)

Well that was extremely reassuring, thank you. I guess all I can do now is hope that Jeff Goldblum was right in Jurassic Park.


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Offlinelinguafranca
Virgin cultivator
Male

Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19170443 - 11/21/13 02:50 PM (10 years, 2 months ago)

I have been keeping an eye on the jars for the past couple weeks and I haven't seen any growth. I'm gonna check one more time when I get back, but it looks like I did screw it up :frown:


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Offlinelinguafranca
Virgin cultivator
Male

Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19170783 - 11/21/13 03:48 PM (10 years, 2 months ago)

okay a little bit of news to dampen the blow - I found that three of my jars are actually colonizing! One is really early state (I believe I saw it collecting in the middle of the jar), one has completely colonized the topside of the jar, and one has almost gotten the entire jar. I had originally 18 jars going so this wasn't a huge success, but, dammit, it's something :grin:


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Offlinelinguafranca
Virgin cultivator
Male


Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19170937 - 11/21/13 04:13 PM (10 years, 2 months ago)




here are the two jars that I know for sure have growth in them


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
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Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19170978 - 11/21/13 04:19 PM (10 years, 2 months ago)

Remove the foil....let the dry verm layer do it's job....


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Offlinelinguafranca
Virgin cultivator
Male


Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: PussyFart]
    #19171114 - 11/21/13 04:46 PM (10 years, 2 months ago)

Wait, what? I thought I had to keep the foil on them. I've kept the foil on all the jars the entire time. Do you think that has something to do with the slow growth?


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Last seen: 17 days, 13 hours
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Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19171128 - 11/21/13 04:48 PM (10 years, 2 months ago)

Foil gets removed after sterilization and stays off.

It should not hinder GE, but it could moisten the dry verm layer, which is not good.

This is why it is recommended to keep the foil off, so the dry verm layer can stay dry.


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Offlinelinguafranca
Virgin cultivator
Male


Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: PussyFart]
    #19171142 - 11/21/13 04:50 PM (10 years, 2 months ago)

wow. okay then, well my jars have all had the foil on since inoculation, should I just take them all off including the ones that are colonizing or what? I'm afraid my dry layer might be compromised now since it's been so long


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OfflinePussyFart
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Registered: 04/08/12
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Re: First timer and extremely nervous about inoculation [Re: PussyFart]
    #19171144 - 11/21/13 04:51 PM (10 years, 2 months ago)

Quote:

linguafranca said:
wow. okay then, well my jars have all had the foil on since inoculation, should I just take them all off including the ones that are colonizing or what? I'm afraid my dry layer might be compromised now since it's been so long



Quote:

Notahacker420 said:
Remove the foil




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Offlinelinguafranca
Virgin cultivator
Male


Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: PussyFart]
    #19171209 - 11/21/13 05:01 PM (10 years, 2 months ago)

I just removed all the foil off of them. The two jars that currently have a sizable grow have the top layer of the vermiculite being consumed into the cake because of an error during inoculation.there isn't anything protecting them now and i'm sorta scared


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Edited by linguafranca (11/21/13 05:05 PM)


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Invisibled0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
Re: First timer and extremely nervous about inoculation [Re: PussyFart]
    #19171578 - 11/21/13 06:06 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Foil gets removed after sterilization and stays off.

It should not hinder GE, but it could moisten the dry verm layer, which is not good.

This is why it is recommended to keep the foil off, so the dry verm layer can stay dry.




I've always heard people say leave the foil off for the GE not to prevent dry verm layer from getting wet. This makes a lot more sense thanks Nota. :thumbup:


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OfflineSizlChest
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Registered: 09/29/13
Posts: 814
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Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19172517 - 11/21/13 08:26 PM (10 years, 2 months ago)

Quote:

linguafranca said:
Well that was extremely reassuring, thank you. I guess all I can do now is hope that Jeff Goldblum was right in Jurassic Park.





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PrimalSoup's Tea Tek

"I always say the tea is like eating a burning tire covered in dog shit while someone steps on your nuts. Good luck!"
"Hell, shrooms have blown up from under me and kicked my ass on 2 grams once."
"I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."


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Offline2bittoker
Resident PMP Advocate


Registered: 03/09/13
Posts: 555
Last seen: 2 years, 3 months
Re: First timer and extremely nervous about inoculation [Re: SizlChest]
    #19172566 - 11/21/13 08:36 PM (10 years, 2 months ago)

I hope you realize the one in the first picture has a trich infection. The bottom one looks okay


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Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
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OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
Re: First timer and extremely nervous about inoculation [Re: 2bittoker]
    #19172615 - 11/21/13 08:45 PM (10 years, 2 months ago)

Quote:

2bittoker said:
I hope you realize the one in the first picture has a trich infection. The bottom one looks okay





:ninjawe:

I think hes right.

Could be it some sort of visual illusion, is there
hard water buildup on the bottom half of the jar.

Before you know for sure, I'd isolate that jar, and any
that look like it.

Take a picture in decent lighting, or with flash, and
post right away.

Pronto!


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The ego is nothing other than the focus of conscious attention.


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OfflineDannyDGAF
Boomer Enthusiast
Male User Gallery


Registered: 06/10/13
Posts: 533
Loc: USA Flag
Last seen: 6 years, 8 months
Re: First timer and extremely nervous about inoculation [Re: JMcDoogle]
    #19172653 - 11/21/13 08:52 PM (10 years, 2 months ago)

I had something similar that looked like that from the steam sterilization process.  It was only on the outside and was fully cleanable with some alcohol.  Hard to tell from the pic tho if its the hard water buildup on the outside or an actual contamination.


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"You tell him, and I will smack you. I will smack you like a bad, bad donkey!"


Our dreams are a second life. I have never been able to penetrate without a shudder those ivory or horned gates which separate us from the invisible world.


"In order to use your head, you have to go out of your mind" -  Tim Leary


Edited by DannyDGAF (11/21/13 08:53 PM)


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Offlinelinguafranca
Virgin cultivator
Male


Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: DannyDGAF]
    #19173200 - 11/22/13 12:16 AM (10 years, 2 months ago)

I took the jar out of the incubator and set it away in a corner of my closet that's dark and isolated. Here's a link to some pictures I took in better lighting


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
Trusted Cultivator
Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19173205 - 11/22/13 12:18 AM (10 years, 2 months ago)

Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.


Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR


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Offlinelinguafranca
Virgin cultivator
Male


Registered: 10/30/13
Posts: 16
Last seen: 10 years, 1 month
Re: First timer and extremely nervous about inoculation [Re: PussyFart]
    #19173218 - 11/22/13 12:22 AM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.


Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years.  Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger.
RR




thanks man. I had no clue!


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Edited by linguafranca (11/22/13 12:23 AM)


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Offlines0mer4ndom1diot
Stranger


Registered: 04/20/09
Posts: 93
Last seen: 2 months, 19 days
Re: First timer and extremely nervous about inoculation [Re: linguafranca]
    #19173509 - 11/22/13 03:09 AM (10 years, 2 months ago)

appears to have been an illusion. that jar looks perfectly fine to me from those better pics.


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OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
Re: First timer and extremely nervous about inoculation [Re: s0mer4ndom1diot]
    #19173521 - 11/22/13 03:14 AM (10 years, 2 months ago)

Quote:

s0mer4ndom1diot said:
appears to have been an illusion. that jar looks perfectly fine to me from those better pics.




Its because he did not post a picture
of the jar in question silly...

You should be more observant -

He posted new pictures of a jar that is fully
colonized.

The jar with the weird green growth is only partial colonized
near the top.


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