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InvisiblebodhisattaMDiscordReddit
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Time to do some work AGAR edition
    #19057195 - 10/30/13 11:53 AM (10 years, 3 months ago)

Here's 6 dishes. 3 each AA+ and Creeper.



Obviously some of these dishes have a couple contam spots and one dish is really fucked up.

I'm expanding each of these dishes to 3-4 more dishes. I chose some dishes with contams on them just to practice my agar technique in a SAB and see if I am any good at cleaning up cultures.

Most of these dishes are from clone tissue but a couple are from a wedge transfer of the best myc from a original dish that was made with spores IE (limited MS).

I'm hoping to work my way to isolates by taking very small slivers from these dishes. I imagine one more transfer after this set of transfers should get me to an isolate.

I'm not going to let these dishes just serve once purpose though as well as the set I'm making up right now. Both of these sets of dishes I'm going to use the myc I decided not to transfer to new dishes to grow out to grain jars while I do my AGAR project.

Since I'm only growing for my own amusement and :aweyeah: for the wife and I have a feeling I'm going to go about my cultivation hobby like this from now on.

Sporeprint > agar > isolate (2-3transfers?) but use wedges from dishes along the way to innoc quart grain jars. I'm doing shoebox bricks(see signature) that use 2q spawn and 2q sub so I don't need to bother with G2G expansion. > by the time I get to an isolates I'll use those to inoc more quart jars. and the first set of limited MS will be fruiting or close to it. I'll clone fruits from the limited MS grow and then work those to isolates. I'll also take prints and start the whole process over again to avoid senility:lol: in my cultures and keep practicing the hobby. Sounds like more fun than work for me.

If I do this for a few years and always go clone/isolate and spore print from those and then start a new clone/isolate project with spore prints from clone/isolate fruits would my genetics diverge to a unique "variety" after years?


Edited by Trusted cuItivator (10/30/13 11:53 AM)


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Invisible36fuckin5
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057240 - 10/30/13 12:00 PM (10 years, 3 months ago)

Those don't look like they're wrapped with anything. You should have Parafilm or at least plastic cling wrap on them.


--------------------
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If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19057277 - 10/30/13 12:05 PM (10 years, 3 months ago)

Quote:

36fuckin5 said:
Those don't look like they're wrapped with anything. You should have Parafilm or at least plastic cling wrap on them.




I know. Plastic wrap is a pain in the dick to use. I actually keep them in tin foil since I PC my glass petris in there. I had to take them out of the foil to get a picture but I don't think I fucked them up in this short time. They're loaded in the SAB right now waiting for everything to settle.

I actually think most of the contams on these dishes came from handling them so much and watching them from day to day taking them out of the foil and what not. I only payed so much attention to them because it's my first couple times with agar. From now on I'm just letting them sit wrapped up until I'm ready and they're ready based on time.

I have a stack of dishes wrapped in foil. I open them up and pour from the bottom dish upwards and then put the foil back around. Then I come back unwrap and put my samples into each dish from the bottom up and then re-wrap. The foil is folded nicely so it's not some crumpled up ball all around it and it stays relatively nice. I usually do them 6-8 high. I don't get much of a condensation problem doing it this way.

I'm planning on moving to plastic petri dishes and am actually thinking about using PP containers ala Violets newest thread.


Edited by Trusted cuItivator (10/30/13 12:07 PM)


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Invisible36fuckin5
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057316 - 10/30/13 12:13 PM (10 years, 3 months ago)

They really need to be wrapped in something. Foil isn't gonna cut it. That's probably why you're having so many contams, and you'll continue to until you start wrapping your plates.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19057348 - 10/30/13 12:21 PM (10 years, 3 months ago)

:whathesaid:


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19057400 - 10/30/13 12:31 PM (10 years, 3 months ago)

Noted.

I'll buy some name brand saran wrap that one at least kind of works well for me.

I hate cling wrap products. Maybe I fail at life but I always fail hard using that shit. It either wont tear or get stuck to itself or it will decide not to stick to itself even when stretched or some other shit.


Edited by Trusted cuItivator (10/30/13 12:33 PM)


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057435 - 10/30/13 12:40 PM (10 years, 3 months ago)

I use parafilm. Works great. I've never even tried anything else.

I often store my poured dishes in a new ziplock. I only open the ziplock in my SAB. But, a contaminated plate may contaminate your other plates, so use separate bags if you go this route.


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Re: Time to do some work AGAR edition [Re: bodhisatta] * 1
    #19057444 - 10/30/13 12:42 PM (10 years, 3 months ago)

Just bite the bullet and get some parafilm. If you are going to take up that much time and energy to get it right, don't skimp on something that could wreck your whole project.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19057452 - 10/30/13 12:44 PM (10 years, 3 months ago)

Quote:

SpitballJedi said:
I use parafilm. Works great. I've never even tried anything else.

I often store my poured dishes in a new ziplock. I only open the ziplock in my SAB. But, a contaminated plate may contaminate your other plates, so use separate bags if you go this route.




I do know of parafilm it's just not something I wanted to invest in right now. I know flame away.

I like the ziplock idea I just wish they made one a perfect size for one dish so it wouldn't slide around either.


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057485 - 10/30/13 12:52 PM (10 years, 3 months ago)

Just saying what I do. I wanted to express my experience so you wouldn't think I was talking out of my ass. Didn't intend for it to come across as flaming.

The ziplocks seem wasteful, but they are easy. I put 4 petris in a quart double lock freezer bag and can fit 5-6 of those in a gallon bag.

I've had some unwrapped agar plates in storage this way for about 9 months and they have no contams.

Sliding around doesn't seem to be a big deal. Just pay attention to what you're doing.

Just food for thought.

Good luck to you. You are a good member.


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Edited by SpitballJedi (10/30/13 12:52 PM)


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Re: Time to do some work AGAR edition [Re: bootster]
    #19057499 - 10/30/13 12:54 PM (10 years, 3 months ago)

You should have something like this when you're done:



Each Petri Dish is wrapped in Parafilm and in it's own baggie.


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Re: Time to do some work AGAR edition [Re: SpitballJedi] * 1
    #19057500 - 10/30/13 12:55 PM (10 years, 3 months ago)

I didn't think you were I just thought I would take flac for not wanting to buy parafilm lol. Shit's expensive. I didn't think the foil was the cause of my problem but now I'm going to play around with cling wrap and ziplocks since I have both of them and maybe buy parafilm if I have some extra money in the future.

I hate that I have so little space. Two people in 600SqFt is outrageous especially when one has more clothes and shoes than I'll ever own in my whole life just for one season of the year.

Then there's a cat who's very interested in polyfil and the misses doesn't want 10 tubs full of myco supplies laying around.

I wear the pants just enough to dictate that the fan does not go on in the room with the SGFC. Which also is an "eye sore"


Edited by Trusted cuItivator (10/30/13 12:59 PM)


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057511 - 10/30/13 12:56 PM (10 years, 3 months ago)

i love how there is no condensation. is this typical for glass petris? i had a problem with condensation this last time, which was my first time.

when i did my last agar i:
1) know i didnt wait long enough to cool the agar properly.
2) didnt stack them together for long enough as i was too eager to put some spores down
3) i used MS syringe solution that contains too much water already.

im ready to start some more tonight. i think ill make the petris and keep them in their sleeve for a day or two at room temp this time, per advice i saw RR give on an old thread.

im still going to try a couple various ways to use MS syringe solution on them but i am definitely going to use prints for most of the plates.


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Re: Time to do some work AGAR edition [Re: poopy mcpooperson]
    #19057530 - 10/30/13 01:01 PM (10 years, 3 months ago)

To get spores on agar flame your inoculation loop and then squirt 2-3 drops onto the loop the first one or two will cool it down then you can smear just a single drip onto the plate to keep the plates as dry as possible. Or if you're really good at squirting out a single drip you could do that too.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057538 - 10/30/13 01:04 PM (10 years, 3 months ago)

I think agar has it's own gremlins. Sometimes you just know you got it right and it's all f*cked up, and then, when you think you've screwed it all up, it comes out nice.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057562 - 10/30/13 01:09 PM (10 years, 3 months ago)

Yeah, I feel ya. My ex-wife used to let me wear pants sometimes.

Parafilm is nice, but it's not as critical as some people make it out to be. The important thing is to keep those dishes sterile.


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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19057832 - 10/30/13 02:09 PM (10 years, 3 months ago)

of course,
no need for pesty ,easily contamed and broken glass dishes,
or parafilm.......



behold, the polycarbonate culture bottle.

not a bad little agar garden bod,
i see potential.


--------------------
:aliendance:


Edited by anne halonium (10/30/13 02:09 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: anne halonium] * 1
    #19065453 - 10/31/13 07:23 PM (10 years, 2 months ago)

Any one ever eat agar. I'm thinking the malt extract agar once jelled wouldn't be so bad. Maybe add some hops to it.

Agar beer snacks sort of like fruit snacks


Edited by Trusted cuItivator (10/31/13 07:24 PM)


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OfflineStromriderM
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19065464 - 10/31/13 07:25 PM (10 years, 2 months ago)

Quote:

bodhisatta said:
Any one ever eat agar. I'm thinking the malt extract agar once jelled wouldn't be so bad. Maybe add some hops to it.

Agar beer snacks sort of like fruit snacks




:smbfacepalm: :rofl:


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19065477 - 10/31/13 07:28 PM (10 years, 2 months ago)

i don't use parafilm either, shit IS expensive and i'm a cheapass.

i just wrap each dish in saran wrap (which I also hate because it sticks to itself and i've cut my finger open multiple times on the box trying to pull the saran wrap out!)  then take my individually wrapped dishes and put em all in a big ziplock freezer bag.  no problems.

if u have the money to burn, i'd get parafilm because it supposedly is the best thing to use, but i don't have any issues using saran wrap and ziplocks :shrug:

lookin good though man, nice work :thumbup:


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Re: Time to do some work AGAR edition [Re: PsiLisaBin]
    #19065493 - 10/31/13 07:31 PM (10 years, 2 months ago)

Just poured 10 more dishes. I know I fucked one up good though agar dripped onto the edge of the bottom plate so that one is fucked. I think I'm going to chance it and transfer to the dishes as soon as the solidify and cool enough.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19100888 - 11/07/13 02:25 PM (10 years, 2 months ago)

Well I'm almost embarased to upload this because I wrapped the dishes with holiday saran wrap.

I'll be getting clear stuff but I didn't want to go to the store just to get some work done.



The one test plate is still clean. I scraped the middle with my scalpel after I flamed it just to make sure I'm sterile as well as the dish.

The bottom left plate has one spot of bacteria on it.

These were the best looking of the new plates I made. Some of the others were destroyed and resteralized to make new dishes to transfer wedges from this batch onto.

How many more transfers to get an isolate you think?


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19100913 - 11/07/13 02:29 PM (10 years, 2 months ago)

Lolol, you're not supposed to cover the whole thing in Saran wrap, just the edges. The same way you'd do with Parafilm. I usually cut about a 1" roll off and use that.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19100968 - 11/07/13 02:38 PM (10 years, 2 months ago)

:whathesaid::lmafo:


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19101009 - 11/07/13 02:45 PM (10 years, 2 months ago)

Personally, I think your Barbie Dream Agar KIT is just swell. It would make a nice addition to Notahacker420's Barbie Dream SGFC.

But, I see at least 2-4 sectors in each plate. It's hard to say how many it will take. I would estimate 2 more from each of those sectors. But, that's just a guess.


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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19101095 - 11/07/13 02:57 PM (10 years, 2 months ago)

That thing worked like a champ, that's all I got to say.




lol


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Re: Time to do some work AGAR edition [Re: PussyFart]
    #19101166 - 11/07/13 03:07 PM (10 years, 2 months ago)

Pretty AND smart. :thumbup:


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19103157 - 11/07/13 10:08 PM (10 years, 2 months ago)

I know how the parafilm works but saran is a dick to work with especially in a SAB mine's not the same size as NaH's but it's taller. I wrap them inside the SAB so the 1 inch thing is a pain in the dick. I really should just buy parafilm. Hence my embarrassment uploading these. At least my contam rate went down.


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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19103162 - 11/07/13 10:09 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
Personally, I think your Barbie Dream Agar KIT is just swell. It would make a nice addition to Notahacker420's Barbie Dream SGFC.

But, I see at least 2-4 sectors in each plate. It's hard to say how many it will take. I would estimate 2 more from each of those sectors. But, that's just a guess.




That's quite a nice SGFC trades?! haha.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19105087 - 11/08/13 11:17 AM (10 years, 2 months ago)

Quote:

bodhisatta said:I wrap them inside the SAB so the 1 inch thing is a pain in the dick.




Wut? No it's not. It's much easier to maneuver a little 1" section from that roll as opposed to the whole damn thing inside your SAB.

You realize I meant to cut the WHOLE roll, right? Lop an inch right off the end and use that.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19105416 - 11/08/13 12:25 PM (10 years, 2 months ago)

Quote:

36fuckin5 said:
Quote:

bodhisatta said:I wrap them inside the SAB so the 1 inch thing is a pain in the dick.




Wut? No it's not. It's much easier to maneuver a little 1" section from that roll as opposed to the whole damn thing inside your SAB.

You realize I meant to cut the WHOLE roll, right? Lop an inch right off the end and use that.




although i dont use parafilm, if i did,
36-f-5 has the right idea.


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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19105907 - 11/08/13 02:14 PM (10 years, 2 months ago)

Quote:

36fuckin5 said:
Quote:

bodhisatta said:I wrap them inside the SAB so the 1 inch thing is a pain in the dick.




Wut? No it's not. It's much easier to maneuver a little 1" section from that roll as opposed to the whole damn thing inside your SAB.

You realize I meant to cut the WHOLE roll, right? Lop an inch right off the end and use that.




It crossed my mind but I don't have any cutting utensils that are not going to mangle the shit out of the wrap if I try that. I could probably use a razor blade and just hack through it though. My kitchen knives are shit, and I don't have any saws.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19110546 - 11/09/13 02:06 PM (10 years, 2 months ago)

Well I got clear wrap today. Going to try the 1 inch thing out. Just poured 15 plates with a much smoother procedure than usual. I took only 5 of the pink plates from the above post and am expanding them to 3. I also took the liberty of inoculating 5 quarts with wedges from the above plates. I threw out the 6th plate because green showed up on the contam spot that was the bottom left dish in the picture. Pictures in a couple hours after I get all of this shit done.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19110970 - 11/09/13 04:00 PM (10 years, 2 months ago)

I have a good feeling
:holyshitbubbles:


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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19112146 - 11/09/13 09:12 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
I have a good feeling
:holyshitbubbles:




So do I. I'll take pictures of the new dishes in a couple days when they grow out. They're boring right now


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19114224 - 11/10/13 10:43 AM (10 years, 2 months ago)

ill watch this one.

oddly, im not against the pink shrink wrap.

there are easier ways though.



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Re: Time to do some work AGAR edition [Re: anne halonium]
    #19130846 - 11/13/13 11:37 AM (10 years, 2 months ago)


12345(original dishes)
a (1st xfer)
b (2nd)
c (3rd)






Well some contamination showed up on the No2 and No5 original dishes.

Dish 5b and 2c also show some spots.

Not to bad I guess.

Question
If a contam sporulates on a dish is the whole thing garbage? I would imagine the spores are everywhere inside of the dish or does taking a small section still over run the contams on a new dish.


Edited by Trusted cuItivator (11/13/13 11:38 AM)


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19130873 - 11/13/13 11:46 AM (10 years, 2 months ago)

yes and no, sometimes ya can make a save.


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Re: Time to do some work AGAR edition [Re: anne halonium]
    #19131090 - 11/13/13 12:41 PM (10 years, 2 months ago)

If a contam sporulates on a dish, work in a SAB because the flowhood would blow all the spores on you and your work surface


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19131749 - 11/13/13 03:18 PM (10 years, 2 months ago)

My understanding is the inside of the dish is very still and the spores don't get blown around.

Transferring to a new dish helps to verify.


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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19132108 - 11/13/13 04:47 PM (10 years, 2 months ago)

Good to know. I'll do xfers off of the contamed dishes to a new clean dish in a separate SAB work day.

I have no idea how close I am to isolates still. I don't see sectoring as much in person as it is apparent in the videos.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19132212 - 11/13/13 05:18 PM (10 years, 2 months ago)

sometimes it takes a couple of transfers to see sectoring. Multiple spores germinating and growing at s similar rate can prevent visible sectoring.


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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19132978 - 11/13/13 07:45 PM (10 years, 2 months ago)

hell yeah. thats good to know. on some of my PE prints that i put to agar and some of my 1st transfers im still seeing fluffy myc. i was getting worried.

on a couple of my initial agar spore placement of another print i got 100% rhizomorphic growth and it doesn't look like there is any sectoring. im still going to make transfers just to be sure.


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Re: Time to do some work AGAR edition [Re: poopy mcpooperson]
    #19134660 - 11/14/13 03:31 AM (10 years, 2 months ago)

bodhi, I have same problem with wrapping plates in an SAB, even with parafilm.  I'm still new with agar..only been working with it a month now, but After Im done transferring/cloning/whatever in my SAB, I push all the dishes to one side and other supplies to the opposite.  Pull my gloved hands out of the holes, and gently/slowly lift the lid of the SAB and wrap the plates outside the Box.  probably not a thing many people will endorse, but it has worked for me.  I also have my SAB in a particularly clean room, but by no means is it anything  un accomplishable.  Just the cleanest, uncarpeted room in the house, sprayed lysol and wiped nearest objects with 10% bleach, especially lid of the SAB on the outside, thats where my parafilm or saran is, and I wrap there.  It seems like ur dishes had good luck without ANY wrap in the ambient environment of ur apt, so I suppose ur house/apt is clean enough to accomplish this out of the SAB wrap.  Its just easier, and have had same contam rate as when I did it IN the SAB.







hope that helps, and great thread idea, if i understand it right...is it going to be an agar grow along/agar work along?  If so I'm gonna be hooked!


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Re: Time to do some work AGAR edition [Re: blindingleaf]
    #19135412 - 11/14/13 09:47 AM (10 years, 2 months ago)

Quote:

blindingleaf said:



hope that helps, and great thread idea, if i understand it right...is it going to be an agar grow along/agar work along?  If so I'm gonna be hooked!




That last dish looks awesome. And yea I plan to log the adventure as it goes along.

Most of these dishes were from clone tissue origin but I hear that can also have 100's of genetics.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19139977 - 11/15/13 08:09 AM (10 years, 2 months ago)

I just recently started working with agar I did some prints grain and a clone.  I decided to take a break and work on just isolating so I'm gonna join along


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19140005 - 11/15/13 08:21 AM (10 years, 2 months ago)

Quote:

blueconfusion said:
I just recently started working with agar I did some prints grain and a clone.  I decided to take a break and work on just isolating so I'm gonna join along



:thumbup:

welcome aboard. The more pictures the merrier.

I'm actually trying to slow down how much fruiting I'm doing since I can't realistically eat all the shrooms I make without ending up in a mental institute.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19140031 - 11/15/13 08:31 AM (10 years, 2 months ago)

Yeah I needed to slow down cause my PE MS tubs yeilded a little over 3/4 lbs!!!


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19140043 - 11/15/13 08:34 AM (10 years, 2 months ago)

Quote:

blueconfusion said:
Yeah I needed to slow down cause my PE MS tubs yeilded a little over 3/4 lbs!!!




eat them till you're full.
:dancingbacon:
Makes me want to make a Whats the most you ever ate thread. But people are just going to lie anyway.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19141515 - 11/15/13 04:17 PM (10 years, 2 months ago)

i've eaten 5 grams of azures...  that was pretty incredible and i don't know how many fresh cyans, but never more than 5 grams of cubes :shrug:


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19141565 - 11/15/13 04:30 PM (10 years, 2 months ago)

yea I think most I ever ate was 6g cubes, mckenna style (dark room, silence, alone).  It's my favorite way to do it, ur a different person the next few days if u pull thru. I can't wait to grow something other than cubes!  Just now perfecting them, so its only a matter of time!  Damn, 5 g azures, I can't imagine!


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Re: Time to do some work AGAR edition [Re: blindingleaf]
    #19141677 - 11/15/13 04:55 PM (10 years, 2 months ago)

With the 5 cubes it was just that a dark room in silence.  The azures I started out starting at the stars watching them form pictures then I crawled in my cabin and disappeared in the blankets a truly mind blowing experience


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19142478 - 11/15/13 08:01 PM (10 years, 2 months ago)

I hate 14g of cubes that were made into pills. Pretty much no moving for a few hours.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19144708 - 11/16/13 10:09 AM (10 years, 2 months ago)

Getting ready to pour some PDYA later today I have some bacteria growth on one of my PE clone plates don't want to loose it so here we go


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19144794 - 11/16/13 10:35 AM (10 years, 2 months ago)

Looks like it came in through that gap in your wrap. That happens to me when my parafilm cracks. I think I stretch it too far.


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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19144843 - 11/16/13 10:50 AM (10 years, 2 months ago)

Yep that was what I thought need to be more careful next (this) time :shrug:


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19145569 - 11/16/13 02:22 PM (10 years, 2 months ago)

was able to take 5 good transfers, nothing even near the contamination.  made sure to wrap them well this time, and shut the fan off that was on in the room...  so I'm hoping for the best so i have lots more pics to share of really good sectoring mycelium.


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19145613 - 11/16/13 02:31 PM (10 years, 2 months ago)

Doing some grain transfers tonight as well anything leftover stray grains are going in plates!


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Re: Time to do some work AGAR edition [Re: blueconfusion]
    #19149458 - 11/17/13 12:10 PM (10 years, 2 months ago)

I noticed I had better luck wrapping my dishes like I did the first time around with the pink wrap. I'm just going to do that with the clear stuff from now on rather then just around the edge. Whatever works you know.








Here's the survivors which haven't been overran with contamination.


Edited by Trusted cuItivator (11/17/13 12:14 PM)


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19149654 - 11/17/13 12:57 PM (10 years, 2 months ago)

Quote:

bodhisatta said:
Any one ever eat agar. I'm thinking the malt extract agar once jelled wouldn't be so bad. Maybe add some hops to it.

Agar beer snacks sort of like fruit snacks




Yup, I made some pomegranate agar jellies once.  It's got an interesting texture, for sure.  It's like gelatin, but it shatters rather than cleaving on a plane like gelatin does.  Beer might be fun--when I worked in a lab I always wanted to make jello shots with the agar, DI water, scientific grade ethanol, glucose and citric acid we had, but everyone else was a coward.


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Re: Time to do some work AGAR edition [Re: Psilicon]
    #19149657 - 11/17/13 12:58 PM (10 years, 2 months ago)

Quote:

van der griegen said:
Quote:

bodhisatta said:
Any one ever eat agar. I'm thinking the malt extract agar once jelled wouldn't be so bad. Maybe add some hops to it.

Agar beer snacks sort of like fruit snacks




Yup, I made some pomegranate agar jellies once.  It's got an interesting texture, for sure.  It's like gelatin, but it shatters rather than cleaving on a plane like gelatin does.  Beer might be fun--when I worked in a lab I always wanted to make jello shots with the agar, DI water, scientific grade ethanol, glucose and citric acid we had, but everyone else was a coward.




I would have if I worked there :shrug: pansies..


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19150360 - 11/17/13 03:44 PM (10 years, 2 months ago)

:popcorn:


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