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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: PsiLisaBin]
    #19065493 - 10/31/13 07:31 PM (10 years, 2 months ago)

Just poured 10 more dishes. I know I fucked one up good though agar dripped onto the edge of the bottom plate so that one is fucked. I think I'm going to chance it and transfer to the dishes as soon as the solidify and cool enough.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19100888 - 11/07/13 02:25 PM (10 years, 2 months ago)

Well I'm almost embarased to upload this because I wrapped the dishes with holiday saran wrap.

I'll be getting clear stuff but I didn't want to go to the store just to get some work done.



The one test plate is still clean. I scraped the middle with my scalpel after I flamed it just to make sure I'm sterile as well as the dish.

The bottom left plate has one spot of bacteria on it.

These were the best looking of the new plates I made. Some of the others were destroyed and resteralized to make new dishes to transfer wedges from this batch onto.

How many more transfers to get an isolate you think?


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Invisible36fuckin5
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19100913 - 11/07/13 02:29 PM (10 years, 2 months ago)

Lolol, you're not supposed to cover the whole thing in Saran wrap, just the edges. The same way you'd do with Parafilm. I usually cut about a 1" roll off and use that.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePussyFart
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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19100968 - 11/07/13 02:38 PM (10 years, 2 months ago)

:whathesaid::lmafo:


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19101009 - 11/07/13 02:45 PM (10 years, 2 months ago)

Personally, I think your Barbie Dream Agar KIT is just swell. It would make a nice addition to Notahacker420's Barbie Dream SGFC.

But, I see at least 2-4 sectors in each plate. It's hard to say how many it will take. I would estimate 2 more from each of those sectors. But, that's just a guess.


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OfflinePussyFart
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19101095 - 11/07/13 02:57 PM (10 years, 2 months ago)

That thing worked like a champ, that's all I got to say.




lol


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: PussyFart]
    #19101166 - 11/07/13 03:07 PM (10 years, 2 months ago)

Pretty AND smart. :thumbup:


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19103157 - 11/07/13 10:08 PM (10 years, 2 months ago)

I know how the parafilm works but saran is a dick to work with especially in a SAB mine's not the same size as NaH's but it's taller. I wrap them inside the SAB so the 1 inch thing is a pain in the dick. I really should just buy parafilm. Hence my embarrassment uploading these. At least my contam rate went down.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19103162 - 11/07/13 10:09 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
Personally, I think your Barbie Dream Agar KIT is just swell. It would make a nice addition to Notahacker420's Barbie Dream SGFC.

But, I see at least 2-4 sectors in each plate. It's hard to say how many it will take. I would estimate 2 more from each of those sectors. But, that's just a guess.




That's quite a nice SGFC trades?! haha.


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Invisible36fuckin5
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19105087 - 11/08/13 11:17 AM (10 years, 2 months ago)

Quote:

bodhisatta said:I wrap them inside the SAB so the 1 inch thing is a pain in the dick.




Wut? No it's not. It's much easier to maneuver a little 1" section from that roll as opposed to the whole damn thing inside your SAB.

You realize I meant to cut the WHOLE roll, right? Lop an inch right off the end and use that.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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Invisibleanne halonium
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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19105416 - 11/08/13 12:25 PM (10 years, 2 months ago)

Quote:

36fuckin5 said:
Quote:

bodhisatta said:I wrap them inside the SAB so the 1 inch thing is a pain in the dick.




Wut? No it's not. It's much easier to maneuver a little 1" section from that roll as opposed to the whole damn thing inside your SAB.

You realize I meant to cut the WHOLE roll, right? Lop an inch right off the end and use that.




although i dont use parafilm, if i did,
36-f-5 has the right idea.


--------------------
:aliendance:


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19105907 - 11/08/13 02:14 PM (10 years, 2 months ago)

Quote:

36fuckin5 said:
Quote:

bodhisatta said:I wrap them inside the SAB so the 1 inch thing is a pain in the dick.




Wut? No it's not. It's much easier to maneuver a little 1" section from that roll as opposed to the whole damn thing inside your SAB.

You realize I meant to cut the WHOLE roll, right? Lop an inch right off the end and use that.




It crossed my mind but I don't have any cutting utensils that are not going to mangle the shit out of the wrap if I try that. I could probably use a razor blade and just hack through it though. My kitchen knives are shit, and I don't have any saws.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19110546 - 11/09/13 02:06 PM (10 years, 2 months ago)

Well I got clear wrap today. Going to try the 1 inch thing out. Just poured 15 plates with a much smoother procedure than usual. I took only 5 of the pink plates from the above post and am expanding them to 3. I also took the liberty of inoculating 5 quarts with wedges from the above plates. I threw out the 6th plate because green showed up on the contam spot that was the bottom left dish in the picture. Pictures in a couple hours after I get all of this shit done.


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19110970 - 11/09/13 04:00 PM (10 years, 2 months ago)

I have a good feeling
:holyshitbubbles:


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19112146 - 11/09/13 09:12 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
I have a good feeling
:holyshitbubbles:




So do I. I'll take pictures of the new dishes in a couple days when they grow out. They're boring right now


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Invisibleanne halonium
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19114224 - 11/10/13 10:43 AM (10 years, 2 months ago)

ill watch this one.

oddly, im not against the pink shrink wrap.

there are easier ways though.



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:aliendance:


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: anne halonium]
    #19130846 - 11/13/13 11:37 AM (10 years, 2 months ago)


12345(original dishes)
a (1st xfer)
b (2nd)
c (3rd)






Well some contamination showed up on the No2 and No5 original dishes.

Dish 5b and 2c also show some spots.

Not to bad I guess.

Question
If a contam sporulates on a dish is the whole thing garbage? I would imagine the spores are everywhere inside of the dish or does taking a small section still over run the contams on a new dish.


Edited by Trusted cuItivator (11/13/13 11:38 AM)


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Invisibleanne halonium
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19130873 - 11/13/13 11:46 AM (10 years, 2 months ago)

yes and no, sometimes ya can make a save.


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OfflineeLeSDenes
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Re: Time to do some work AGAR edition [Re: anne halonium]
    #19131090 - 11/13/13 12:41 PM (10 years, 2 months ago)

If a contam sporulates on a dish, work in a SAB because the flowhood would blow all the spores on you and your work surface


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19131749 - 11/13/13 03:18 PM (10 years, 2 months ago)

My understanding is the inside of the dish is very still and the spores don't get blown around.

Transferring to a new dish helps to verify.


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