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InvisiblebodhisattaMDiscordReddit
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Time to do some work AGAR edition
    #19057195 - 10/30/13 11:53 AM (10 years, 2 months ago)

Here's 6 dishes. 3 each AA+ and Creeper.



Obviously some of these dishes have a couple contam spots and one dish is really fucked up.

I'm expanding each of these dishes to 3-4 more dishes. I chose some dishes with contams on them just to practice my agar technique in a SAB and see if I am any good at cleaning up cultures.

Most of these dishes are from clone tissue but a couple are from a wedge transfer of the best myc from a original dish that was made with spores IE (limited MS).

I'm hoping to work my way to isolates by taking very small slivers from these dishes. I imagine one more transfer after this set of transfers should get me to an isolate.

I'm not going to let these dishes just serve once purpose though as well as the set I'm making up right now. Both of these sets of dishes I'm going to use the myc I decided not to transfer to new dishes to grow out to grain jars while I do my AGAR project.

Since I'm only growing for my own amusement and :aweyeah: for the wife and I have a feeling I'm going to go about my cultivation hobby like this from now on.

Sporeprint > agar > isolate (2-3transfers?) but use wedges from dishes along the way to innoc quart grain jars. I'm doing shoebox bricks(see signature) that use 2q spawn and 2q sub so I don't need to bother with G2G expansion. > by the time I get to an isolates I'll use those to inoc more quart jars. and the first set of limited MS will be fruiting or close to it. I'll clone fruits from the limited MS grow and then work those to isolates. I'll also take prints and start the whole process over again to avoid senility:lol: in my cultures and keep practicing the hobby. Sounds like more fun than work for me.

If I do this for a few years and always go clone/isolate and spore print from those and then start a new clone/isolate project with spore prints from clone/isolate fruits would my genetics diverge to a unique "variety" after years?


Edited by Trusted cuItivator (10/30/13 11:53 AM)


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Invisible36fuckin5
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057240 - 10/30/13 12:00 PM (10 years, 2 months ago)

Those don't look like they're wrapped with anything. You should have Parafilm or at least plastic cling wrap on them.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19057277 - 10/30/13 12:05 PM (10 years, 2 months ago)

Quote:

36fuckin5 said:
Those don't look like they're wrapped with anything. You should have Parafilm or at least plastic cling wrap on them.




I know. Plastic wrap is a pain in the dick to use. I actually keep them in tin foil since I PC my glass petris in there. I had to take them out of the foil to get a picture but I don't think I fucked them up in this short time. They're loaded in the SAB right now waiting for everything to settle.

I actually think most of the contams on these dishes came from handling them so much and watching them from day to day taking them out of the foil and what not. I only payed so much attention to them because it's my first couple times with agar. From now on I'm just letting them sit wrapped up until I'm ready and they're ready based on time.

I have a stack of dishes wrapped in foil. I open them up and pour from the bottom dish upwards and then put the foil back around. Then I come back unwrap and put my samples into each dish from the bottom up and then re-wrap. The foil is folded nicely so it's not some crumpled up ball all around it and it stays relatively nice. I usually do them 6-8 high. I don't get much of a condensation problem doing it this way.

I'm planning on moving to plastic petri dishes and am actually thinking about using PP containers ala Violets newest thread.


Edited by Trusted cuItivator (10/30/13 12:07 PM)


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Invisible36fuckin5
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057316 - 10/30/13 12:13 PM (10 years, 2 months ago)

They really need to be wrapped in something. Foil isn't gonna cut it. That's probably why you're having so many contams, and you'll continue to until you start wrapping your plates.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: 36fuckin5]
    #19057348 - 10/30/13 12:21 PM (10 years, 2 months ago)

:whathesaid:


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19057400 - 10/30/13 12:31 PM (10 years, 2 months ago)

Noted.

I'll buy some name brand saran wrap that one at least kind of works well for me.

I hate cling wrap products. Maybe I fail at life but I always fail hard using that shit. It either wont tear or get stuck to itself or it will decide not to stick to itself even when stretched or some other shit.


Edited by Trusted cuItivator (10/30/13 12:33 PM)


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057435 - 10/30/13 12:40 PM (10 years, 2 months ago)

I use parafilm. Works great. I've never even tried anything else.

I often store my poured dishes in a new ziplock. I only open the ziplock in my SAB. But, a contaminated plate may contaminate your other plates, so use separate bags if you go this route.


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Invisiblebootster
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Re: Time to do some work AGAR edition [Re: bodhisatta] * 1
    #19057444 - 10/30/13 12:42 PM (10 years, 2 months ago)

Just bite the bullet and get some parafilm. If you are going to take up that much time and energy to get it right, don't skimp on something that could wreck your whole project.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19057452 - 10/30/13 12:44 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
I use parafilm. Works great. I've never even tried anything else.

I often store my poured dishes in a new ziplock. I only open the ziplock in my SAB. But, a contaminated plate may contaminate your other plates, so use separate bags if you go this route.




I do know of parafilm it's just not something I wanted to invest in right now. I know flame away.

I like the ziplock idea I just wish they made one a perfect size for one dish so it wouldn't slide around either.


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057485 - 10/30/13 12:52 PM (10 years, 2 months ago)

Just saying what I do. I wanted to express my experience so you wouldn't think I was talking out of my ass. Didn't intend for it to come across as flaming.

The ziplocks seem wasteful, but they are easy. I put 4 petris in a quart double lock freezer bag and can fit 5-6 of those in a gallon bag.

I've had some unwrapped agar plates in storage this way for about 9 months and they have no contams.

Sliding around doesn't seem to be a big deal. Just pay attention to what you're doing.

Just food for thought.

Good luck to you. You are a good member.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Edited by SpitballJedi (10/30/13 12:52 PM)


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Invisiblebootster
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Re: Time to do some work AGAR edition [Re: bootster]
    #19057499 - 10/30/13 12:54 PM (10 years, 2 months ago)

You should have something like this when you're done:



Each Petri Dish is wrapped in Parafilm and in it's own baggie.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: SpitballJedi] * 1
    #19057500 - 10/30/13 12:55 PM (10 years, 2 months ago)

I didn't think you were I just thought I would take flac for not wanting to buy parafilm lol. Shit's expensive. I didn't think the foil was the cause of my problem but now I'm going to play around with cling wrap and ziplocks since I have both of them and maybe buy parafilm if I have some extra money in the future.

I hate that I have so little space. Two people in 600SqFt is outrageous especially when one has more clothes and shoes than I'll ever own in my whole life just for one season of the year.

Then there's a cat who's very interested in polyfil and the misses doesn't want 10 tubs full of myco supplies laying around.

I wear the pants just enough to dictate that the fan does not go on in the room with the SGFC. Which also is an "eye sore"


Edited by Trusted cuItivator (10/30/13 12:59 PM)


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Invisiblepoopy mcpooperson
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057511 - 10/30/13 12:56 PM (10 years, 2 months ago)

i love how there is no condensation. is this typical for glass petris? i had a problem with condensation this last time, which was my first time.

when i did my last agar i:
1) know i didnt wait long enough to cool the agar properly.
2) didnt stack them together for long enough as i was too eager to put some spores down
3) i used MS syringe solution that contains too much water already.

im ready to start some more tonight. i think ill make the petris and keep them in their sleeve for a day or two at room temp this time, per advice i saw RR give on an old thread.

im still going to try a couple various ways to use MS syringe solution on them but i am definitely going to use prints for most of the plates.


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: poopy mcpooperson]
    #19057530 - 10/30/13 01:01 PM (10 years, 2 months ago)

To get spores on agar flame your inoculation loop and then squirt 2-3 drops onto the loop the first one or two will cool it down then you can smear just a single drip onto the plate to keep the plates as dry as possible. Or if you're really good at squirting out a single drip you could do that too.


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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057538 - 10/30/13 01:04 PM (10 years, 2 months ago)

I think agar has it's own gremlins. Sometimes you just know you got it right and it's all f*cked up, and then, when you think you've screwed it all up, it comes out nice.


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InvisibleSpitballJedi
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19057562 - 10/30/13 01:09 PM (10 years, 2 months ago)

Yeah, I feel ya. My ex-wife used to let me wear pants sometimes.

Parafilm is nice, but it's not as critical as some people make it out to be. The important thing is to keep those dishes sterile.


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A little civility goes a long way

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Invisibleanne halonium
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Re: Time to do some work AGAR edition [Re: SpitballJedi]
    #19057832 - 10/30/13 02:09 PM (10 years, 2 months ago)

of course,
no need for pesty ,easily contamed and broken glass dishes,
or parafilm.......



behold, the polycarbonate culture bottle.

not a bad little agar garden bod,
i see potential.


--------------------
:aliendance:


Edited by anne halonium (10/30/13 02:09 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Time to do some work AGAR edition [Re: anne halonium] * 1
    #19065453 - 10/31/13 07:23 PM (10 years, 2 months ago)

Any one ever eat agar. I'm thinking the malt extract agar once jelled wouldn't be so bad. Maybe add some hops to it.

Agar beer snacks sort of like fruit snacks


Edited by Trusted cuItivator (10/31/13 07:24 PM)


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OfflineStromriderM
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19065464 - 10/31/13 07:25 PM (10 years, 2 months ago)

Quote:

bodhisatta said:
Any one ever eat agar. I'm thinking the malt extract agar once jelled wouldn't be so bad. Maybe add some hops to it.

Agar beer snacks sort of like fruit snacks




:smbfacepalm: :rofl:


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InvisiblePsiLisaBin
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Re: Time to do some work AGAR edition [Re: bodhisatta]
    #19065477 - 10/31/13 07:28 PM (10 years, 2 months ago)

i don't use parafilm either, shit IS expensive and i'm a cheapass.

i just wrap each dish in saran wrap (which I also hate because it sticks to itself and i've cut my finger open multiple times on the box trying to pull the saran wrap out!)  then take my individually wrapped dishes and put em all in a big ziplock freezer bag.  no problems.

if u have the money to burn, i'd get parafilm because it supposedly is the best thing to use, but i don't have any issues using saran wrap and ziplocks :shrug:

lookin good though man, nice work :thumbup:


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