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InvisibleNOS4A2
This is the way

Registered: 07/22/04
Posts: 572
Loc: -tite Flag
1st agar
    #19056530 - 10/30/13 07:56 AM (10 years, 3 months ago)

1st agar attempt using homemade PDA.
-20 plates made.
-using vendor B+ spore syringe noc up three plates in large GB.
-other plates look clean.
-within 24hr all three noc'd plates show pink dots which in 3 days become full on pink mold.
-3 plates had good quantity of water ontop of agar some of which came from syringe.
-before noc'ing the cap was hard to pull off and syringe and accidentally was stuck through glove and finger nail into finger.
-Syringe was then rubbed with alcohol and flamed till red and in between each plate.

Could this cause the pink contam?

Or was syringe most likely contaminated from vendor?


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OfflineKizzle
Misanthrope
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Registered: 08/30/11
Posts: 9,855
Last seen: 11 hours, 42 minutes
Trusted Cultivator
Re: 1st agar [Re: NOS4A2]
    #19057404 - 10/30/13 12:32 PM (10 years, 2 months ago)

Could be a contaminated syringe. It is easy to use too much solution which can affect if any mushroom mycelium grows.


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InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: 1st agar [Re: Kizzle]
    #19061819 - 10/31/13 05:19 AM (10 years, 2 months ago)

The agar was kind of gritty as well which suggests the mix needs to be adjusted. Perhaps it was too nutrient rich causing the spores not to grow and the contam to thrive....?
Going to try again and see if same thing happens with an old spore print.
wish me luck


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OfflineKizzle
Misanthrope
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Registered: 08/30/11
Posts: 9,855
Last seen: 11 hours, 42 minutes
Trusted Cultivator
Re: 1st agar [Re: NOS4A2]
    #19063131 - 10/31/13 12:28 PM (10 years, 2 months ago)

Just from there being to much moisture. Spore prints work a lot better on agar.


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