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MyTiny_Shroom
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Registered: 08/30/13
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Shiitake myc. agar?
#19056368 - 10/30/13 06:21 AM (10 years, 3 months ago) |
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Hi, All! I cloned a few shiitake fruit pieces more than 2 weeks ago on agar jars. One quart jar really took off with myc. disbursing 100% in all directions running up the bottom of the jar while the second cloned shiitake jar have myc. spreading evenly outward but in a slower orderly fashion like snowflakes all surrounding the mushroom pieces but still the agar jar has not been completely colonize yet. I would like to clone more into new agar jars. My question is should I clone from the first fast spreading jar or the second more orderly snowflakes looking spreading jar? I'm not sure which jar is better when I put them on sawdust/straw, eventually.
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Amanita virosa
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Registered: 12/04/11
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the "snowflakes" are likely yeast. Always use the fastest growing most vigorous clone you have. yeast looks like this:
here is healthy shiitake myc jar in middle:
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deadmandave
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Registered: 02/16/10
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Only way (imo) to find out which is better would be to try them both and take notes to notice the better performer.
Were these cloned from different mushrooms? where did you get them?
AV, what do you do with your yeasty jars? i have spawned oysters looking like that to straw and it turned out ok
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Amanita virosa
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Spawned. No problem. Impossible to avoid really and I can find no noticable difference in performance. Still, it makes me cringe
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MyTiny_Shroom
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Registered: 08/30/13
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I don't know why one agar jar has yeast while the other does not. Is this part of the normal growth with yeast in them? Then how do I know which one is the shiitake myc. different from the yeast? Humm, I'm really confused now. Is there a way to isolate for the shiitake myc. only? Alright then I will try to clone the bumpy shiitake myc. in one of the jar and the orderly myc. from the other jar. Let's see what happen next.
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Amanita virosa
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Registered: 12/04/11
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Impossible to isolate the yeast from the Myc. Yeast is unicellular but will produce pseudo hyphae on grain and agar. These look like very fat hyphae without cross walls. Don't worry about the yeast. Use the cleaner jar to expand though
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MyTiny_Shroom
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Got it! Thanks much.
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MyTiny_Shroom
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An update: The first jar myc. started browning for some reason but some are still white. If I clone them again on agar, can I use the brown color myc.? Or only use the white myc.?
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Jeff
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Registered: 10/06/12
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Interesting, I have never had shiitake go brown on a plate.
If you can, a picture would be nice.
-------------------- Myco-tek
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Amanita virosa
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Registered: 12/04/11
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Re: Shiitake myc. agar? [Re: Jeff]
#19102537 - 11/07/13 07:53 PM (10 years, 2 months ago) |
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Takes a while usually but does happen ESP if unrefridged. I just opened a plate of shiitake straw today and it had a perfect little shiitake coming off of the wedge right in the middle of the plate. I thought if it fruits on agar it prolly will fruit on straw. Wish I woulda took a picture. It was really cute. Op I think the browner it gets the less viable so if ur subbing it out use the whiter Myc.
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Jeff
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Very cool AV. I have had plenty of things fruit on a plate but shiitake has not been one of them.
-------------------- Myco-tek
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MyTiny_Shroom
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Re: Shiitake myc. agar? [Re: Jeff]
#19103286 - 11/07/13 10:34 PM (10 years, 2 months ago) |
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Shiitake is a good tasty treat. Crunchy and juicy all the way up to the stem. Saute in a bit of soy sauce in a soup base. Yummy!!! I can eat a whole plate and want some more. I'm sure there are many recipes as there are mushrooms out there. A successful farm has 80% product mix in the Shiitake mushrooms.
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cmspice
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I am surprised there is no section on yeast in the main contaminants thread. It seems like it's pretty harmless esp since there hardly any fermentable sugars except at the agar stage. My myc always grows right over the yeast contams like it's no one's business. (short story: I was getting a lot of yeast contams when I was doing MYA and I switched to potato agar (no dextrose) and thought it was weird there were no more yeast contams before realizing there's no food for the yeast). Seems like it might even be desireable for preventing other contams not that I would really know.
-------------------- have: blue, brown, pink, yellow, elm, king oyster, reishi, nameko, black poplar, shaggy mane, PESA want: ABM, ganoderma spp., straw mushroom, exotic pleurotus species, ethno cuttings/seeds.
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Zen Peddler


Registered: 06/18/01
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Loc: orbit
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Quote:
Amanita virosa said: Always use the fastest growing most vigorous clone you have.
A few times ive seen sluggish strains outperform fast runners although not specifically with shiitake.
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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If you have a specimen of a mushroom that looks a little old and you want to clone it you can of course just dip it in h202 and then transfer it to a h202 plate. Not exactly an organic method but it always works for me.
When cloning sometimes you get lucky but as a general rule you will lose a few plates to various contaminants. I have more like with wild print streaks than I have with some clones but sometimes I can do twenty plates and get zero contaminants.
With any clone run at least 10-12 plates.
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Snktido
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Registered: 11/16/13
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Last seen: 9 years, 10 months
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how about into liquid culture? First time making Shitake liquid culture and it doesn't seem to get thick like Oyster mushrooms. I heard that some species produce thick clumps of mycelium and some does not. How is Shitake suppose to perform in LC?
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MyTiny_Shroom
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Re: Shiitake myc. agar? [Re: Snktido]
#19176297 - 11/22/13 07:51 PM (10 years, 2 months ago) |
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I don't know about Shiitake LC performance. My experienced with oyster LC are that they are easily to contaminate when I open up the jars. Perhaps it is with my method of sterilization or handling them. And my syringe needle was too small to take up the myc. on transfer. Too much trouble for me I think. But with agar culture inside the quart jars, I don't have to open them until I'm ready to transfer the myc. for agar expansion. This way I can clone 100 jars or more if I want. Then transfer these agar wedges into seeds substrate for further expansion. It is very simple to make the agar wedges. Both oyster and shiitake grow fast on them and last months inside these jars without any refrigeration. Some shiitake even pins inside the agar jar too. Now I can clone the pins or myc. if I want to. No contamination there. I'm happy with that.
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Snktido
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Registered: 11/16/13
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Last seen: 9 years, 10 months
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thanks for the info, I am definitely going try the agar route and probably use it as the primary means of inoculation. However I want to experiment with liquid culture for when i use hard to colonize substrate like cardboard or make bulk. I have lots of cardboard lying around from all my amazon orders and surely its cheap, free and everywhere in mass quantities. Anyhow, I am starting to get a good idea of how Shitake mycelium grows in LC and how healthy growth should look like. The growth is not as thick and visible as that of oyster genera. It is more fine and loose.
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