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DoSedLiving

Registered: 03/01/09
Posts: 632
Last seen: 5 years, 1 month
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Is there a good way to isolate agar cultures after cobweb mold has sporulated in it?
#19053892 - 10/29/13 07:50 PM (10 years, 3 months ago) |
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JW? I have some cultures, cobweb mold was the first to show up and has since grown black dots at the top, im assuming those are its spores? Can you still isolate myc. from these without too much work? Whats a good method? all I can think is pull out most of the CW mold dip a loop in bleach and try and pull out some myc to transfer..
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: Is there a good way to isolate agar cultures after cobweb mold has sporulated in it? [Re: DoSedLiving]
#19053931 - 10/29/13 07:54 PM (10 years, 3 months ago) |
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If the plate is covered in cobweb mold, it is lost.
If you can catch it before this stage sure it's possible, but it will take over a dish in a matter of 2 days so you better be quick lol.
If there is clean mycellium exposed, transfer it.
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Edited by PussyFart (10/29/13 07:55 PM)
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 15 hours, 37 minutes
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Re: Is there a good way to isolate agar cultures after cobweb mold has sporulated in it? [Re: PussyFart]
#19054303 - 10/29/13 09:11 PM (10 years, 3 months ago) |
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DoSedLiving

Registered: 03/01/09
Posts: 632
Last seen: 5 years, 1 month
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Re: Is there a good way to isolate agar cultures after cobweb mold has sporulated in it? [Re: rumfor69]
#19054379 - 10/29/13 09:27 PM (10 years, 3 months ago) |
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Fk, the problem is is CW was the first to show up, it was hours from sporulating b4 any possible myc grew. Do I just uncap the dish and transfer even though here is no myc. growth? just CWM?
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 15 hours, 37 minutes
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Re: Is there a good way to isolate agar cultures after cobweb mold has sporulated in it? [Re: DoSedLiving]
#19054387 - 10/29/13 09:28 PM (10 years, 3 months ago) |
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sorry man if the CW is all thats there you sound screwed
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DoSedLiving

Registered: 03/01/09
Posts: 632
Last seen: 5 years, 1 month
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Re: Is there a good way to isolate agar cultures after cobweb mold has sporulated in it? [Re: rumfor69]
#19054404 - 10/29/13 09:33 PM (10 years, 3 months ago) |
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I think there is some myc., its hard to tell though, ive never used agar, n this is strange shzz anyway.
I don't see why I could dunk in dilute bleach or 3% h2o2 before placing my sample on the agar? Couldn't you transfer to an new plate as soon as I see ANY growth?
BTW, I know CWM is the fuzzy crazy aggressive mold that invades your dish, that's obvious, but are there any other molds that look simialiar but aren't as aggressive?
Im wondering if the smaller, white, slower growths that are kinda, kinda fuzzy are a contam or my myc?
Edited by DoSedLiving (10/29/13 09:38 PM)
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