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Amazon Shop for: ½ Pint Jars, Brown Rice Flour, pH Test Strips

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OfflineFungi_x
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Why Just H2o?
    #1905379 - 09/11/03 02:48 PM (13 years, 2 months ago)

I'm doing a test with 1-pint of BRF (pf-tek) I did not use plain h2o, I placed 1-tablespoon of Light Corn Syrup into a measuring cup, Then I filled to 1/2 cup. and used that as the liquid just as if it were h2o.

My jars are colonizing very fast its been five days and my jars look about 14-17 days old.

Once I birth my jars (all my jars are tied up) I'm going to run some tests with substitutes to h2o or things to add to the h2o... I think I will try this kero water and v8 juice... I will have a few controlled jars with just normal pf-kek and compare results.

I kno a downside to the v8 juice may be harder to spot contams... but I have never had a contam so, ha.

Maybe I could get some more Ideas on from you guys.


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HAPPY FUCKING HOLIDAYS!


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OfflineBaby_Hitler
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Re: Why Just H2o? [Re: Fungi_x]
    #1905524 - 09/11/03 03:14 PM (13 years, 2 months ago)

Well, the V8 is probably acidic. You might need something to raise the pH.


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Invisibledeanofmean
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Re: Why Just H2o? [Re: Fungi_x]
    #1905887 - 09/11/03 04:13 PM (13 years, 2 months ago)

i like kero for liquid culture, only because it is cheaper and easer to find then dextrose .
but, for supplementing PF cakes, i think i'd go with somthing with less sugar . corn starch for example, or a small amount of powered milk . 
Edit: try beer  :nut:


Edited by deanofmean (09/11/03 04:20 PM)


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Offlineprocule
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Re: Why Just H2o? [Re: deanofmean]
    #1906447 - 09/11/03 06:15 PM (13 years, 2 months ago)

isn't dextrose a saccharide (sugar) ?


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home grown shit
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Invisibledeanofmean
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Registered: 12/07/02
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Re: Why Just H2o? [Re: procule]
    #1906719 - 09/11/03 07:51 PM (13 years, 2 months ago)

saccharide meaning, any of a series of compounds of carbon, hydrogen, and oxygen in which the atoms of the latter two elements are in the ratio of 2:1, especially those containing the group c6h10o5.
sucrose c12h22o11
maltose c12h22o11?h2O
glucose c6h12o6
dextrose c6h12o6?h2O
all of these work well in sterile culture, but must be kept sterile, because contams love it too .
any simple sugar works fine, but fungus doesn't seem to like refined cane sugar

Edit; i left out fructose c6h12o6 lol


Edited by deanofmean (09/11/03 08:27 PM)


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Invisibledaussaulit
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Re: Why Just H2o? [Re: Baby_Hitler]
    #1906943 - 09/11/03 08:55 PM (13 years, 2 months ago)

And v8 has a lot of sodium in it.

I don't think v8 is a good idea.


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