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Offlinesytar
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Jumping in to g2g
    #19049583 - 10/28/13 11:06 PM (10 years, 3 months ago)

I wanted to start a bulk grow with grains soon. I have an MS syringe. I was thinking the fastest way of getting the grow started would be to noc up a quarter pint or half pint jar with spores, let it colonize, then get my quart jars (8 of them) started with some g2g from the small jar. I was going to use WBS for the quart jars. After reading violet's tek I was thinking of using rgs for the quarter/half pint jar. Would it be stupid to use rgs to start a WBS jar? Will I even be saving myself any time by going the g2g route when I have no colonized grains already on hand?


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InvisibleDemonic_Chronic
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Re: Jumping in to g2g [Re: sytar]
    #19050071 - 10/29/13 01:43 AM (10 years, 3 months ago)

In my opinion if your thinking of starting a bulk grow the best route would be as follows,
Make 2 LC's (in case one contams) and once a decent network has been established (11-14) days just innoc your grain with your LC (2cc's per Qt Jar).
IDK if you mean rye grass seed when you say rgs but I dont know how well it will work with cubes, Id go for Rye berries or WBS.

:2cents:

DC


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OfflineSpacy
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Re: Jumping in to g2g [Re: Demonic_Chronic]
    #19050269 - 10/29/13 03:35 AM (10 years, 3 months ago)

I did g2g with multispore.
Normaly I use 1.5cc per jar, Now I used 3cc in the g2g jar.
So I get more genes in the jar.  I shaked the jar every 2 days
To mix the strains.
Then I inoculated 20 jars of the same size with the ms jar.
Had a nice yield from it.


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OfflinePussyFart
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Re: Jumping in to g2g [Re: Spacy]
    #19050288 - 10/29/13 03:50 AM (10 years, 3 months ago)

Quote:

Spacy said:
I did g2g with multispore.
Normaly I use 1.5cc per jar, Now I used 3cc in the g2g jar.
So I get more genes in the jar.  I shaked the jar every 2 days
To mix the strains.



Shaking the jar every 2 days is silly and pointless.

Shake them at 20-30% once and that's it.

Also you were not mixing the strains as they are already mixed.

And less is more with spores, it is not always a good thing to have more genetics.


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OfflineMidnight Cyclone
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Re: Jumping in to g2g [Re: sytar]
    #19050314 - 10/29/13 03:59 AM (10 years, 3 months ago)

Quote:

sytar said:
I wanted to start a bulk grow with grains soon. I have an MS syringe. I was thinking the fastest way of getting the grow started would be to noc up a quarter pint or half pint jar with spores, let it colonize, then get my quart jars (8 of them) started with some g2g from the small jar. I was going to use WBS for the quart jars. After reading violet's tek I was thinking of using rgs for the quarter/half pint jar. Would it be stupid to use rgs to start a WBS jar? Will I even be saving myself any time by going the g2g route when I have no colonized grains already on hand?




1. If you have MS, go MS. You're not really saving yourself any time by going other routes, including LCs which can be a pain to get the hang of if you're new. Don't use your time, effort or supplies on trying to make an LC at the moment.

2. Use WBS all the way. I've never heard of mixing grains but the hesitant feeling I get when I think about doing it personally makes me want to steer you clear of it.

3. G2G is only used to save time if you have the colonized grains already on hand.
The benefits that you get from G2G are,
            -Don't have to use all the spores you currently have.
            -You can get a test jar, to check the sterility of the syringe.


MS-G2G = 8 colonized quarts in about 18 days my time.

MS-LC = 11-14 days for LC, then another 10 days my time for fully colonized quarts. 21-24 days total my time. Assuming your LC hasn't fucked everything up.

I'm not into inoculating smaller jars and transferring to larger jars. It's a big chance for contamination, as well as pretty much a waste of time. See, when you inoculate a small container and let it colonize just to transfer  via G2G... you're essentially just inoculating a large jar, then wait for that small jar colonization time, and shake the big jar. Significantly less chance for contamination, and the same outcome. :thumbup:


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OfflinePussyFart
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Re: Jumping in to g2g [Re: Midnight Cyclone]
    #19050323 - 10/29/13 04:05 AM (10 years, 3 months ago)

Quote:

sytar said:
Would it be stupid to use rgs to start a WBS jar?



Not at all...it will work just fine.

Quote:

Midnight Cyclone said:
I'm not into inoculating smaller jars and transferring to larger jars. It's a big chance for contamination, as well as pretty much a waste of time.



How exactly does the size of the master jar effect the chance of contamming?

Quote:

Midnight Cyclone said:
See, when you inoculate a small container and let it colonize just to transfer  via G2G... you're essentially just inoculating a large jar, then wait for that small jar colonization time, and shake the big jar. Significantly less chance for contamination, and the same outcome. :thumbup:



Again, how does this increase the chance of contams?


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Offlinesytar
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Re: Jumping in to g2g [Re: PussyFart]
    #19050468 - 10/29/13 05:24 AM (10 years, 3 months ago)

NotaHacker: do you agree with the above posters that I wouldn't be saving any time making a small master jar then doing g2g? Seems like a lot of info in this thread is sketchy but I trust you.


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InvisibleSpitballJedi
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Re: Jumping in to g2g [Re: sytar]
    #19050544 - 10/29/13 06:00 AM (10 years, 3 months ago)

Hacker has the best advice so far.

Skip the LC.

Starting with a half pint jar of RGS is fine. I've done it many times with Rye Berries. It colonizes faster and gives plenty of inoculation points for 7 more Rye Berry jars.

These days, I just go ahead and make a 3/4 full quart though. The extra grains help the receiving jars colonize faster. But your method does work.


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Offlinesytar
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Re: Jumping in to g2g [Re: SpitballJedi]
    #19050597 - 10/29/13 06:17 AM (10 years, 3 months ago)

Yeah I'm not touching LC with a ten foot pole. I'll be starting agar soon but I want to get the grow underway ASAP. From what I understand making LC wouldn't speed things up at all unless you already had it on hand. If you do things right (test the LC on a PF jar ) LC just takes forever.


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Offlineblueconfusion
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Re: Jumping in to g2g [Re: sytar]
    #19050635 - 10/29/13 06:31 AM (10 years, 3 months ago)

What I do is noc up 7 quarts with my MS right of the bat wait for those to colonize then pick one use that for g2g and spawn the leftovers or save as more masters


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OfflineStromriderM
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Re: Jumping in to g2g [Re: blueconfusion]
    #19050660 - 10/29/13 06:38 AM (10 years, 3 months ago)

I tried using pint jars for grain masters a while back and I found that they colonized at about thesame rate as quart jars :shrug:


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OfflineJMcDoogle
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Re: Jumping in to g2g [Re: Stromrider]
    #19050693 - 10/29/13 06:50 AM (10 years, 3 months ago)

If it were me, how I am doing it - I knocked up 2-3 of each MS in RGS using pp5 half-pint containers, those will be for G2G Transfers to WBS which will be used to spawn my bulk - 1 of each of them will be fruited for enjoyment and spores.

I think violet has a point with RGS being a benefical, easy method of grain 2 grain.

I started four LC's the day my PC came and 2 days before I inoculated my first 7 quarts. They look great, but who really knows? I will have to grow them out in the long run to see if they've been contaminated or not.

---
Damnit, I have my lights off, and all this talk of inncoulation I just want to go up there and stare at them.

Although, its currently 65 down here, and im super comfortable wearing a sweatshirt, and 82 up there I get warm quickly.

Quick hijak - Should I keep the humidity levels up while the master grain jars are colonizing as to make sure the WBS jars with large breathing holes dont dry out?


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InvisibleHypnotoadCroaked
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Re: Jumping in to g2g [Re: Stromrider]
    #19052831 - 10/29/13 04:41 PM (10 years, 3 months ago)

Quote:

Stromrider said:
I tried using pint jars for grain masters a while back and I found that they colonized at about thesame rate as quart jars :shrug:



I have been using pint and half widemouth jars with milo for "master" jars(not 100% sure, but its very small kernels).  I would not go smaller than that for a G2g.  That amount of grain could easily be transferred to 8+ jars.  It is terribly convenient that I can get 8 into my pc.

I had pondered using pint jars for experiments, but the difference in time and effort to make a full quart is meaningless.  It just makes sense to use quarts sized jars.

I tried g2g a few months ago and have loved it.  My contamination rate fell from the standard 1 jar out of 7 to nothing at all.  I have not had one single contamination in a jar since I switched from the syringe to the still air box.  I have not touched a needle in over 3 months and have not looked back.

Oh and, steer clear of liquid cultures.  The benefit of accelerating the growth in the master jar is outweighed by the high risk of contamination.  G2g transfers are amazing fast colonizers.

Good luck!


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InvisibleSpitballJedi
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Re: Jumping in to g2g [Re: HypnotoadCroaked]
    #19052875 - 10/29/13 04:49 PM (10 years, 3 months ago)

Quote:

Whippy said:
I tried g2g a few months ago and have loved it.  My contamination rate fell from the standard 1 jar out of 7 to nothing at all.  I have not had one single contamination in a jar since I switched from the syringe to the still air box.  I have not touched a needle in over 3 months and have not looked back.

Oh and, steer clear of liquid cultures.  The benefit of accelerating the growth in the master jar is outweighed by the high risk of contamination.  G2g transfers are amazing fast colonizers.



:solidnod:


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OfflineMidnight Cyclone
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Re: Jumping in to g2g [Re: PussyFart]
    #19053225 - 10/29/13 05:48 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Quote:

sytar said:
Would it be stupid to use rgs to start a WBS jar?



Not at all...it will work just fine.

Quote:

Midnight Cyclone said:
I'm not into inoculating smaller jars and transferring to larger jars. It's a big chance for contamination, as well as pretty much a waste of time.



How exactly does the size of the master jar effect the chance of contamming?

You misunderstand me, that's not what I'm saying. I'm saying to just use larger jars from the start and avoid the G2G from one really small jar (OP said 1/4 pint.. which, if people use them, are only used for agar - from what I've seen) to a bunch of larger jars to "save time."

Quote:

Midnight Cyclone said:
See, when you inoculate a small container and let it colonize just to transfer  via G2G... you're essentially just inoculating a large jar, then wait for that small jar colonization time, and shake the big jar. Significantly less chance for contamination, and the same outcome. :thumbup:



Again, how does this increase the chance of contams?

You're inoculating a really small jar thinking it will colonize really fast -- WELL OF COURSE IT WILL, IT'S REALLY SMALL -- and trying to G2G to save time. You're adding the G2G vector for possible contamination. My proposition, as prior stated, is to just inoculate a couple quart jars of WBS and then shake them when time comes appropriate.




I set my answers in the above quoted text in bold underlining.


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OfflinePussyFart
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Re: Jumping in to g2g [Re: Midnight Cyclone]
    #19053249 - 10/29/13 05:54 PM (10 years, 3 months ago)

G2G is not a vector for contams if you have a good sterile technique.


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Offlineblueconfusion
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Re: Jumping in to g2g [Re: Midnight Cyclone]
    #19053282 - 10/29/13 06:00 PM (10 years, 3 months ago)

why wouldn't you just start with the larger quart jars, really you'll have more spawn to put into the quart jars you're g2g'ing and that will lead to faster colonization of the quarts in the long run.  and why not just go ahead and make several inoculations of the quart jars like 7 or 8 jars there will definitely be a jar that out preforms the others in colonization times which is ultimately what you're looking for right?


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OfflineStromriderM
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Re: Jumping in to g2g [Re: blueconfusion]
    #19053460 - 10/29/13 06:36 PM (10 years, 3 months ago)

I agree with blue. It's never a good idea to make only one grain master. You should make at least 3 or 4 in case there is an issue.

Also as I stated previously I inoculated a couple pint jars and a couple qt jars of wbs with an agar wedge a while back and they finished at almost the same time


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Offlinesytar
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Re: Jumping in to g2g [Re: Stromrider]
    #19053587 - 10/29/13 06:55 PM (10 years, 3 months ago)

I was actually thinking of making many extra-tiny grain masters and using the best, fastest growing ones to do my g2gs. But I'm also toying with the idea of just nocing with the ms syringe. trying to make this harvest happen in five weeks, a friend is moving out of country and I need him around when the harvest comes.

I'm not too concerned with the long run like blue seems to be. In the medium term I'll be using agar. I am most concerned with making this grow happen as fast as is reasonable.


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Edited by sytar (10/29/13 07:04 PM)


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Offlinesytar
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Re: Jumping in to g2g [Re: sytar]
    #19053658 - 10/29/13 07:08 PM (10 years, 3 months ago)


G2G is not a vector for contams if you have a good sterile technique.
-----
I'm not concerned about contams from g2g. My sterile technique is practiced. I went very heavy on lab classes for my biology degree.


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Edited by sytar (10/29/13 07:09 PM)


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