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OfflineDarkMadrigal
The wild
Registered: 08/06/12
Posts: 82
Last seen: 10 years, 22 days
Did I just kill my cubensis?
    #19048925 - 10/28/13 09:14 PM (10 years, 3 months ago)

I put my BRF jars of Cubensis in the freezer (instead of the fridge) to cold shock them. They were in there for about 15 hours. Are they toast?


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OfflineOgreLokon
Pretty Fun Guy
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Registered: 06/30/13
Posts: 512
Last seen: 6 years, 5 months
Re: Did I just kill my cubensis? [Re: DarkMadrigal]
    #19048967 - 10/28/13 09:22 PM (10 years, 3 months ago)

Yes. Cubes are a tropical species, and don't require a cold shock anyway.


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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
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Re: Did I just kill my cubensis? [Re: DarkMadrigal]
    #19048969 - 10/28/13 09:22 PM (10 years, 3 months ago)

y in the world would you do that? 
i have no idea if they are toast.  but id imagine so.  there is no need to cold shock cubensis.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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OfflineDarkMadrigal
The wild
Registered: 08/06/12
Posts: 82
Last seen: 10 years, 22 days
Re: Did I just kill my cubensis? [Re: DeadPhan]
    #19048993 - 10/28/13 09:25 PM (10 years, 3 months ago)

Hmm. I misread a tutorial that said to cold shock them in the fridge. They seem to look as they did now that they're adjusting. I guess I'll have to wait it out.


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
Trusted Cultivator
Re: Did I just kill my cubensis? [Re: DarkMadrigal]
    #19049000 - 10/28/13 09:26 PM (10 years, 3 months ago)

Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.


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OfflineRewindicus
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Registered: 06/05/11
Posts: 5,491
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Re: Did I just kill my cubensis? [Re: DarkMadrigal]
    #19049014 - 10/28/13 09:28 PM (10 years, 3 months ago)

Hmmmmmm. Good question. Myc is made of a lot of water and very thin cell walls. You just crystallized and expanded  it all very likely damaging/destroying the pathways. My vote is yes you jacked them. Likely killed it all even.

Sorry bud!

If it were me id still try to fruit them just to see for myself. But I think its a lost cause.


--------------------
“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.”- Dr. Seuss

"Too much of a good thing, can be wonderful!" - Mae West

"If you have nothing nice to say about anyone, come sit next to me."
- Alice Roosevelt Longworth





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OfflineDarkMadrigal
The wild
Registered: 08/06/12
Posts: 82
Last seen: 10 years, 22 days
Re: Did I just kill my cubensis? [Re: PussyFart]
    #19049042 - 10/28/13 09:33 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.

Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.

Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.

P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.

Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.

You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.

You want direct/intense 6500K light on a 12/12 schedule for fruiting.

Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.

Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.

The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.

Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.




That's all wonderful information, which annoys me more, as I'm told a hundred different things to take into account with 'growing'.

I generally kept my jars at 70 for a while, which colonized somewhat, but then read that they need to be at 80-82 for colonizing, so I ended up getting a heater to change the temperature of my closet. This didn't really change much of the reality. Now, here at one and a half months on my second try at this, I just feel like giving up.

I was just about to fruit them, and now it seems that I've screwed this whole project up through a stupid stoned blunder.

Right now I feel like giving up on this hobby. I want to love it, but, unlike growing plants, everything seems to be going wrong for me.


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Offlinesilverstem
Caps & Stems
Male


Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 6 years, 5 months
Re: Did I just kill my cubensis? [Re: Rewindicus]
    #19049227 - 10/28/13 10:04 PM (10 years, 3 months ago)

idk if they are dead.... maybe they will survive
Quote:

RogerRabbit said:
Last week, I brought some cultivation stuff in from storage in my open carport.  There were several petri dishes that had been colonized since before I moved here last summer.  After getting up to over 100F during the summer, and several weeks of -30F this winter, I was surprised to see all of them take off again once I brought them back in.

However, you're not going to colonize mycobags in that temperature.  Wait until you can move your stuff indoors, or wait until spring.
RR




Roger rabbit posted that 4 years ago...
Goodluck to you.


--------------------


Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????


Edited by silverstem (10/28/13 10:06 PM)


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OfflineRewindicus
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Registered: 06/05/11
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Re: Did I just kill my cubensis? [Re: silverstem]
    #19049544 - 10/28/13 10:59 PM (10 years, 3 months ago)

"Seems ive screwed this whole project up Through a stupid stoned blunder"


Heres some advice,

Until you get your techniques down and find a method that works for you.....dont do it inebriated. This hobby is pretty easy, forgiving even but there are some steps that need to be followed EXACTLY.

Also, if your following a tek that says to cold shock it sounds a bit dated. Follow the lets grow mushrooms videos exactly and you should have a successful grow. I had quite a few botched grows when I first started because I was lazy.


--------------------
“Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.”- Dr. Seuss

"Too much of a good thing, can be wonderful!" - Mae West

"If you have nothing nice to say about anyone, come sit next to me."
- Alice Roosevelt Longworth





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OfflineSynKyd
ctrl-alt-delite
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Registered: 09/27/13
Posts: 1,554
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Re: Did I just kill my cubensis? [Re: DarkMadrigal]
    #19049559 - 10/28/13 11:02 PM (10 years, 3 months ago)

Quote:

DarkMadrigal said:
I put my BRF jars of Cubensis in the freezer (instead of the fridge) to cold shock them. They were in there for about 15 hours. Are they toast?



  you probably killed them but it's a great story if you didn't-

:goat:

give em some time they may recover.


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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: Did I just kill my cubensis? [Re: SynKyd]
    #19049605 - 10/28/13 11:10 PM (10 years, 3 months ago)

you might be able to catch a buzz if you eat the cake.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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InvisibleMoorning Due
been know to derp


Registered: 10/08/13
Posts: 8,061
Loc: ether jet of existence
Re: Did I just kill my cubensis? [Re: DeadPhan]
    #19049869 - 10/29/13 12:24 AM (10 years, 3 months ago)

Don't trip bro. You will more than likely be OK. Keep a positive attitude and learn from your mistake.

Let us know, oK? but don't fuck it up more cuz your pissed.


--------------------
Cause we all have wings. But some of us don't know why...


My very own funnies thread: LINKY


Edited by Moorning Due (10/29/13 01:24 AM)


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OfflineDeadPhan
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Registered: 05/05/04
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Re: Did I just kill my cubensis? [Re: Moorning Due]
    #19049885 - 10/29/13 12:27 AM (10 years, 3 months ago)

im pretty sure mycelium is predominately composed of water.  as are almost, if not,  all life forms.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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InvisibleMoorning Due
been know to derp


Registered: 10/08/13
Posts: 8,061
Loc: ether jet of existence
Re: Did I just kill my cubensis? [Re: DeadPhan]
    #19049944 - 10/29/13 12:48 AM (10 years, 3 months ago)

Quote:

DeadPhan said:
im pretty sure mycelium is predominately composed of water.  as are almost, if not,  all life forms.




Trying to keep the mood positive man.. the guy is bumming...


--------------------
Cause we all have wings. But some of us don't know why...


My very own funnies thread: LINKY


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OfflineDeadPhan
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Registered: 05/05/04
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Re: Did I just kill my cubensis? [Re: Moorning Due]
    #19049946 - 10/29/13 12:49 AM (10 years, 3 months ago)

thats all good and grand.  but with false factual information?  :confused:


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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InvisibleMoorning Due
been know to derp


Registered: 10/08/13
Posts: 8,061
Loc: ether jet of existence
Re: Did I just kill my cubensis? [Re: DeadPhan]
    #19050033 - 10/29/13 01:22 AM (10 years, 3 months ago)

Your right, BUT... I think that some mycelium isn't just water, but also has some sugar carbohydrates floating around in it, which don't freeze the same way as pure water (think of a Popsicle), which would be how some fungi survive thru frosts in AL and Canada, etc.

Anyhow, I think it's species dependent, but go ahead and try dude! Don't get let down.


--------------------
Cause we all have wings. But some of us don't know why...


My very own funnies thread: LINKY


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