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urthtown
meat popsicle


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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contact sanitizers
#19046865 - 10/28/13 04:11 PM (10 years, 3 months ago) |
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Hello all,
I've been lurking here for a bit and one question I've had that I haven't been able to answer with the search function is:
Does anybody use a no-rinse, contact sanitizer to sterilize implements and equipment (SS bowls, thermometers, SGFC, plastic mono tubs, etc.)?
I use it all the time in my home brewing, sanitizing kegs and carboys. I know the two cultures are not the same, however, I am essentially creating a huge mono-culture of yeast in a malt syrup substrate and I can tell you sanitation is essential as there is a lot of free simple sugars up for grabs that a billion little beasties want to get their hands on.
I just started and I have been sanitizing bowls and things with my star-san or aseptox somewhat compulsively. I have 6 healthy cakes, two healthy hpoo straw tubs and 8 more hpoo straw pucks colonizing and no contams. (knock on wood)
Star-san (don't fear the foam)
For inoculation I have an alcohol lamp and I wipe my work surface with alcohol, make sure everything has been cleaned throughly but I just can't stop myself from sanitizing everything in sight with contact sanitizer anyway. Am I crazy? Is this stuff compatible with yeast based cultures but not with mycelium? It seems like I should be at least as careful with my mushroom cultures as I am with my yeasties - especially given the amount of fermentation (sauerkraut, veggies, kimchi, beer, cider, wine, mead.....) happening in the house....
Thanks, a humble student.
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 13 hours, 45 minutes
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Re: contact sanitizers [Re: urthtown]
#19047036 - 10/28/13 04:39 PM (10 years, 3 months ago) |
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It's not necessary. Soap and water can easily remove the vast majority of contaminants from plastic materials which is good enough for things that don't need to completely sterile like FCs, containers used during substrate preparation, etc.
The things that do need to be sterile need to be flame sterilized or pressure cooked to achieve sterility, after which trying to sanitize them could only risk recontaminating them.
For cleaning the area in general bleach works well. Alcohol is mainly useful for wiping down jars and lids just before inoculation and also to disinfect gloves after you get them on since it'd be inconvenient to do those thing with anything else since alcohol evaporates nice and quickly.
I'm assuming the contact sanitizer you're referring to is alcohol based.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: contact sanitizers [Re: urthtown]
#19047057 - 10/28/13 04:42 PM (10 years, 3 months ago) |
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Quote:
urthtown said: Does anybody use a no-rinse, contact sanitizer to sterilize implements and equipment (SS bowls, thermometers, SGFC, plastic mono tubs, etc.)?
No, because chemicals do not sterilize, they sanitize.
Most people sanitize everything that is not a tool with 70% alcohol.
If it must be sterile, then heat is needed.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: contact sanitizers [Re: PussyFart]
#19047324 - 10/28/13 05:20 PM (10 years, 3 months ago) |
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Star-san is only good for brewing. It's just to keep things clean enough that the massive pitch of yeast can take hold before anything else. See if star-san helps you out after brewing a sour beer, it wont, you'd have to autoclave all of your equipment to brew a non-sour again.
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urthtown
meat popsicle


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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Quote:
bodhisatta said: Star-san is only good for brewing. It's just to keep things clean enough that the massive pitch of yeast can take hold before anything else. See if star-san helps you out after brewing a sour beer, it wont, you'd have to autoclave all of your equipment to brew a non-sour again.
Yeah I have only dedicated sour equipment for this reason, but I couldn't help myself from rinsing out my SS bowl with sanitizer before mixing my pasteurized hpoo/straw with spawn! This all makes good sense - I knew it wasn't sterilizing but you are right about the massive pitch of yeast. A few spores ain't got nothing on a few hundred billion healthy yeasties getting pitched into their perfect habitat.
Thanks!
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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