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InvisiblePastywhyteMDiscord
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Re: Shaking wbs [Re: JMcDoogle]
    #19058967 - 10/30/13 05:49 PM (10 years, 2 months ago)

Quote:

JMcDoogle said:





A nice idea to do a side by side but. . . its meaningless if its not a monoculture :shrug: After my side pin and slatting experiments are concluded I plan to do something like this to see if a) it colonizes any quicker and b) to see if there are any differences in yield. I got just the isolate in mind as well.


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Re: Shaking wbs [Re: Pastywhyte]
    #19058996 - 10/30/13 05:55 PM (10 years, 2 months ago)

in my experience i've seen much slower colonization when i've shaken my jars before 20-30%.


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InvisiblePastywhyteMDiscord
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Re: Shaking wbs [Re: PirateSwazey]
    #19059412 - 10/30/13 07:21 PM (10 years, 2 months ago)

Quote:

PirateSwazey said:
in my experience i've seen much slower colonization when i've shaken my jars before 20-30%.




Oh I agree, I just want to do the experiment and post the results so I can have a ready link for people who don't seem to buy in to the idea. If my hypothesis is disproved by said experiment then fantastic, I may change my methods, or at least run another experiment with different genetics until consistent results are obtained.


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OfflineRubestoad
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Re: Shaking wbs [Re: Pastywhyte]
    #19059452 - 10/30/13 07:25 PM (10 years, 2 months ago)

IME ( one shook jar lol) the shaking revealed a contam the very next day. Is it possible then that a shake on a suspect jar might give you the verdict faster?


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InvisiblePastywhyteMDiscord
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Re: Shaking wbs [Re: Rubestoad]
    #19059484 - 10/30/13 07:29 PM (10 years, 2 months ago)

Quote:

Rubestoad said:
IME ( one shook jar lol) the shaking revealed a contam the very next day. Is it possible then that a shake on a suspect jar might give you the verdict faster?




I have done this and its not a bad way to reveal a contam but, that is the only time/reason that I would do that. Usually when I shake jars (especially more than once) I find that the colonization speed will slow incremetaly after each shake. Then when/if you get to the spawn run it takes forever :shrug:


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InvisibleMUSH HEAD420
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Re: Shaking wbs [Re: Pastywhyte]
    #19059513 - 10/30/13 07:34 PM (10 years, 2 months ago)

I am not doubting you have experience in the matter, but I do doubt that you shook a perfectly good jar more than once. More likely is that these jars you have shaken more than once were already problem jars.


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InvisiblePastywhyteMDiscord
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Re: Shaking wbs [Re: MUSH HEAD420]
    #19059694 - 10/30/13 08:00 PM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
I am not doubting you have experience in the matter, but I do doubt that you shook a perfectly good jar more than once. More likely is that these jars you have shaken more than once were already problem jars.




I have been impatient before. I will try most things once, ill advised or not. I do things that are recommended not to do all the time, just to see how they turn out. I have run no hole monos, no holes in bottom of SGFC, jar lids with no holes, not lined tubs and trays, used micropore tape instead of poly on monotub holes, used LC, tried hay as a sub, bucket tek, slatting tek, thin subs, thick subs, pint size pf cakes, popcorn, made FC's with foggers fans & pumps, ghetto ass agar, open air inoculations, outdoor inoculations, fractionalization, shaken jars after ms inoculation, etc, etc. I could probably fill a book with ill advised shit that I have tried.

Some of these things worked well for me, some were complete fails, some were just ok. Along the way I tried a lot of recommended shit that worked really well too. The result was I got a pretty good idea of what corners I can cut, which ones I can't, and why some shit that just seems easy doesn't work.

I have shaken grain master jars more than once :shrug:


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InvisibleMUSH HEAD420
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Re: Shaking wbs [Re: Pastywhyte]
    #19059783 - 10/30/13 08:11 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:


I have shaken grain master jars more than once :shrug:




Still though, shaking may or may not be what causes you to have a stalled myc.


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InvisiblePastywhyteMDiscord
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Re: Shaking wbs [Re: MUSH HEAD420]
    #19059910 - 10/30/13 08:31 PM (10 years, 2 months ago)

Quote:

MUSH HEAD420 said:
Still though, shaking may or may not be what causes you to have a stalled myc.




Ok I won't argue that. I think that I am now resolved to do a side by side experiment, just to get to the bottom of this, I have seen a few people questioning this, and I know of at least one TC that swore by multiple shakes. If and when I complete this side by side results shall be posted.


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InvisibleMUSH HEAD420
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Re: Shaking wbs [Re: Pastywhyte]
    #19059938 - 10/30/13 08:34 PM (10 years, 2 months ago)

:drag:


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OfflineJMcDoogle
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Re: Shaking wbs [Re: MUSH HEAD420]
    #19063649 - 10/31/13 02:02 PM (10 years, 2 months ago)

An update before work, no pictures as of right now, battery is charging -


The one I shook is doing remarkably well, perhaps 30-50% colonized ( depending on center that cannot be seen )

Compared to 10-15% of the unshaken -

All in all, we will see in the long run.


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Re: Shaking wbs [Re: Pastywhyte]
    #19063847 - 10/31/13 02:50 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

MUSH HEAD420 said:
Still though, shaking may or may not be what causes you to have a stalled myc.




Ok I won't argue that. I think that I am now resolved to do a side by side experiment, just to get to the bottom of this, I have seen a few people questioning this, and I know of at least one TC that swore by multiple shakes. If and when I complete this side by side results shall be posted.



Like citric I am a multiple shake kinda guy. Done it with iso's and ms alike and any stalling that happened was due to a contam and have never seen a healthy jar stall due to shaking, if that were the case g2g would be a littleriskier


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Offlineblueconfusion
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Re: Shaking wbs [Re: cronicr]
    #19063928 - 10/31/13 03:06 PM (10 years, 2 months ago)

IME a jar shaken at 30% will more evenly distribute the grains from 30% my hats usually colonizers in about 4 or less days


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Re: Shaking wbs [Re: blueconfusion]
    #19065551 - 10/31/13 07:40 PM (10 years, 2 months ago)

The shaken one is going so quick I think its off to the fridge so the other ones can catch up to it.


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Re: Shaking wbs [Re: MUSH HEAD420]
    #19065775 - 10/31/13 08:17 PM (10 years, 2 months ago)

No need for the fridge, I just fruited a fout month old grain jar with np's and the fruits pack quite a punch


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Re: Shaking wbs [Re: cronicr]
    #19065875 - 10/31/13 08:33 PM (10 years, 2 months ago)

thanks, no fridge.


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Re: Shaking wbs [Re: MUSH HEAD420]
    #19065951 - 10/31/13 08:41 PM (10 years, 2 months ago)

Fridges are dirty anyway lol


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Re: Shaking wbs [Re: cronicr]
    #19065966 - 10/31/13 08:43 PM (10 years, 2 months ago)

so no moldy bread in the fruiting chamber then?


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Re: Shaking wbs [Re: MUSH HEAD420]
    #19066019 - 10/31/13 08:50 PM (10 years, 2 months ago)

Unless you like yeast infections....


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Re: Shaking wbs [Re: cronicr]
    #19066416 - 10/31/13 09:48 PM (10 years, 2 months ago)

Hmm could possibly break up one of these giant white pills in med cabinet and add to sub to kill the yeast?


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