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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Not sure if my mycelium cake is colonized?
    #19042843 - 10/27/13 09:23 PM (10 years, 3 months ago)

I've got two jars that have been colonizing for over 3 weeks. Over the last week the myc growth seemed to stall, here is 2 different photo angles of the same jar. Are my jars ready?should I wait another week?what do you guys suggest? (I used brf and verm)






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Offlinerumfor69
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Re: Not sure if my mycelium cake is colonized? [Re: TheLoopIs]
    #19042854 - 10/27/13 09:25 PM (10 years, 3 months ago)

what are your holes like in the lids?


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Invisiblehuxley_hound
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Re: Not sure if my mycelium cake is colonized? [Re: rumfor69]
    #19042878 - 10/27/13 09:32 PM (10 years, 3 months ago)

Definitely not time imo

Did only two of the four inoculation points take?

Once the mycelium can do nothing further with the substrate, I think you should see in vitro pinning. but someone else should verify that


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Not sure if my mycelium cake is colonized? [Re: huxley_hound]
    #19042892 - 10/27/13 09:34 PM (10 years, 3 months ago)

my holes are just two plain holes on opposites sides


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Offlineoneloveoneloveonel
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Re: Not sure if my mycelium cake is colonized? [Re: TheLoopIs]
    #19043157 - 10/27/13 10:36 PM (10 years, 3 months ago)

I had a cake that never fully colonized.  After about three months I just took a spoon to the substrate spot and fruited it finally.  The cake did fine in my shot gun fruiting chamber (around a dozen pins) but the mycelium took hold of some the vermiculite I rolled it in because it wasn't finished colonizing. It looked really weird and scared me because I thought it was a contam!

I've researched that if the bottom of the jar is taking too long to colonize you should flip the jar upside down to release some CO2 or something like that.  Give it a try and if it's still not making any improvements you can just follow my lil tek :smile:

Good luck.


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InvisiblePastywhyteMDiscord
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Re: Not sure if my mycelium cake is colonized? [Re: oneloveoneloveonel]
    #19043168 - 10/27/13 10:39 PM (10 years, 3 months ago)

Give it time, its all you can do really :shrug: Next time use widemouth half pints and 4 inoculation points, should colonize better.


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OfflineJMcDoogle
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Re: Not sure if my mycelium cake is colonized? [Re: Pastywhyte]
    #19043180 - 10/27/13 10:42 PM (10 years, 3 months ago)

Another fair assumption is besides the co2 stall would be too high of a mositure content on the bottom half of the growing medium.

Gravity brings the water to the bottom, creating excessive moisture.

At the very least you could still use the cake, but make sure to rid of all of the uncolonized growing medium as to avoid contamination spreading across to the mycellium.


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Offlinerumfor69
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Re: Not sure if my mycelium cake is colonized? [Re: TheLoopIs]
    #19044563 - 10/28/13 07:10 AM (10 years, 3 months ago)

Quote:

TheLoopIs said:
my holes are just two plain holes on opposites sides




two will work a lot of people use 4 but it's ok. Stalling can be from

contamination, too tightly packed jars, moisture being wrong. You'll just

have to wait and see if it covers the entire jar with mycelium then wait

another week after that for them to consolidate before birthing them.


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InvisibleFungi
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Registered: 09/29/13
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Re: Not sure if my mycelium cake is colonized? [Re: rumfor69]
    #19044655 - 10/28/13 08:11 AM (10 years, 3 months ago)

I have a similar issue, it seems that the patch where MYC didnt grow on is a little off white, like as if there is some slight white growth on it but not quiet the regular MYC...

Is it a good idea just to leave it for a few more weeks until the jars start pinning themselves, as that is a good indicator to take them out and pop into fruiting chamber?


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Not sure if my mycelium cake is colonized? [Re: Fungi]
    #19046143 - 10/28/13 01:38 PM (10 years, 3 months ago)

thanks for the awesome tips guys! ill turn my jars upside down. So i can literally take a knife to my cake and cut off the uncolonized part?:O


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OfflineOgreLokon
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Re: Not sure if my mycelium cake is colonized? [Re: TheLoopIs]
    #19046179 - 10/28/13 01:48 PM (10 years, 3 months ago)

don't flip them- CO2 doesn't fall, and you run the risk for contams if you do

I had a cake that did that, and I pulled it out, and washed the uncolonized part out before dunking


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OfflineJMcDoogle
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Re: Not sure if my mycelium cake is colonized? [Re: OgreLokon]
    #19047572 - 10/28/13 05:53 PM (10 years, 3 months ago)

Be careful when handling not to bruise the mycellium, wash your hands with alcohol/perioxide and allow to dry, put on some gloves, wash them too with alcohol.

Do you have a still air box?

I will let others direct you on removing the contaminated via water or simply breaking off with fingers, im guessing cutting isnt the first option.


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Offlinerumfor69
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Re: Not sure if my mycelium cake is colonized? [Re: Fungi]
    #19052893 - 10/29/13 04:53 PM (10 years, 3 months ago)

Removing a contam is not really a good idea, sure it can work. But if it

doesn't and it shoots spores all over your grown room your going to have

worse and worse grows with more problems as you continue.

Quote:

Fungi said:
I have a similar issue, it seems that the patch where MYC didnt grow on is a little off white, like as if there is some slight white growth on it but not quiet the regular MYC...

Is it a good idea just to leave it for a few more weeks until the jars start pinning themselves, as that is a good indicator to take them out and pop into fruiting chamber?




Sometimes where the discolored and the myce meet you will see like

almost a wall or line between the two like they wont mix. That's def

a contam if so.


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Edited by rumfor69 (10/29/13 04:58 PM)


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InvisibleFungi
Psycho4ctive


Registered: 09/29/13
Posts: 393
Loc: Melbourne Flag
Re: Not sure if my mycelium cake is colonized? [Re: rumfor69]
    #19053043 - 10/29/13 05:20 PM (10 years, 3 months ago)

If its contaminated, should I discard the cake? What's the worst that can happen?


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To Fathom Hell or Soar Angelic, Just Take a Pinch of Psychedelic



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OfflinecronicrFacebook
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Re: Not sure if my mycelium cake is colonized? [Re: Fungi]
    #19053084 - 10/29/13 05:27 PM (10 years, 3 months ago)

Yes discard it.....wuts the best that can happenlol ..a few shrooms possibly popping up isn't worth the risk of more contams


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Offlinerumfor69
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Re: Not sure if my mycelium cake is colonized? [Re: rumfor69]
    #19053319 - 10/29/13 06:08 PM (10 years, 3 months ago)

Quote:

Fungi said:
If its contaminated, should I discard the cake? What's the worst that can happen?





Quote:

rumfor69 said:
But if it doesn't and it shoots spores all over your grow room your going to have

worse and worse grows with more problems as you continue.






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Links For Beginners And Beyond:mspoil:


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Invisiblehuxley_hound
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Re: Not sure if my mycelium cake is colonized? [Re: TheLoopIs]
    #19053983 - 10/29/13 08:00 PM (10 years, 3 months ago)

Quote:

TheLoopIs said:So i can literally take a knife to my cake and cut off the uncolonized part?:O




Yes. I had to do it recently. As long as you're careful, the cake will fruit, as my fragment is now doing.

You can use a knife, spoon, whatever...just make sure to get anything that is uncolonized and check the rest of the cake very carefully for irregularities suggesting contamination.


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InvisibleTheLoopIs
Registered: 05/29/13
Posts: 330
Re: Not sure if my mycelium cake is colonized? [Re: huxley_hound]
    #19062304 - 10/31/13 09:04 AM (10 years, 3 months ago)

I'lll use a knife. When it comes to using alcohol to clean my knife/hands... won't the mycelium suck that up and eventually I'll be ingesting that yucky alcohol?:/


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InvisibleFungi
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Registered: 09/29/13
Posts: 393
Loc: Melbourne Flag
Re: Not sure if my mycelium cake is colonized? [Re: TheLoopIs]
    #19062664 - 10/31/13 10:37 AM (10 years, 3 months ago)

After all this time my cakes look contam free. The grey looking spots have vanished. Did the cubensis myc kick some ass or what?


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OfflinecronicrFacebook
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Re: Not sure if my mycelium cake is colonized? [Re: Fungi]
    #19062693 - 10/31/13 10:44 AM (10 years, 3 months ago)

My guess is it was cube myc trying to colonize a compact sub


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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