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OfflineN2ocean
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What is causing this cobweb?
    #19038325 - 10/26/13 10:27 PM (10 years, 3 months ago)

Sorry for another cobweb post. But I'm also curious if my current growing strategy is sound.

I spawn 5 rye grain jars to 1brick coir/2quarts verm, by the 50-50 tek.  I cover the top w/ a thin layer of verm/coir just to cover all of the exposed rye berries.  Is this a good idea?  I feel like this might only be introducing a small unnecessary casing layer...

I spawn to Tupperware containers (pics) then snap the covers w holes on them.  With this first grow I filled the containers too high w/ sub and it might be evident in the pics but the top bit of sub got compressed and flattened against the top.  Will this cause any major problems? did this possibly cause the cobweb?

Other problems w/ this batch... I messed up w/ pasteurization and cooked the sub up to 220 degrees for a bit.  I cooled it and spawned anyway hoping for the best :/ most likely a mistake.

I sprayed these w/ 3% h2o2 and will repeat every 12 hrs a few times.  This isn't too far along to make that useless is it?

any other thoughts would be appreciated...  Thanks!!!!

Here's those pictures







--------------------
---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology  An excellent, rather short read here----


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Invisibleelasticaltiger
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Re: What is causing this cobweb? [Re: N2ocean]
    #19038352 - 10/26/13 10:31 PM (10 years, 3 months ago)

Are you sure that's cobweb dude?  Can you get a closer picture of it?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

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Offlinejackalope9517
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Re: What is causing this cobweb? [Re: elasticaltiger]
    #19038371 - 10/26/13 10:34 PM (10 years, 3 months ago)

That doesn't look like cobweb.


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Invisibleelasticaltiger
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Re: What is causing this cobweb? [Re: jackalope9517]
    #19038395 - 10/26/13 10:36 PM (10 years, 3 months ago)

Quote:

jackalope9517 said:
That doesn't look like cobweb.




My first reaction.  It looks like mycelium but I'm certainly no expert.  If it was cobweb wouldn't it be all over the top layer and not just localized around the mycelium?  I just can't see the dividing line between where mycelium ends and cobweb begins.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlinejackalope9517
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Re: What is causing this cobweb? [Re: elasticaltiger]
    #19038428 - 10/26/13 10:40 PM (10 years, 3 months ago)

Yea cobweb is really wispy and grey as in it sometimes looks like an old ladies hair. It also wouldn't be alongside the mycelium like that, it would be another lair completely covering the mycelium and the substrate. If it was infested with cobweb i believe we wouldn't even be able to see through the grayness of the top.


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OfflineN2ocean
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Re: What is causing this cobweb? [Re: elasticaltiger]
    #19038504 - 10/26/13 10:52 PM (10 years, 3 months ago)

i was pretty sure, i'll try and get in a bit steadier with the shot, hmmm.  well these next couple pictures might not be of much use but maybe they will.  i forgot that i already blasted this with h2o2, which is lovely considering you guys don't think it is...  I guess my  area of concern was what looks like it's growing into the white mycellium (bad description). on the zoomed out version there's two distinct shades. the really white mycelium, and the more greyish "whispier" stuff.  In the original closer picture it looks as if the darker, whispy growth is a part of the white mycelium, but to the eye it seemed very distinct and separate from the other growth... Here's the next pictures, if they help,  Thanks!





EDIT: some full res image links
Before h202
http://i.imgur.com/wVbAHil.jpg

http://i.imgur.com/fY15pPv.jpg
After h202
http://i.imgur.com/8t1HeEO.jpg

http://i.imgur.com/dlNPDD2.jpg


--------------------
---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology  An excellent, rather short read here----


Edited by N2ocean (10/26/13 10:55 PM)


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Invisibleelasticaltiger
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Re: What is causing this cobweb? [Re: N2ocean]
    #19038667 - 10/26/13 11:15 PM (10 years, 3 months ago)

Stop spraying with h2o2 right away.  Look at these pictures http://www.shroomery.org/forums/showflat.php/Number/17231313#17231313 of cobweb.  I really don't feel like that's the situation you have.  When you spawn to coir the mycelium can look cobwebby in between the stronger inoculation points.



That's a pretty standard looking colononized coir/verm substrate.  I thought my first monotub was covered in cobweb when I first saw it but it turns out that's just how they look sometimes.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflinePsyderman
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Re: What is causing this cobweb? [Re: N2ocean]
    #19038697 - 10/26/13 11:21 PM (10 years, 3 months ago)

I don't see any cobweb on there on either the before or after pics. Is that tub still colonizing and you took off the lid for pics or did you already introduce fruiting conditions? It looks like you tub is in an FC.
If it is still colonizing put the lid back on and give it a day or two and see if the "cobweb" continues to grow.
If it is in the FC, how long has it been in there?

You mention a coir/verm layer on top to cover the berries. Do you pasteurize the casing before throwing it on top or do you just hydrate and  spread?

How long was your sub at 220 during pasteurization?

Also remember when you are expanding your jars to bulk, make sure you leave a passive opening for GE during the colonization stage. Maybe drill a hole in the top of the Tupperware containers and stuff with poly-fil or cover with micro pore tape.


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Invisibleabltsandwich
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Re: What is causing this cobweb? [Re: Psyderman]
    #19038743 - 10/26/13 11:30 PM (10 years, 3 months ago)

220 for pasteurization? I know that ain't Kelvin or Celsius and certainly not Fahrenheit.


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OfflineMisterDeadeye


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Re: What is causing this cobweb? [Re: abltsandwich]
    #19038821 - 10/26/13 11:58 PM (10 years, 3 months ago)

What you need to do is cover those holes with some kind of filter(micropore tape will work) and keep it closed for a few days. That is not completely colonized. Can you show us side and bottom pictures? And how thick was the top layer? It was not a casing if you covered the top while you were spawning. It'll just potentially take longer because of unequal mycelium distribution. You don't have cobweb, you have mycelium that you sprayed with H2O2. And if you sprayed a lot, the colonization will likely take longer because of shock.

Also, that substrate is very thin. You've potentially failed this grow if you have had the lid off a lot; thin substrates can't hold moisture like thicker substrates can. You also didn't say how long it's been since you spawned. Regardless, you need to put it back into colonization mode for a while.

Lastly, pasteurization temperatures are important. Be careful with the next few stages of this grow. You don't want to let any mold sporulate because your clothes, body, and house will be carrying spores. Those aren't easy to get rid of.


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OfflineN2ocean
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Re: What is causing this cobweb? [Re: MisterDeadeye]
    #19040249 - 10/27/13 11:01 AM (10 years, 3 months ago)

phew, thanks for the advice.  It's been in the fruiting chamber for about 14 hrs. now, I'll put the lids back on (w/ micropore tape over the holes) and put it back in colonization mode after this post.  Would that be unwise at this point?  I did spray kinda heavily w/ h2o2. 

During pasteurization I forgot about the sub and let temps get too high.  It was above 190 for probably 1/2hr-45 min w/220 being max.

Edit: psy it wasn't a casing layer persay, it was just a thin layer of the same coco/verm sub sprinkled on top.

Edit 2:  Pics of the sides and bottom.









--------------------
---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology  An excellent, rather short read here----


Edited by N2ocean (10/27/13 11:38 AM)


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Offlinenn-IlliniSpiralDMT
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Re: What is causing this cobweb? [Re: N2ocean]
    #19040272 - 10/27/13 11:12 AM (10 years, 3 months ago)

That isn't cobweb you thread jacker!:mad2:

Just kidding, I am not mad:thumbup:


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OfflinePsyderman
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Re: What is causing this cobweb? [Re: nn-IlliniSpiralDMT]
    #19040440 - 10/27/13 11:56 AM (10 years, 3 months ago)

The sides and bottom look good. You are almost fully colonized. You just want that top to finish to reduce the chance of contams considering you essentially performed sterilization to your sub. Give it another day or two  and you should see those last few spots colonize, you want a nice white blanket to cover that sub. Then bust that baby open and throw it in your FC and wait for pinny goodness to appear. Good luck man, can't wait to see the finished product.

Also if you continue to use the Tupperware lids fewer holes would be better, you want just one or two for the GE. And that tape looks like medical tape not Micropore. Not sure if medical tape breathes all to well.


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Offlinejackalope9517
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Re: What is causing this cobweb? [Re: Psyderman]
    #19040733 - 10/27/13 01:12 PM (10 years, 3 months ago)

Ahh i know what you're talking about OP. Yea actually i was concerned with my mono because there were two different shades of mycelium and one was more grayish and whispy. Turns out, after being patient, practically overnight hyphal knots turned up all over the grayish mycelium and it's all completely white now and pinning nicely. Fruits aren't that great, because it's MS, but hey better than i hoped.


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OfflineN2ocean
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Re: What is causing this cobweb? [Re: jackalope9517]
    #19042803 - 10/27/13 09:11 PM (10 years, 3 months ago)

Quote:

jackalope9517 said:
Ahh i know what you're talking about OP. Yea actually i was concerned with my mono because there were two different shades of mycelium and one was more grayish and whispy. Turns out, after being patient, practically overnight hyphal knots turned up all over the grayish mycelium and it's all completely white now and pinning nicely. Fruits aren't that great, because it's MS, but hey better than i hoped.




yea, i should've known, or at least been a little more patient... Oh well, i'm optimistic about its recovery :bow2:  thanks for the help everyone :] i'll try and post pics in the future of success/failure


--------------------
---The mystics subjective experience of his identity with "the All" is the scientist's objective description of the ecological relationship of the organism/environment as a unified field - Alan Watts, The Joyous Cosmology  An excellent, rather short read here----


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Offlinejackalope9517
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Re: What is causing this cobweb? [Re: N2ocean]
    #19042945 - 10/27/13 09:48 PM (10 years, 3 months ago)

Quote:

N2ocean said:
Quote:

jackalope9517 said:
Ahh i know what you're talking about OP. Yea actually i was concerned with my mono because there were two different shades of mycelium and one was more grayish and whispy. Turns out, after being patient, practically overnight hyphal knots turned up all over the grayish mycelium and it's all completely white now and pinning nicely. Fruits aren't that great, because it's MS, but hey better than i hoped.




yea, i should've known, or at least been a little more patient... Oh well, i'm optimistic about its recovery :bow2:  thanks for the help everyone :] i'll try and post pics in the future of success/failure



No problem it'll be all well. What i suggest is, in the future, posting the pics as soon as you see a suspicious potential contam so that way you know quickly whether to keep or to toss. Everyone here would love to help out :smile:


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OfflineMisterDeadeye


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Re: What is causing this cobweb? [Re: jackalope9517]
    #19043206 - 10/27/13 10:48 PM (10 years, 3 months ago)

Quote:

jackalope9517 said:
Quote:

N2ocean said:
Quote:

jackalope9517 said:
Ahh i know what you're talking about OP. Yea actually i was concerned with my mono because there were two different shades of mycelium and one was more grayish and whispy. Turns out, after being patient, practically overnight hyphal knots turned up all over the grayish mycelium and it's all completely white now and pinning nicely. Fruits aren't that great, because it's MS, but hey better than i hoped.




yea, i should've known, or at least been a little more patient... Oh well, i'm optimistic about its recovery :bow2:  thanks for the help everyone :] i'll try and post pics in the future of success/failure



No problem it'll be all well. What i suggest is, in the future, posting the pics as soon as you see a suspicious potential contam so that way you know quickly whether to keep or to toss. Everyone here would love to help out :smile:




Truth. We've all been beginners at some point.


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