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OfflineAnao
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490 ml jars, doomed to fail?
    #19041949 - 10/27/13 06:16 PM (10 years, 3 months ago)

So, I hadn't done enough research when I started this grow and even though I know it's not advised to use BRF cakes as spawn, I just decided to do so, based on successful experiences from friends I know.

Then, while it was colonizing I just got paranoid and began doing some research, and I think my jars may be inappropriate for a variety of reasons. It has been 11 days since inoculation, I'm not sure if it's on time, it's not rhizo because I also put too much spores into the solution. So, as you can see not everything was done following the tek.
Also, while doing the substrate I got the ratio wrong. But I noticed it was way too moist and put more vermiculite on it, more or less making up for it. So water content should be fine.
The reason colonization may not be on time is because on the first week they were at 21-25ºC degrees range. Since then, 28-30ºC.

There are also some experimental jars with popcorn which are taking much longer, maybe because I didn't simmer them they're probably too tough. Around 10%.

So I know my teks aren't flawless and they're probably not the best. Just asking in your opinion, do I have any chance?

Analyzing the jars format, will they stall or will I be able to spawn them? They're 490ml.

Thanks.



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Offlinerumfor69
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042178 - 10/27/13 07:05 PM (10 years, 3 months ago)

Ya know considering what ya did they dont look all that bad. I dont see any bad colors. Do they have filters on top or just small holes? Can you smell through the holes see if they smell bad or too sweet.


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Offlinerumfor69
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Re: 490 ml jars, doomed to fail? [Re: rumfor69]
    #19042184 - 10/27/13 07:06 PM (10 years, 3 months ago)

The one less white jar looks a little slimy at the bottom and might have bacteria in it but its' hard to tell


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OfflineAnao
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042218 - 10/27/13 07:11 PM (10 years, 3 months ago)

They're completely sealed atm. I don't tend to open holes until colonization has stalled. They took a while until any signs of mycelium (around 4-5 days, it has been exponentially faster) because of the low temperatures, one day it came as low as 18ºC. It has been 5 days since I put temps into the right range.

I can't see anything wrong about myceia growth or anything, but I've read smaller mouth jars, and too tall jars, tend to just stall because of some reasons. These are not 1/2 pints, which are the standard tek. This may be just a fake growth, which I hope is not. So I was wondering if anyone could tell that by the format.


Edited by Anao (10/27/13 07:18 PM)


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Offlinerumfor69
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042273 - 10/27/13 07:21 PM (10 years, 3 months ago)

No gas exchange(or holes) can lead to contams growing better like bacteria. Fungi has to breath just barely when colonizing. Even pin holes
would be better than nothing.

The one thats more white isn't "fake growth" that is myce for sure


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OfflineAnao
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Re: 490 ml jars, doomed to fail? [Re: rumfor69]
    #19042313 - 10/27/13 07:31 PM (10 years, 3 months ago)

I heard that it was not needed until later on the colonization. Can you confirm they're benefical at all phases? I can make them right now.
Also, when I make GE holes do I need to turn the jars upside down? I heard that if you doesn't do that it's useless.

I heard that 1 pint jars (these are 490ml, so slightly bigger than 1pint) would stall colonization at bottom because of too much pressure, especially on tall jars. So it doesn't seem my case?


Thanks for the answers.


Edited by Anao (10/27/13 07:33 PM)


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OfflinePussyFart
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042325 - 10/27/13 07:34 PM (10 years, 3 months ago)

Quote:

Anao said:
I heard that it was not needed until later on the colonization. Can you confirm they're benefical at all phases?



You want high co2 for colonization, but you need some kind of filtered GE or the mycellium will stall.

Quote:

Anao said:
Also, when I make GE holes do I need to turn the jars upside down? I heard that if you doesn't do that it's useless.



No.....nothing goes upside down.

Co2 will not fall out of the holes as it does not sink.

Co2 rises with the humidity and heat currents, so the GE holes should be on top.

Grammatical genius.......


Edited by PussyFart (10/27/13 07:34 PM)


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Offlinerumfor69
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Re: 490 ml jars, doomed to fail? [Re: PussyFart]
    #19042340 - 10/27/13 07:37 PM (10 years, 3 months ago)

:whathesaid:


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OfflineAnao
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042343 - 10/27/13 07:37 PM (10 years, 3 months ago)

Yeah, I guess after almost 2 weeks it would be fine to open some holes. So CO2 is needed for germination mostly?

I'll make some tiny holes and seal with micropore. Sorry for my grammar, I'm not american.

Thanks for the tips. If you could back it up with some technical information it would be awesome, though.


Edited by Anao (10/27/13 07:38 PM)


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Offlinerumfor69
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042353 - 10/27/13 07:40 PM (10 years, 3 months ago)

Fungi breathes oxygen at all stages of life. A little gas exchange trickle needs to be there at all stages in the jar or the myce will stop growing and die


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OfflineAnao
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Re: 490 ml jars, doomed to fail? [Re: rumfor69]
    #19042469 - 10/27/13 08:06 PM (10 years, 3 months ago)

Made lots of really tiny holes, with a sterilized syringe and covered with micropore. After making sure they were complete safe, I tried to smell and couldn't smell anything. I guess that's good news?


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OfflinePussyFart
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042490 - 10/27/13 08:10 PM (10 years, 3 months ago)

You did not have to cover the holes with tape.

Leaving the dry verm layer exposed will not hurt anything.

In fact it's actually a good thing  to keep them holes exposed.......it keeps the dry verm layer dry.

Remember, the dry verm layer is your filter...nothing else is needed.


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OfflineAnao
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Re: 490 ml jars, doomed to fail? [Re: PussyFart]
    #19042503 - 10/27/13 08:12 PM (10 years, 3 months ago)

Due to the lack of vermiculite (I didn't notice until I was making the recipe) I couldn't make a vermiculite layer on them. This is another thumbs down, but covering the holes is justified then?

I need to buy some vermiculite ASAP.


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Offlinerumfor69
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042508 - 10/27/13 08:13 PM (10 years, 3 months ago)

No news is good news i guess. Pin holes covered in miropore tape

prolly isn't going to be good enough. You should look into proper

rye berries or wild bird seed teks that use synthetic filter disks

and jars with lids with holes. Your kinda way off on all this sorry

to say :frown: no offense to you.


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OfflinePussyFart
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Re: 490 ml jars, doomed to fail? [Re: Anao]
    #19042512 - 10/27/13 08:14 PM (10 years, 3 months ago)

If there is no dry verm layer you have already failed.

Get some verm and start over.

Foil is not a filter.

Those jars are all most likely contaminated.


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OfflineAnao
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Re: 490 ml jars, doomed to fail? [Re: rumfor69]
    #19042525 - 10/27/13 08:16 PM (10 years, 3 months ago)

I thank your input and hope otherwise. Until contaminants really show up I'm keeping up with it, anyway.

Thanks.


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Invisiblehuxley_hound
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Re: 490 ml jars, doomed to fail? [Re: PussyFart]
    #19042579 - 10/27/13 08:30 PM (10 years, 3 months ago)

Well, if you get a nasty contamination then you might spoil your space for future grows. The lack of vermiculite moderating gas exchange from the room's atmosphere into the jar's micro-atmosphere "goes the other way", too, meaning that your lack of verm won't keep nasties in the jar from spreading to the air in your room.

So, you might wanna keep that in mind. I think it would perhaps behoove you to toss these and start afresh. I don't think it's worth continuing this when you look at the factors working against success. :thumbup:

And I have to laugh at the grammar comments in here. Sorry.:tongue:

Edit: Good luck to you, whatever you decide to do! Keep us posted.


Edited by huxley_hound (10/27/13 08:32 PM)


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OfflineAnao
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Re: 490 ml jars, doomed to fail? [Re: huxley_hound]
    #19044173 - 10/28/13 03:18 AM (10 years, 3 months ago)

Wow, thanks for the kind answer. Whenever I get contams, I PC them for ~1h and then dump. I guess its not the safest method, but worked so far.

I thought I would correct myself and say I did use the dry vermiculite layer. I still don't feel safe about leaving it so open. Looks unnecessarily risky IMHO, I'll keep it in mind in case they stall, though.


Edited by Anao (10/28/13 03:21 AM)


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