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Offlinejunkjunkie2006
Stranger

Registered: 09/22/13
Posts: 1
Last seen: 10 years, 3 months
Trouble with... well, everything.
    #19041073 - 10/27/13 02:23 PM (10 years, 3 months ago)

I know a guy who is trying to cultivate some mushrooms. He has grown PF before and had good luck and also done PF jars to bulk straw/poo sub and had fair to good results.

He is trying to do some grain to straw/poo but everything keeps going wrong. He has Quart jars that were filled with WBS at first and they colonized. very slowly and when I say slow I mean slow, like 3-4weeks. Also had a high failure rate (possibly due to long colonization time) He has been using multi-spore syringe (made from spore prints that were made in less than sterile conditions) also experimented with a LC made with the same multi-spore. He has had a few jars come out healthy but they were very SLOW. One of the healthy jars was used to do a micro grow with straight WBS and got healthy but small fruits.

Also attempted using RGS and had slightly faster colonization but still slow. RGS seems to be too wet after PC-ing usually has goey brown ick in the bottom of the jar...

I suppose my question is... what is the most likely reason for his super slow colonization?

He is about to construct a SAB to reduce the contams... he fears that the prints may be contaminated?

Perhaps would be better for him to start with a commercial, or graciously donated :smile: spore syringe/spore print that is less likely to be contam-ed


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Offlinellama_police
Fun guy
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Registered: 02/20/13
Posts: 516
Loc: Australia
Last seen: 8 years, 22 days
Re: Trouble with... well, everything. [Re: junkjunkie2006]
    #19041199 - 10/27/13 02:49 PM (10 years, 3 months ago)

I reckon you should get him to start his own account here and start doing some research.

Or if you're actually referring to yourself in 3rd person, I'd suggest you stop doing it because "SWIM" won't find much help around here.

:notgettingup:


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OfflineMagicMike407
mad scientist
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Registered: 08/17/13
Posts: 201
Last seen: 9 years, 9 months
Re: Trouble with... well, everything. [Re: llama_police]
    #19041246 - 10/27/13 02:58 PM (10 years, 3 months ago)

Could be any of those issues contributing to your troubles. Work on your whole tek so you can narrow it down. If your jars are colonizing slowly i believe its either poor genetics or over-wet grain. Simmer and strain your grain before PC.
Next time try cloning a peice of tissue from a fast colonizer instead of making a suspect syringe or LC, and use that for a master jar for g2g. Thats probably the best way to speed up your times if everything else is ideal.


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OfflineTheGreenBox
Male

Registered: 08/03/13
Posts: 276
Last seen: 4 years, 2 months
Re: Trouble with... well, everything. [Re: llama_police]
    #19041274 - 10/27/13 03:04 PM (10 years, 3 months ago)

Quote:

llama_police said:
I reckon you should get him to start his own account here and start doing some research.

Or if you're actually referring to yourself in 3rd person, I'd suggest you stop doing it because "SWIM" won't find much help around here.

:notgettingup:




QFT


--------------------
And in my darkest moment, fetal and weeping, the moon tells me a secret - my confidant. As full and bright as I am, this light is not my own and a million light reflections pass over me. Its source is bright and endless.

- Tool


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