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Offlinefleki
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Registered: 09/12/13
Posts: 34
Last seen: 6 years, 8 months
Funny pin and some questions about cloning/isolating.
    #19039883 - 10/27/13 09:29 AM (10 years, 3 months ago)

So I'm in the process of my first grow and a mutant has peaked my interest a bit.



As you can see it appears as though there is an albino growing from the pin. My questions are if they were to grow normally would I be able to take the albino portion and isolate it. Also is there any way to maybe preserve a tissue sample for a future grow. My problem is most of the isolation techniques seem to be useful only if you plan to grow continuously and I'm only doing it as needed.


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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
Posts: 380
Last seen: 7 years, 4 months
Re: Funny pin and some questions about cloning/isolating. [Re: fleki]
    #19039896 - 10/27/13 09:32 AM (10 years, 3 months ago)

Yeah pins are great at growing on agar! They explode with growth. You can make a culture slant and store those for the long run, or make a paper pellet tube and inoculate that with tissue. Or store a petri in the fridge. Either will work I am sure. Wait for a second opinion on the matter if you would like


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Invisiblespaceman101
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Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
Re: Funny pin and some questions about cloning/isolating. [Re: nn-IlliniSpiralDMT]
    #19040214 - 10/27/13 10:52 AM (10 years, 3 months ago)

Sorry I'm not trying to hijack but I was kinda wandering about that as well also if I took a tissue sample from the stipe of the mushroom that the little albino was growing from could I make a tub that grew many of these mutations or if I just took a piece off of the little albino on the cap would it grow a lot of albinos of the same nature minus the donor mushroom?


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InvisibleTrentBoyett
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Registered: 11/29/12
Posts: 16,000
Loc: Kazakhstan
Re: Funny pin and some questions about cloning/isolating. [Re: spaceman101]
    #19040637 - 10/27/13 12:44 PM (10 years, 3 months ago)

It might not be albino, maybe it just didn't get its color yet, it just looks likes a primordial knot right now.

But if it is albino then it may or may not produce albino mushrooms when cloned, it's hard to really tell, I mean it should but there's probably also the chance that it'll produce clones that are like the host.


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Offlinefleki
Wanderer

Registered: 09/12/13
Posts: 34
Last seen: 6 years, 8 months
Re: Funny pin and some questions about cloning/isolating. [Re: TrentBoyett]
    #19042455 - 10/27/13 08:03 PM (10 years, 3 months ago)

Oh good call spiral completely forgot about slants I'll be reading up on it =)
Yea it's not an albino, got a bit over excited. Seems just an odd mutation the stem tissue sort of grew through the cap like that. But thx regardless I'll be re-versing myself in the various cloning and isolation techniques before I try anything =)


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