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Invisibleteknix
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Re: Culturing Tech β€’ Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
    #19210272 - 11/30/13 09:33 PM (10 years, 1 month ago)

Cool post, I used a jar and just agar:



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InvisibleWillSolvem
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: nn-IlliniSpiralDMT]
    #19210373 - 11/30/13 10:07 PM (10 years, 1 month ago)

leap off!

They all look good, I  love how clear these PP5  containers are,  sorry for the  shotty pictures,  I'll take more soon!





@nn-IlliniSpiralDMT
it's divided up pretty well,  in spring we inoculate stumps and ground contact logs in the local state parks/forests  for outdoor cultivation while others are cultivated indoors only I usually  don't cultivate  chaga (obviously),  birch polypore, or Turkey tail as the latter two aren't huge sellers and are extremely plentiful here in the northeast.  this year we have moved into 13k sq ft  warehouse to offer year round fresh produce  but still continue the hop houses on the original farm for  seasonal growing. reishi conks are achieved through bag culture, 4.5lbs of  substrate are inoculated with grain,  the bag is closed with two pieces of metal that form a  collar,  the opening is  plugged with polyfil,  after colonized I stack them 10 bags high by 15 bags long (see stamets ggmm "reishi wall"  for those who want to know more) with the opening facing forward, I  secure them with wire and poles that guide the wire, I  wait 48 hrs for  the mycelium to recover then remove the plugs.. you can fit two of these walls in a car  port from harbor freight, I  use solid top plastic shipping pallets for the flooring,  bolt them together,  drill holes for cables as needed,  you get the idea.  there is even space  in the center for two fold out tables for block or bottle cultivation. I  really like the car ports they are very solid once set in concrete,  it defused  the light perfectly,  the entire fabric can be replaced as needed,  if you use buckets of cement or  rocks it can be disassembled and stored as needed.. I  use fabric glue to apply mesh for pest control. and at the price expanding to multiple units is easy and looks uniform,  the down side for a few months out of the year they are useless 


--------------------


AMU Q&A Thread because questions deserve answers.


Edited by WillSolvem (12/01/13 08:51 AM)


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Invisibleanne halonium
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: WillSolvem]
    #19211663 - 12/01/13 09:25 AM (10 years, 1 month ago)

^once again, your not only off topic, but boring.
im sure at this point , youll resort to insults,
in a sad attempt to be relevant,

mostly cuz im rather well known, and you, are not.
insulting me, is the closest youve been to on topic.
and pry the closet you get to glory.
do something of note sometime, and ill care about your opinions.

i scanned your threads.....
im not sure your even an established amatuer.
your comments above, suggest your a new grower, that passed shop class
an established grower would be built out, and not as ego centric .
also,
im glad as an expert grower, you finally discovered PP5 in 2013
we have used grain and PP5 for over a decade.
glad ya finally caught up.


i dont see where this characters shop class grow for edibles, has anything to do with the thread at hand.

i scanned your threads.....
im not sure your even an established amatuer.


Edited by anne halonium (12/01/13 10:00 AM)


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Offlinemastercultivator
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Re: Culturing β€’ STERILE SPORES [Re: RogerRabbit]
    #19211751 - 12/01/13 09:51 AM (10 years, 1 month ago)

Violet.  Awesome writeup especially enjoyed the pics of the pin placed on agar and the pin still maturing into a sporulating fruitbody.  I cut the tops off my pins when I know they are gonna be on agar for more than 5 days because they do try to mature and produce spores.

aeseptic or aseptic is the correct term for 'clean spores'  It means the spores are viable but not contaminated with junk. 

Quote:

RogerRabbit said:
Quote:

Violet said:
STERILE SPORES
Considered an oxymoron by most that I've seen on these forums. It's often said that "No spore print is sterile, since fruits do not grow in sterile conditions." These ones do.




Sterile spores will not grow.  I think you meant to say 'clean spore print' or something similar, but not sterile.  The term sterile spores is only an oxymoron if you expect them to grow.
RR




--------------------
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
I'm awesome sauce with a dash of sunshine.



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InvisiblebodhisattaMDiscordReddit
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Re: Culturing β€’ STERILE SPORES [Re: mastercultivator]
    #19211805 - 12/01/13 10:06 AM (10 years, 1 month ago)

Quote:

mastercultivator said:
Violet.  Awesome writeup especially enjoyed the pics of the pin placed on agar and the pin still maturing into a sporulating fruitbody.  I cut the tops off my pins when I know they are gonna be on agar for more than 5 days because they do try to mature and produce spores.

aeseptic or aseptic is the correct term for 'clean spores'  It means the spores are viable but not contaminated with junk. 

Quote:

RogerRabbit said:
Quote:

Violet said:
STERILE SPORES
Considered an oxymoron by most that I've seen on these forums. It's often said that "No spore print is sterile, since fruits do not grow in sterile conditions." These ones do.




Sterile spores will not grow.  I think you meant to say 'clean spore print' or something similar, but not sterile.  The term sterile spores is only an oxymoron if you expect them to grow.
RR







Yea, I'm going to have to not go with RR on this one. Obviously it's not "sterile" but since there's no word for "only spores and no other material capable of producing some sort of life on a piece of foil etc." we just call it a sterile spore print. Everyone with the slightest hint of common sense would know a sterile SPORE print means there's spores and it's inherently not sterile because of that, but we're obviously not talking about a virgin piece of foil. Clean would be a good word IE clean spore print, but that's more vague and less precise than saying a sterile SPORE PRINT (IE has spores duh).


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InvisibleWillSolvem
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Re: Culturing β€’ STERILE SPORES [Re: bodhisatta]
    #19212080 - 12/01/13 11:10 AM (10 years, 1 month ago)

@anne halonium
Quote:


IΒ  assume I'm dealing with a troll,Β  want to change my mind,Β  post aΒ  picture with my name on a piece of paper next to a living mushroom, take as long as you need



  still waiting on that picture...


Edited by WillSolvem (12/01/13 11:24 AM)


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InvisibleViolet
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Re: Culturing β€’ STERILE SPORES [Re: mastercultivator]
    #19212081 - 12/01/13 11:11 AM (10 years, 1 month ago)

Peeps want to talk about something other than broken-record applications of diverse semantics, edible mushroom farms, egotism defense/attack, sizing-up,  bullshit?


CULTURE TECH thread.


Willsolvem's grain petri posts are the only on-topic part.  Thus it is the only part that may be continued.

Those look great Will!, but it's hard to tell if your lid is screwed down tight.
Since your containers are tall and your grain layer extra shallow, you'll need to be quite careful to minimize moisture loss.  When the time comes to pin them, only slightly crack its lid.



Bodhisatta you see what I mean!
Quote:

I say "sterile spores" in the same way that we do "sterile work" despite that we are actually breaking sterility by inoculating the target organism.
If we did "sterile work" and "kept our jars sterile" as strictly as you imply "sterility" to mean there, we'd never grow anything in our jars either...




--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
β€’ Violet's Teks and Posts β€’


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InvisibleWillSolvem
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Re: Culturing β€’ STERILE SPORES [Re: Violet]
    #19212237 - 12/01/13 11:43 AM (10 years, 1 month ago)

Quote:

Violet said:
Peeps want to talk about something other than broken-record applications of diverse semantics, edible mushroom farms, egotism defense/attack, sizing-up,  bullshit?


CULTURE TECH thread.


Willsolvem's grain petri posts are the only on-topic part.  Thus it is the only part that may be continued.



Loud  and clear,  I'll cut the bullshit out,  my apologies

Quote:


Those look great Will!, but it's hard to tell if your lid is screwed down tight.
Since your containers are tall and your grain layer extra shallow, you'll need to be quite careful to minimize moisture loss.  When the time comes to pin them, only slightly crack its lid.




the lids are on tight,  these are an off brand/store  brand  containers. I only opted for them because they didn't have the indentations on the sides and they were on sale for .99Β’  for three, I  already wish I  bought more!

I have some allenii I just cleaned up for  the 2014 fall season,  I'm thinking of trying it out on this Tek  before I  start my grain masters,  would you know off hand if allenii can be fruited  from Brown rice or would I  need to supplement with sawdust?  does allenii require a casing?  I've never done anything but the flower-pot-woodchip-tek  so I'm limited on my allenii  knowledge


--------------------


AMU Q&A Thread because questions deserve answers.


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InvisibleViolet
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Re: Culturing β€’ STERILE SPORES [Re: WillSolvem]
    #19212265 - 12/01/13 11:49 AM (10 years, 1 month ago)

Oh yeah I know those type store-brand containers, they label them differently for all kinds of chain marts.  They're quite good, the only kind I get other than Ziploc, and I'm happy with them especially for invitro fruiting.


My memory leads me to think Allenii is a seasonal woodeater that calls for the shift of fall to winter conditions.
If that is the case, this culture tech and the container growing tech are probably only limitedly applicable.
Using these containers for agar dishes would still work with any species of course.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
β€’ Violet's Teks and Posts β€’


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Offlinemushrume man
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Re: Culturing β€’ STERILE SPORES [Re: Violet]
    #19212378 - 12/01/13 12:10 PM (10 years, 1 month ago)

I haven't seen these cheaper PP5s. I would love to find them tho since Im a broke SOB.

Could someone post a link or send me in the right direction?


--------------------


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InvisibleWillSolvem
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Re: Culturing β€’ STERILE SPORES [Re: Violet]
    #19212480 - 12/01/13 12:34 PM (10 years, 1 month ago)

Quote:

Violet said:
My memory leads me to think Allenii is a seasonal woodeater that calls for the shift of fall to winter conditions.
If that is the case, this culture tech and the container growing tech are probably only limitedly applicable.
Using these containers for agar dishes would still work with any species of course.




you are correct,  it's a seasonal woodlover.  my thoughts were too mimic the temperatures I  recorded this year to promote fruiting.  I'll grab some more containers and try some varied  media.  it could be a waste of time by my search results rendered only theories but no similar applications so somebody should try it,  even if all we learn  is that it doesn't work,  I'll keep you updated


--------------------


AMU Q&A Thread because questions deserve answers.


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InvisibleHypnotoadCroaked
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: anne halonium]
    #19215899 - 12/02/13 04:38 AM (10 years, 1 month ago)

Quote:

anne halonium said:

mostly cuz im rather well known, and you, are not.







You are guilty of being a hero in your own mind.  Your horrible attempts to "promote" your future WHILE you are murdering sentence structures does not impress.  You may be well known, but its not for the reasons you seem to think.  Infamous/famous are two different things.

Thanks for your whatever it is you brought to the table Anne, but I have given you enough of my time.  I feel dumber for reading your posts, and will be ignoring you.  You come preaching about the future, but the only solid thing you have presented about said future is PP5 containers.  Myself and others around these parts discovered pp5 long before you ever came here telling us how wonderful it was.  TBH, this forum would be better off without you, but that is my opinion.


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InvisibleViolet
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Re: Culturing β€’ STERILE SPORES [Re: mushrume man]
    #19216221 - 12/02/13 07:40 AM (10 years, 1 month ago)

Excuse me, but what does that have to do with Culture Tech Thread?
Did someone ask you to post your opinion of Anne here?  That wasn't even worth your mental time or typing energy.
Especially not posting a single thing relevant to the thread.  I'd even say that garbage is plenty more annoying.
I said no more of both that type comment from Anne as well as if not Especially yours.  I don't give a damn how badly you want to get your shot in.  Get over it / grow up and drop it,  or retain it and stew over it at OTD,  but this is not the place.
You'll only irritate the moderators.  And rest assured they Will be notified of any more off-topic, personal attack or flaming, egotistical sizing-up, rule-breaking,  wrong-forum bullshit.  I have lost all patience for that over the last year.


Quote:


mushrume man said:
I haven't seen these cheaper PP5s. I would love to find them tho since Im a broke SOB.
Could someone post a link or send me in the right direction?



They're really not that much cheaper, if at all.
All kinds of stores carry these type re-brand containers, for instance stores like kmart & target, and local grocery marts


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
β€’ Violet's Teks and Posts β€’


Edited by Violet (12/02/13 07:50 AM)


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Invisibleanne halonium
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: HypnotoadCroaked]
    #19216320 - 12/02/13 08:24 AM (10 years, 1 month ago)

Quote:

Whippy said:
Quote:

anne halonium said:

mostly cuz im rather well known, and you, are not.










im not interested wipey,
in giving you a platform for your trollery on this thread.
PM me , and let me know who you are so i can care?


please, on with the culture tek show.


--------------------
:aliendance:


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Invisibleblindingleaf
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: anne halonium]
    #19218018 - 12/02/13 04:21 PM (10 years, 1 month ago)

sry took a bit to upload.  so, my last post talked about how the grains might have been too dry...guess not.  the wheat doing ok too, but only has spread to one grain, so this is only worth pic.  guess I'm gonna drop agar wedges in the other 5.  the rubber band is on there because its a glad snap on and its a bit too loose for my taste.  i don't band up my agar PP5s made exactly same way, but grains I do cause the agar plates get used within 4-5 days, where these will go ......1-2 weeks....?  thats only 3 tbs BR, so maybe a week?

BR:


Agar



Question:  about peat moss.  I live in the city and own a bike.  The closest thing to a chain I have is a 35 min bike ride to get peat/jiffy from home depot/target/etc.  the local pet store has peat moss in a gallon zip lock bag (I guess the guy bags it himself..?)  it literally looks like green moss.  can I use this to case the little grain jars (with verm, pasteurized) or should I go for the gold? (just don't wanna bike a big bag of peat/jiffy back home, but I will if I have to)


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinemushrume man
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: blindingleaf]
    #19218586 - 12/02/13 06:22 PM (10 years, 1 month ago)

So I just got done making eight agar pp5s. And one pp5 of distilled water for myc suspension.

My first attempt at scraping myc from agar was a bit clumsy. I did end up w some floating in the water but it was chunky so I shook it hard and it eventually sprayed water all across my walls and floor. Just a warning to everyone these things CAN leak.

Not sure if my suspensions soiled but I will test it w a dish I just made:syringe:


--------------------


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Invisibleanne halonium
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: mushrume man]
    #19218703 - 12/02/13 06:43 PM (10 years, 1 month ago)

good report.
its a learning curve with PP5.
for most all purposes, PP5 is considered " no shake"


--------------------
:aliendance:


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Invisibleblindingleaf
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: mushrume man]
    #19218717 - 12/02/13 06:45 PM (10 years, 1 month ago)

can't u just drop an agar wedge into the pp5 of water or do u have to scrape the myc off?  or maybe let the pp5 grow out a bit, squirt in some clean water, swish it around to get the myc off the agar, then suck up again to use/store? 

I've only done an LC once, and all I did was drop a single grain from a 100% WBS jar into honey water that was PC'd.  u probably have done it way more than me, but thats my idea/experience.

now that I said that...I just had an idea/question...
what if u used the PP5 agar and let it grow out 75%.  then put X amount of sterilized water in, say 50 ml.  can that be used continuously until the 50 ml is gone?  meaning each time u want to use the water to noc up a jar, just swoosh it around till some myc is floating, then get 2-4 cc's out then put back and do again when u wanna noc up more jars later (so if u had 50ml and used 2ml for each innoc. u could theoretically use it 25 times)?  will the solidified agar at the bottom act as a (delayed?) nutrient base and keep the myc growing or will this idea just not work at all?  so I guess Im asking, could the violet PP5 agar petris be used/converted into an LC/suspended myc container?  like I said LC's are very foreign to me, so I haven't done much reading on them.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Invisibleanne halonium
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: blindingleaf]
    #19218729 - 12/02/13 06:47 PM (10 years, 1 month ago)

lets see what violet says on this topic.

i use polycarbonate at that phase of ops for slurry.
i dont want to wander to far .


--------------------
:aliendance:


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InvisiblePastywhyteMDiscord
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Re: Culturing β€’ GRAIN 'PETRI' TEK [Re: blindingleaf]
    #19218732 - 12/02/13 06:48 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
or maybe let the pp5 grow out a bit, squirt in some clean water, swish it around to get the myc off the agar, then suck up again to use/store?




I did this a year or so ago. Not sure how reliable it is overall but I got clean growth with mine. It actually picked up some of the nutes and green tint from my colored agar and actually colonized the syringe quite thick.


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