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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#23088541 - 04/06/16 01:16 AM (7 years, 9 months ago) |
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Well I know I'd love to see any additions/alterations as you develop them...I'm not too scared of new ideas to try it Maybe they'll let you post your 'crazy ideas' in your journal?
Thanks For everything
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Psilosopherr]
#23088880 - 04/06/16 06:41 AM (7 years, 9 months ago) |
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Violet you know I enjoy your posts. I missed most of the drama but went back and read for myself. I do think you were dealt perhaps harsher than let's say a bald faced noob coming in but, I also think that cultivators at your level need to be held to a higher standard as well.
I tried a few runs with my microwave, brf and mea. All ended with bacteria. I think I need to hold a boil much longer due to my elevation (2200ft). As such I will also need to start with far more water in my mix.
Microwaves are a touchy subject and for good reason. They are something that can go sideways pretty easy IME. I know I am nervous running stuff in em for long periods. To be honest they freak me out a bit. My suggestion is that any future attempts/writeups be well disclaimered, while any defense of said ideas be done carefully.
Good to see you back tho
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Pastywhyte]
#23089396 - 04/06/16 09:59 AM (7 years, 9 months ago) |
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But I'm not back, not really.
"Perhaps dealt harsher"? Well man there's quite a lot that you weren't able to have "went back and read". That shit was laden with vendetta, double-standard, and posturing. It's a bit of a big accusation, perhaps, but let it be said at least once that it was in some fashion a coup.
There's nothing scary about microwaves. It just takes understanding them, and right now the cultivation community is ignorant about its own ignorance about microwaves as a heat source. It just takes people doing the grind to sort out how to go about things in such a different method of heating. I even have a tek for "traditional" pasteurization that's fairly simple, stowed away for the fine day that there's a community that's simultaneously active AND that doesn't blow balls.
Sorry yours didn't do too well. What I had found was that my success rate against bacteria with the recipe as it is now locked in that thread was fairly media-dependent, and somewhat effected by the container and its use as well. That's why I wish I could edit the thread and continue developing *UNDERSTANDING* about this, either by myself or collaboratively. But that's is just another surfacing symptom of the egotistical repression of developmental growth in this forum, and it doesn't seem like anybody gives a fuck about doing anything about that, so, I'm gone.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet] 1
#23090484 - 04/06/16 03:56 PM (7 years, 9 months ago) |
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I wonder if you could microwave agar in a closed PP5 container if the lid had a layer of ice cubes on it to condense the steam.
According to the internet microwaves don't effect ice https://answers.yahoo.com/question/index?qid=20100128120806AA30i67
Probably just a wacky impractical idea but I thought I'd mention it
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Jerome09
Alchemist


Registered: 02/27/15
Posts: 28
Last seen: 6 months, 3 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Psilosopherr]
#23115315 - 04/13/16 11:06 PM (7 years, 9 months ago) |
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Nooooo VIolet you are the best!!!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#23115375 - 04/13/16 11:27 PM (7 years, 9 months ago) |
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Quote:
Violet said: But I'm not back, not really.
"Perhaps dealt harsher"? Well man there's quite a lot that you weren't able to have "went back and read". That shit was laden with vendetta, double-standard, and posturing. It's a bit of a big accusation, perhaps, but let it be said at least once that it was in some fashion a coup.
There's nothing scary about microwaves. It just takes understanding them, and right now the cultivation community is ignorant about its own ignorance about microwaves as a heat source. It just takes people doing the grind to sort out how to go about things in such a different method of heating. I even have a tek for "traditional" pasteurization that's fairly simple, stowed away for the fine day that there's a community that's simultaneously active AND that doesn't blow balls.
Sorry yours didn't do too well. What I had found was that my success rate against bacteria with the recipe as it is now locked in that thread was fairly media-dependent, and somewhat effected by the container and its use as well. That's why I wish I could edit the thread and continue developing *UNDERSTANDING* about this, either by myself or collaboratively. But that's is just another surfacing symptom of the egotistical repression of developmental growth in this forum, and it doesn't seem like anybody gives a fuck about doing anything about that, so, I'm gone.
i offered to move it to your journal
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: cronicr]
#23116589 - 04/14/16 10:46 AM (7 years, 9 months ago) |
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in terms of microwaving, breast pumping equipment come with a steam sterilizer type contraption that you can microwave. it heats up the water and steam sterilizes the plastic inside the microwave, so it says. i wonder if that would be something useful in sterilizing things in the microwave?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: eatyualive]
#23116650 - 04/14/16 10:59 AM (7 years, 9 months ago) |
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they also have microwave pc's lol....wtf!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: cronicr]
#23116664 - 04/14/16 11:04 AM (7 years, 9 months ago) |
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I know i just saw a post about those the other day! idk how i would feel about that.. but from what i could tell, it seemed more like a steamer, rather than a real PC.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: mupetmower]
#23116901 - 04/14/16 12:41 PM (7 years, 9 months ago) |
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cronicr for whatever reason I don't really recall that. Moving it to my journal and unlocking it would be a good thing to do for it. If you find a moment, please do that; I would appreciate it.
Yeah I've seen the little "pressure cookers" for microwaves. They do maintain water better and thus will build some pressure, also sterilizing a bit more thoroughly and faster. I haven't used one though - they look like they could hold just one container at a time. I know for a fact that can be done, but it also kindof defeats the purpose of finding out how to just use the microwave. Also am unsure how it might change the dynamic of using the mic - would using that require closer to the longer times PCs take, expos even longer due to pressure as low as 3-6 PSI, losing the microwave's advantage?
I might pick one up sometime just to learn more. But the real work to be done is figuring out the right way to use the microwave alone for everyday applications
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#23117008 - 04/14/16 01:14 PM (7 years, 9 months ago) |
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I think even if there are ways to make a microwave work, it just seems that having a PC would be more beneficial for most things, and seeing as you can find them for $20(mine is 8 quart, $20 brand new), it isnt all that much money if you intend to stay with this hobby. But, if there are ways to make a microwave wrk, then by all means. I am just trying to point out the fact that there really isnt that many reason why someone cant get a PC, unless its just because they simply dont want to for whatever reason. I do think that finding new ways to do things is a good thing, however. so for that reason, i will probably come back to this thread to see if you have made any progress.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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JoeLowPro
Stranger
Registered: 02/14/17
Posts: 6
Last seen: 5 years, 5 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#25060999 - 03/13/18 04:19 PM (5 years, 10 months ago) |
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Violet, Thanks for all the knowledge you share.
Do you have any advice on culturing stone producers? What type of pitri substrate? I was thinking grain water pda but now thinking maybe rye berry Petri. Am I correct in understanding that grass seed is preferred for cubes but rye berries for stones?
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lilwoman
Stranger
Registered: 05/03/19
Posts: 56
Last seen: 2 years, 2 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#26119901 - 07/20/19 01:20 PM (4 years, 6 months ago) |
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Excellent use of waste!!
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bongoman
Stranger

Registered: 09/19/18
Posts: 558
Last seen: 6 days, 13 hours
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: lilwoman]
#26129908 - 08/10/19 03:20 PM (4 years, 5 months ago) |
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Revisiting the sterile spore print method.
I imagine if I sterilise tin foil in a PP5 container that it will be quite wet after coming out of the pressure cooker.
What might be a way to dry it sufficiently before printing? Is it enough to leave it in the SAB?
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Izzamagic
Stranger
Registered: 04/20/20
Posts: 72
Last seen: 1 year, 8 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: JoeLowPro]
#26717806 - 06/03/20 09:11 PM (3 years, 7 months ago) |
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Quote:
JoeLowPro said: Violet, Thanks for all the knowledge you share.
Do you have any advice on culturing stone producers? What type of pitri substrate? I was thinking grain water pda but now thinking maybe rye berry Petri. Am I correct in understanding that grass seed is preferred for cubes but rye berries for stones?
I'm also interested to learn about culturing stone producers.
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ronswanson42069
Stranger
Registered: 12/12/20
Posts: 5
Last seen: 2 years, 5 months
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Violet]
#27320419 - 05/23/21 07:14 PM (2 years, 8 months ago) |
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I just cased my brown rice ziploc invitro container using your casing tek. Will the pins still be sterile enough to pluck right off the casing layer and put directly on agar, or did I ruin the sterility of the pin by opening the container to put the soil casing on the rice? Do I need to cut into a mushroom to get cleaner material like other teks now?
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gun fuy
Stranger
Registered: 10/22/21
Posts: 20
Last seen: 3 days, 8 hours
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Violet]
#28015728 - 10/25/22 10:38 AM (1 year, 3 months ago) |
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This is super interesting and it's what I intend on playing with once I learn agar, which seems like a more important first place to start. Currently just gathering materials and hesitating between poured glass petris or no-pour screw-top containers. I think I'll do both.
A question comes to mind: Would you be able to do "Grain Petris" in actual petris rather than in no-pour style PP5 containers? eg. Wrap a stack of them in foil in the PC?
My understanding is that you can't really do no-pour agar in petris because they're so shallow and pretty much guaranteed to overflow. Would that problem (or others) occur with a thin layer of grain in the petri?
-------------------- "Most multispore projects will be more productive than most isolates. The differences is a FEW isolates will be killer, and those are the ones you want to save." - RR
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grain
Stranger



Registered: 07/16/22
Posts: 35
Last seen: 8 months, 28 days
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Violet]
#28015770 - 10/25/22 11:30 AM (1 year, 3 months ago) |
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I do no pour agar petri dishes all the time. They don't overflow from my experience. I use painters tape (blue tape 1.5 inch wide) to hold the stack together. I make an "x" of tape on the table, put the first dish centered on the x and do stacks of 10. Then just bring the ends of the x up and over the top. Then I remove the stacks while hot and put another x of tape so the sides of all the dishes are completely covered with tape. I like it cause I can cut as many dishes off the top of the stack leaving the remainder still sealed with tape. I don't parafilm or seal them individually. They are in the tape ready to use.
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gun fuy
Stranger
Registered: 10/22/21
Posts: 20
Last seen: 3 days, 8 hours
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Re: Culturing • GRAIN 'PETRI' TEK [Re: grain]
#28029573 - 11/02/22 03:05 PM (1 year, 2 months ago) |
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Thanks for the reply, noted to try that at some point. But my question was more geared toward doing this grain 'petri' in actual glass petris instead of in plastic containers.
Is the reason Violet chose the containers because she already uses them for agar (which would be no-pour) ie. convenience? or could you just put a thin layer of grain in an actual 100mm glass petri dish to do this ?
-------------------- "Most multispore projects will be more productive than most isolates. The differences is a FEW isolates will be killer, and those are the ones you want to save." - RR
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