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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing • INVITRO GROWING [Re: KauaiOrca]
#21929390 - 07/11/15 03:43 PM (8 years, 6 months ago) |
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That's what I typically do, as I settled into a bit more of a routine with these methods. The brown rice gives you a bit more yield potential per container, though you have to let it sit longer to produce its whole potential. Grass seed both fruits and flushes out significantly more quickly due to being lighter and fluffier, so for quicker container turnaround it would be better. But I don't have a need for that. I'm happier throwing an easy microwaved soil casing on there and letting it ride out for however long. But quick cultures, found with this method, can darastically speed even that process up.
I don't flour the rice. To do that, you have to mix it with verm. That's a great method for beginners, especially those without a pressure cooker where I consider it the best method for beginners. But when you get the swing of the method, especially with great cultures as per this method, whole grains are both easiest and have the highest yield potential.
I'm glad you asked that, about the strain (variety). My first impulse, and the politically correct one around here, is to say there's no effective difference. But I gotta be real. It should be much easier finding a culture to excel prolifically in cultivator conditions when the spire variety it came from is something that's highly domesticated. One of the bigger early culturing runs was done with "Shooting Star", and though I did get a pretty great culture from it, I have done notably better with Golden Teacher and Albino A+. Something like those are likely to be winners. Think those, Treasure Coast, PE6, Cambodian... I hear great things about some others too. Many growers on here have some sweet Wikizdon grows that show lots of potential from it.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Culturing • INVITRO GROWING [Re: Violet]
#21929601 - 07/11/15 04:28 PM (8 years, 6 months ago) |
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Quote:
My first impulse, and the politically correct one around here, is to say there's no effective difference. But I gotta be real. It should be much easier finding a culture to excel prolifically in cultivator conditions when the spire variety it came from is something that's highly domesticated.
I will agree with this except I will add that the care an lineage of the variety has a lot to do with it as well, even after domestication. I had made a thread a few weeks ago that touches on these topics a bit, some good non dogmatic discussion in there.
http://www.shroomery.org/forums/showflat.php/Number/21774758
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KauaiOrca
Waterman


Registered: 08/12/08
Posts: 3,131
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Re: Culturing • INVITRO GROWING [Re: Pastywhyte]
#21929673 - 07/11/15 04:44 PM (8 years, 6 months ago) |
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Quote:
Pastywhyte said:
Quote:
My first impulse, and the politically correct one around here, is to say there's no effective difference. But I gotta be real. It should be much easier finding a culture to excel prolifically in cultivator conditions when the spire variety it came from is something that's highly domesticated.
I will agree with this except I will add that the care an lineage of the variety has a lot to do with it as well, even after domestication. I had made a thread a few weeks ago that touches on these topics a bit, some good non dogmatic discussion in there.
http://www.shroomery.org/forums/showflat.php/Number/21774758
Thanks for the suggestion. The first time I tried growing, I had incredible results with invitro bags. I had 4 different multispores syringes I turned into LC's, and one of them was incredible … bag after bag just packed with fast growing mushrooms that wanted to climb out of the top of the bags. I thought growing was the easiest thing in the world. Never got into cloning or cultures, just kept using that same LC. Then, a few years later, got some new spore syringes and could not get anywhere near the same results with the exact same bags. Nothing changed, (except the LC, of course) but the results sure did. Now I'm trying to somehow capture that same magic in a new strain/culture that will grow fast and effortlessly invitro. What attracted me initially to the V-Tek system was how easy culturing and cloning is with the PP5 Jars and RGS … Hopefully, I'll find one that really works in a reasonable amount of time.
-------------------- "The universe is endless, limitless and infinite. Any effort to define it's boundaries is an attempt to overcome ignorance. We are physical, mental and spiritual beings ... there is no beginning and there is no end. There is only memory. Our repeated loss of memory experiences create the illusion of beginnings and ends. Immortality is the ability to retain full memory through all consciousness transformations. Loss of memory is man's greatest curse and, in very real terms, death." -- Ancient Taoist Master
Edited by KauaiOrca (07/11/15 04:45 PM)
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durxo
Stranger



Registered: 01/16/14
Posts: 113
Last seen: 2 years, 1 month
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#21957190 - 07/17/15 05:44 PM (8 years, 6 months ago) |
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Nice Tek, thx man
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“The psychotic drowns in the same waters in which the mystic swims with delight.” ― Joseph Campbell
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Sanchez333
Stranger
Registered: 06/22/15
Posts: 27
Last seen: 3 years, 10 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: durxo]
#22042853 - 08/04/15 04:06 PM (8 years, 5 months ago) |
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I'm just realizing I was suppose to freeze my grain water. I poured it into canning jar and straight to fridge about week and half ago. Bad idea to try and use?
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Sanchez333]
#22044853 - 08/04/15 10:06 PM (8 years, 5 months ago) |
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I freeze it when I think it will be a long time before I use it. Honestly I got out of that habit because of how frequently I prepared grains. Instead I refrigerated them for a while. You just don't want them to get a big stinky bacterial bloom, which consumes nutrients and can change pH. Might should mention before the freezing option that use in the near future would warrant refrigeration. 9-12 days in refrigeration is likely fine. Just smell of it and see if it's starting to get rank.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#22044874 - 08/04/15 10:10 PM (8 years, 5 months ago) |
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been awhile
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Sanchez333
Stranger
Registered: 06/22/15
Posts: 27
Last seen: 3 years, 10 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: cronicr]
#22068667 - 08/09/15 06:20 PM (8 years, 5 months ago) |
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Do you have to store unused "Petris" in fridge till inoculated?
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Sanchez333
Stranger
Registered: 06/22/15
Posts: 27
Last seen: 3 years, 10 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Sanchez333]
#22075556 - 08/11/15 09:28 AM (8 years, 5 months ago) |
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I have quite a bit of condensation on lids of containers after being pc prob not good?
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Sanchez333]
#22075921 - 08/11/15 11:04 AM (8 years, 5 months ago) |
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When a plate has grown out long enough to be assured clean, and I don't plan to use it for more than a week, I do fridge store them. Otherwise they'll get over-grown. Remember we want the leading edge for transfers, though inner growth is fine/great for inoculations of grow outs.
Condensation is common and normal. Personally I shake/pour it out before inoculation, but I have an essentially risk-free sterile airflow cabinet. It is less advisable in a still air box, but pouring some out can be helpful and low-risk. Note that I'm not advising it as part of procedure, especially for beginners. But when you get the gist of it, you can choose to do it and see if it happens to make your life easier.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Sanchez333
Stranger
Registered: 06/22/15
Posts: 27
Last seen: 3 years, 10 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#22076486 - 08/11/15 01:45 PM (8 years, 5 months ago) |
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Can the drops of water off lid hurt the growth? Reason for asking I went ahead and noc them up with condensation my dumb ass was to impatient for your reply my bad.
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Sanchez333
Stranger
Registered: 06/22/15
Posts: 27
Last seen: 3 years, 10 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Sanchez333]
#22146946 - 08/26/15 02:47 PM (8 years, 5 months ago) |
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Can you store fully colonized Petris in fridge for later use?
Edited by Sanchez333 (08/26/15 02:48 PM)
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Sanchez333]
#22177447 - 09/02/15 09:20 AM (8 years, 4 months ago) |
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Sorry for the delay! Missed these.
Water shouldn't hurt them unless a large amount of it pools. If there's a risk of bacterial contamination (you'd have to transfer away from it anyway) water pooling can help it spread so much that it can be beyond recovery. Still it's nicer not to have all the excess water.
I can and do store these dishes in the fridge! It effectively suspends their growth. However, over extra-long term it allows the agar itself to shrivel up with moisture fluctuation just like a petri dish would, albiet slower and the dish retains that water, just in the wrong place as condensation. I have some that have been in the fridge for a year and a half. The agar is not looking so great, but I feel fairly confident that the myc can be revived with little issue!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#22278097 - 09/23/15 10:14 AM (8 years, 4 months ago) |
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Apologies for resurrecting your thread, but I figured you'd prefer I post here over a 
I've been trying to find the perfect pair of forceps for grass seed petri transfers. (harder than one may expect at first glance.)
So three purchases later I've found a usable pair, but I'm wondering what kind you use? Do you use classic metal tweezers or the kind with scissor handles?
The pair that worked best for me was a variety pack of tweezers from the dollar store of all places. For me the forceps with scissor handles take far too long to get red hot (even after buying a smaller pair)
The problem is finding a pair that is a thin enough metal to get red hot nice and fast; but it also needs to be "heat resistant" enough that I'm not burning my fingers trying to hold the thing.
But yeah, whats your recommendation violet? Ever run into this issue?
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Psilosopherr]
#22278261 - 09/23/15 10:59 AM (8 years, 4 months ago) |
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I don't remember where it came from but I've just got a good pair of tweezers, longer than the ones that come in little hygiene kits. Also, I bought a rather long one, on eBay! You can find them pretty cheap on there! You only have to wait for the first signs of being behind hot enough to turn red on the outside when sterilizing metal so thick. With syringes it's important for both the inside and outside and the metal is very thin; but the outside of something like tweezers should be sterile before the metal appears fully red.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#22278297 - 09/23/15 11:07 AM (8 years, 4 months ago) |
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very good to know, thanks 
I had heated a thick pair all the way red and it turned into a burnt/caked on mess, complete with a smell identical to popcorn.
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Jerome09
Alchemist


Registered: 02/27/15
Posts: 28
Last seen: 6 months, 3 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Psilosopherr]
#22878667 - 02/08/16 11:29 AM (7 years, 11 months ago) |
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Does the grain Petri tek work for truffle species??
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Jerome09
Alchemist


Registered: 02/27/15
Posts: 28
Last seen: 6 months, 3 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#22914957 - 02/17/16 09:33 AM (7 years, 11 months ago) |
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Hi VIolet,, what do you mean when you say "these cultures are and will continue to be more healthy and vigorous." in reference to cloned small pins on agar..
They will continue to be more healthy as they spread out further along the agar? or...
I'm trying to figure out how cultures get more healthy.. I'm in the process of trying to revive an old ms syringe culture I have lying around. I've never worked on culturing before just took whatever growth I got and tried to fruit it. Now I realize culturing is the way to go. Excited to transfer grains and pins from your grain petri tek to some of your microwaved agar plates and start selecting rhizomorphic growth and more importantly big pins from big clusters. Still feel like i'm missing something about the culturing process which is why I asked the above question. Hopefully I will understand more once I get some first hand experience culturing.
Thanks violet
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
#23088511 - 04/06/16 12:58 AM (7 years, 9 months ago) |
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I'm quite pissed that your thread about microwave agar got locked, seemed totally uncalled for. So attached to their fancy methodology that they can't let low tech teks exist? fuck me, that should not be what we're about.
Anyway, had a question, has WBS been used for grainwater agar with success? Only saw mention of grass seed and brown rice, figured it'd work the same but why not find out.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Psilosopherr]
#23088523 - 04/06/16 01:04 AM (7 years, 9 months ago) |
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Yeah, that was legitimately uncool. People playing games. It was a political execution of sorts - or part of a larger one.
What upsets me is that I cannot edit it now. I've refined it a little bit, to make the more secure (such as a longer treatment time and an adherence to lower power levels for longer times when a rush is of no importance), and have a few recommendations regarding media and containers that benefit the success rate. Suffice it to say that Machiavelliavore was onto some stuff that turned out quite valuable as additions to the method.
But somebody wanted to stunt the growth of the community to bolster their egos. That's people, though...
Yeah birdseed grainwater works fine. Also I made some birdseed water agar to give it a go with the microwave prep, and it worked nicely for me. Better success rate with short treatments than brown rice, which I would no longer heavily recommend for that method.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
Edited by Violet (04/06/16 10:15 AM)
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