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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: invitro]
#20626735 - 09/27/14 04:37 PM (9 years, 4 months ago) |
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anything made from polypropylene(PP) or with the recycling code 5
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: invitro]
#20627985 - 09/27/14 09:14 PM (9 years, 4 months ago) |
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Quote:
invitro said: Can someone share with us the dimensions of the different twist n loc containers? They come in various shapes and sizes and the specifics are amazingly hard to find. I can't get these locally, they don't carry them.
Even ziploc.com doesn't have the information and their customer service center flatly refused to provide the information. I don't know weather to laugh or write them a nasty letter, is it a secret?
That's pretty ridiculous.
There are 2 containers by ziploc. One is pint 16oz, the other is quart 32oz. Both have the same size base and the same size lid. One of them is just twice as tall.
I use them constantly and never made a point of finding out the exact dimensions. Never needed them for anything... I just use the damn things!
Ziploc containers are immensely recommended. I wish they had perfectly round sides, and clear lids, but they're still notably better than any of the several others I've tried
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20628004 - 09/27/14 09:20 PM (9 years, 4 months ago) |
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life brand makes one with perfectly round sides and a more transparent lid but they do feel a bit cheaper.
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#21020254 - 12/24/14 06:53 AM (9 years, 1 month ago) |
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Just subscribing to the thread. Do not write me or quote my message. If you feel the need to talk to me then PM me or I will PM you. I also hang out in jounals. I refuse to post on the board. I am just subscribing to this thread.
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Guardian187
Neophyte


Registered: 11/29/14
Posts: 716
Loc: Center of the Universe
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Re: Culturing • GRAIN 'PETRI' TEK [Re: blackdust]
#21163285 - 01/22/15 02:14 PM (9 years, 8 days ago) |
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Great writeup. I'm very anxious to isolate cultures on agar and test their fruiting.
I cannot believe the amount of trolling on this thread! Incredibly annoying having to read through so much shit to see what additional on-topic comments were made.
What other methods are typical for isolating and testing cultures? I'm still working on petris at the moment, but I do want to test isolations effectively, and this seems to be the best tek I've found so far.
Open to other suggestions so I can compare and contrast
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blackdust


Registered: 02/28/09
Posts: 8,327
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Well, space came up with a good idea for me and I will be making an iso with this method. Grain water and agar-agar. may add a lil somthen special to the mix though. Shhhhh
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Guardian187
Neophyte


Registered: 11/29/14
Posts: 716
Loc: Center of the Universe
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Re: Culturing • GRAIN 'PETRI' TEK [Re: blackdust]
#21164088 - 01/22/15 05:49 PM (9 years, 8 days ago) |
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Quote:
blackdust said: Well, space came up with a good idea for me and I will be making an iso with this method. Grain water and agar-agar. may add a lil somthen special to the mix though. Shhhhh
Lol
I'll be monitoring
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GoodyMcLuvin
Shamanic Level Dimitri Proponent


Registered: 01/12/15
Posts: 74
Loc: Hippie Town, NC
Last seen: 8 years, 4 months
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Guardian187]
#21189190 - 01/27/15 10:20 PM (9 years, 3 days ago) |
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Ya the trolling on Vs threads is ridiculous. "do you always go grain petri to agar, so you know you're working with a fruiting substrain then go to the agar to look for rhizo growth on the agar, or do you believe grain Petri's could replace agar work in the future for HOME mycology by being sufficient for strain isolation?" OK ,V this is the question I asked on the other thread. Eventually I found a similar question and more or less your answer broke down to a no because we need agar's 2D surface. Thank you once again, for the luminescence which is Violet. And I read where you said you were growing edibles for the farmers market and some local supermarkets... I swear on everything that's exactly what I had intended on doing. Is it going well? I love in Asheville North Carolina, it's a very artistic, organic, vegan city. I believe I'll do well here...
-------------------- *I graduated from the corner, my respect be my diploma,I went OG for the dollars, Js thought I went to college*
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Rhizo structures have very little to do with fruiting potential if anything at all, and are merely indicative of colonization speed. Even then its more media dependent than anything and given that some of the fastest and most aggressive species we know of in mycology are pretty much incapable of forming a rhyzomorphic structure its significance needs to be taken in context of the other variables in play.
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Pastywhyte]
#21189312 - 01/27/15 10:46 PM (9 years, 3 days ago) |
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Quote:
Pastywhyte said: Rhizo structures have very little to do with fruiting potential if anything at all, and are merely indicative of colonization speed. Even then its more media dependent than anything and given that some of the fastest and most aggressive species we know of in mycology are pretty much incapable of forming a rhyzomorphic structure its significance needs to be taken in context of the other variables in play.
Perfectly worded.
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GoodyMcLuvin
Shamanic Level Dimitri Proponent


Registered: 01/12/15
Posts: 74
Loc: Hippie Town, NC
Last seen: 8 years, 4 months
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But after finding a fruiting strain on the grain Petri's, going to agar to look for rhizo growth would be the next step wouldn't it? Or is the sectoring more important? Because we established fritting strains with the grain petri... Right?
-------------------- *I graduated from the corner, my respect be my diploma,I went OG for the dollars, Js thought I went to college*
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GoodyMcLuvin
Shamanic Level Dimitri Proponent


Registered: 01/12/15
Posts: 74
Loc: Hippie Town, NC
Last seen: 8 years, 4 months
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You keep talking like that, and we'll be more than just friends...
-------------------- *I graduated from the corner, my respect be my diploma,I went OG for the dollars, Js thought I went to college*
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
GoodyMcLuvin said: But after finding a fruiting strain on the grain Petri's, going to agar to look for rhizo growth would be the next step wouldn't it? Or is the sectoring more important? Because we established fritting strains with the grain petri... Right?
You have only established a fruiting strain once you see a fruit. If a pin pops up and you clone it then the odds are in your favor. But given that with a flour petri (which is what I assume you are referring to) you cannot see sectors well it is possible that you strip out and isolate a poor performer and will not get good results.
The plate below is two cultures cloned from the same invtro pin. The line of separation is clear despite the fact that they came from the same fruit. The weaker culture fruited poorly and an inoculation of both in the same jar was attempted with poor results. Meanwhile the stronger culture on its own, did fruit quite well.

There are some who believe that recombination or anamostosis means that some trace genetics from all strains will even be present in all or at least most sectors from cloned fruits, however I do not take this to mean that similar performance can be expected. On the contrary my experiences have tended to indicate the opposite.
We first look for strong healthy growth simply because it is initially the most desirable trait. We worry about the rest later. Chances are that you will not find a non fruiting strain. While poor performers are common I have myself only found truly non fruiting genetics a handful of times.
Edited by Pastywhyte (01/27/15 11:11 PM)
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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 7 years, 10 months
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After your initial sample.... Grow that out on agar... isolate each sector to its own dish until you have actual isolates...
then test each isolate for all desired qualities.
Keep the good one/ones.
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GoodyMcLuvin
Shamanic Level Dimitri Proponent


Registered: 01/12/15
Posts: 74
Loc: Hippie Town, NC
Last seen: 8 years, 4 months
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Mushroom_J]
#21189442 - 01/27/15 11:27 PM (9 years, 3 days ago) |
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OK. And isn't a flour petri made from brf paste? Thank you all so much. I started growing in 2012, and was a member here. I started off doing brf and was. I went straight to trying to do agar. I used PDA, ordered agar and boiled the potatoes myself. Couldn't get the spores to germinate on the agar, but succeeded in getting a multispore inoculation of wbs to colonize and made some transfers. Of course I was transferring the linear mycelium, something has gotten lost in the endorsing. Well during all that I had a run in with the law, and ended up going to jail for ten months. In just now getting back into it. And FYI I went to jail on a sell or delivery. In not a burglar. Lol. But thanks so much for the help. I plan on being here to stay.
-------------------- *I graduated from the corner, my respect be my diploma,I went OG for the dollars, Js thought I went to college*
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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 7 years, 10 months
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Yea a flour petri would be like brf paste...
The point of the "grain" petri is that the grain provides the fuel need to throw out decent specimens.. clusters...
Just agar may throw out a pin or two.. which says yea this strain has fruiting potential.. but doesn't indicate how much...
The grain petri allows you to skip a bunch of isolations by allowing pin clusters to form so you can take multiple clone samples (pins) and grow them out on agar...
Instead of putting spores to agar... then transfer... more transfers... then more transfers... then even more....thenm more lol.. then having to trst each isolate... that's alot of fuckin work lol...
skip all that shit and clone pins from clusters...
I'm sure violet would explain it better lol
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GoodyMcLuvin
Shamanic Level Dimitri Proponent


Registered: 01/12/15
Posts: 74
Loc: Hippie Town, NC
Last seen: 8 years, 4 months
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Mushroom_J]
#21189492 - 01/27/15 11:43 PM (9 years, 3 days ago) |
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Thank you so much!! You both rock. And yes, V def has a way with words. She's not afraid to break it down to the hoi palloi, such as myself. But thanks so much. Any tips besides use the search bar, follow the teks, and don't improvise till I'm ready?
-------------------- *I graduated from the corner, my respect be my diploma,I went OG for the dollars, Js thought I went to college*
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Mushroom_J
Hard to the Coir !



Registered: 02/17/11
Posts: 774
Loc: East
Last seen: 7 years, 10 months
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Re: Culturing • GRAIN 'PETRI' TEK [Re: GoodyMcLuvin] 1
#21189516 - 01/27/15 11:55 PM (9 years, 3 days ago) |
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My tips...
Dont be afraid of contams.. shit happens. just start over 
Keep things simple
Don't over think
Don't be afraid to ask questions.. even ones you think are dumb.
Also don't expect amazing results your first go... Finding an, or the ideal culture takes time and effort. Regardless of grow style... everyone with a good culture put the effort in to attain it.
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GoodyMcLuvin
Shamanic Level Dimitri Proponent


Registered: 01/12/15
Posts: 74
Loc: Hippie Town, NC
Last seen: 8 years, 4 months
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Mushroom_J]
#21189642 - 01/28/15 12:34 AM (9 years, 3 days ago) |
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Great advice. And I WILL follow it. Thank you.
-------------------- *I graduated from the corner, my respect be my diploma,I went OG for the dollars, Js thought I went to college*
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
There are some who believe that recombination or anamostosis means that some trace genetics from all strains will even be present in all or at least most sectors from cloned fruits, however I do not take this to mean that similar performance can be expected. On the contrary my experiences have tended to indicate the opposite.
We first look for strong healthy growth simply because it is initially the most desirable trait. We worry about the rest later. Chances are that you will not find a non fruiting strain. While poor performers are common I have myself only found truly non fruiting genetics a handful of times.
BOOM.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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