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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Culturing Tech • Agar containers, [Re: twistedty]
#19938607 - 05/04/14 01:33 AM (9 years, 8 months ago) |
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It was spores, made into a spore syringe I do beleive.
Edited by Imperfect Iam (05/04/14 02:20 AM)
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: twistedty]
#19938659 - 05/04/14 01:46 AM (9 years, 8 months ago) |
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And the LC in the pic was made with maybe 2-3cc of the last of the spore syringe, probabably less than 1cc really!
Here's some pics of just now 
Here's to hoping everything stays on track!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Imperfect Iam]
#19978255 - 05/12/14 08:09 PM (9 years, 8 months ago) |
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looks like you have had good success. congratulations.
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20042859 - 05/26/14 01:30 PM (9 years, 8 months ago) |
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yep, be careful if you stack them inside the PC, I tried in spite of the disclaimer. This is what happened: I realized that out of 6 ziploc containers they didn't fit into each other all the same, some were a little looser, so I managed to pair them up, loose one on bottom always. But what I believe caused my doom is that the lid on the top container might have not been unscrewed enough and the pressure inside that container would have made the container expand just enough with the heat pressure to now pretty much air-tight seal the bottom one. I confirm non of the containers were affected by this, which is great, no warps or anything, and they were 3mms away from the PC's edge. Only downside, the grain in the bottom one got really overcooked and took a lot of expansion and started sticking to the top container so it was really hard to separate them, but in the end since I had sealed the top container while manipulating them, and then cleaned the top container with iso before SAB'ing it and re-loosening the lids, I think my 3 top jars are ok. Now I do see that my lids were not un-screwed enough because a day later and condensation is not dissapearing. So my 2 cents, maybe will try stacking again next time but really will unscrew the lids more to avoid pressure build-up (even with GE) inside the containers... If that doesn't work, won't stack them, it's not the end of the world. If ever I succeed at stacking I will update the post. I think you mentioned Violet that we should get the hang of how much to unscrew the lids with experience, I think that's true, I'm gona hope for better next time
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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dr.alkaline



Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20415264 - 08/13/14 09:47 PM (9 years, 5 months ago) |
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How long does it take to get pins from this TEK after bottom watering and cracking the lid to allow gas exchange? I have had a couple of these going grown past consolidation for quite a while, but have yet to see pins. I am using brown rice as a substrate.
Edited by dr.alkaline (08/13/14 09:49 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: dr.alkaline]
#20418635 - 08/14/14 04:27 PM (9 years, 5 months ago) |
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Quote:
dr.alkaline said: How long does it take to get pins from this TEK after bottom watering and cracking the lid to allow gas exchange? I have had a couple of these going grown past consolidation for quite a while, but have yet to see pins. I am using brown rice as a substrate.
try using a time proven, time tested, results proven TEK first. myself and some other people around here are pretty damn proficient at growing some nice ass mushrooms and still had trouble producing any sort of decent results with the V-tek.
My good cultures don't do well with this TEK and then I tried selecting for a culture suited to this TEK

This was the best culture I got to work with this TEK, and it's still pretty lack luster.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: bodhisatta]
#20418641 - 08/14/14 04:29 PM (9 years, 5 months ago) |
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hi bod
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: cronicr]
#20418763 - 08/14/14 05:00 PM (9 years, 5 months ago) |
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I would say mess around with violet's TEKs after you're proficient
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: bodhisatta]
#20418883 - 08/14/14 05:26 PM (9 years, 5 months ago) |
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Quote:
bodhisatta said: I would say mess around with violet's TEKs after you're proficient
QFT. Nice to see ya back bodhi
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Pastywhyte]
#20419234 - 08/14/14 06:37 PM (9 years, 5 months ago) |
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I've played with this tek a bit and for me its more of a novelty but i can see its validity. It doesn't really suit my needs for larger scale growes but i can see it suiting some people.
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: bodhisatta]
#20419310 - 08/14/14 06:48 PM (9 years, 5 months ago) |
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Quote:
MudaFuka said: I've played with this tek a bit
I doubt it, as you seem to be unaware of what thread you're in. This is a culturing tech, not a "grow tek" per se.
Quote:
bodhisatta said: I would say mess around with violet's TEKs after you're proficient
This is a darn good beginner's approach at agar and culturing. It's very similar to the "pasty plates" one, but screw-top containers that encompass every step of the myco process. If you think pasty's approach is good for beginners, you must agree with this one as well... Especially considering that the cloning method is MUCH easier and snappier than other cloning instructions on the forums.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20419323 - 08/14/14 06:50 PM (9 years, 5 months ago) |
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Quote:
Violet said:
Quote:
MudaFuka said: I've played with this tek a bit
I doubt it, as you seem to be unaware of what thread you're in. This is a culturing tech, not a "grow tek" per se.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: dr.alkaline]
#20419326 - 08/14/14 06:51 PM (9 years, 5 months ago) |
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Quote:
dr.alkaline said: How long does it take to get pins from this TEK after bottom watering and cracking the lid to allow gas exchange? I have had a couple of these going grown past consolidation for quite a while, but have yet to see pins. I am using brown rice as a substrate.
Usually by two weeks after fully colonized I saw pins on many or most of the containers. Less if the containers were inoculated from the middle, meaning their colonization time was significantly longer.
Though I don't water until the first pins have been growing a few days at the soonest; often after the first flush altogether.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20419385 - 08/14/14 07:03 PM (9 years, 5 months ago) |
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Shit my bad. I just read somone ask about bottom watering and i thout this was you outher tek. I have used parts of this tek as well though. My only isue with using screw top pp5s for agar is the tall sides make it a bit harder to make transfers but the visibility is a bit better than pasty plates.
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dr.alkaline



Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20419670 - 08/14/14 08:08 PM (9 years, 5 months ago) |
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Quote:
Violet said:
Though I don't water until the first pins have been growing a few days at the soonest; often after the first flush altogether.
I bottomed watered a day after 100% colonization. I guess I misunderstood the directions, maybe this fucked it up 
So just to get this straight.
1. colonize. 2. crack lid on colonized container 3. wait for pins to bottom water
Right?
Edited by dr.alkaline (08/14/14 08:10 PM)
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: dr.alkaline]
#20420157 - 08/14/14 10:03 PM (9 years, 5 months ago) |
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I kindof doubt that messed anything up, but possibly. Keep waiting! That's the majority of growing.
If you're growing to select ideal clones, what the tech is about, don't bottom-water until after you no longer want clones from a container. Otherwise, wait until the pins appear about half-grown, otherwise they won't really need the water. But I typically water to rehydrate After flushes.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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PurePleasure
Untrusted bacteria cultivator.



Registered: 08/29/14
Posts: 5,893
Loc: Lost
Last seen: 11 months, 22 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20574715 - 09/16/14 10:25 AM (9 years, 4 months ago) |
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-------------------- If you aren't happy with what you have now, how could you be any happier with more? (Get your SGFC high.)

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Fivel
Crazy Smurf



Registered: 07/09/14
Posts: 521
Loc: Midwest
Last seen: 1 year, 10 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: PurePleasure]
#20590420 - 09/19/14 09:14 PM (9 years, 4 months ago) |
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This is fucking brilliant Violet.. I have read this a couple times but it seriously just clicked.. amazing.
-------------------- "The only way to deal with an unfree world is to become so absolutely free that your very EXISTENCE is an act of rebellion." - Albert Camus Have Orissa Prints for trade! In search of PF albino, PF redspore, PF classic "... the associations between sadism and GAIT (Global Assessment of Internet Trolling) scores were so strong that it might be said that online trolls are prototypical everyday sadists."
         
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Myceliumcake
addicted to weed
Registered: 07/17/00
Posts: 405
Loc: Flint, Michigan
Last seen: 1 year, 8 months
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Fivel]
#20600705 - 09/22/14 11:14 AM (9 years, 4 months ago) |
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
#20626128 - 09/27/14 02:22 PM (9 years, 4 months ago) |
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Can someone share with us the dimensions of the different twist n loc containers? They come in various shapes and sizes and the specifics are amazingly hard to find. I can't get these locally, they don't carry them.
Even ziploc.com doesn't have the information and their customer service center flatly refused to provide the information. I don't know weather to laugh or write them a nasty letter, is it a secret?
The medium round twist n loc is 6 inches high and 4.5 inches wide. Shhhh don't tell anyone!
I'm also looking for the dimensions of the ziploc "smart snap", which has the snap on style lids (I assume these can survive a pc run right?).
Is there any other brand that can stand up to a pressure cooking that you guys know of?
Edited by invitro (09/27/14 02:34 PM)
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