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Offlinerobbinbanks
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Re: Culturing • GRAIN 'PETRI' TEK [Re: robbinbanks]
    #19475219 - 01/26/14 04:35 AM (10 years, 5 days ago)



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Invisibleblindingleaf
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Re: Culturing • GRAIN 'PETRI' TEK [Re: robbinbanks]
    #19475453 - 01/26/14 06:26 AM (10 years, 5 days ago)

disclaimer: violet will have way better advice than me, but i will chime in.  take her advice over mine
yea they look a bit dry cause that white spot at the end of each grain.  u can try tho if u have a wedge.  but for ms, i'd say try again.
i have made v teks too wet and growth was slow, but it did complete.  as far as casing ur v tek that may be on the dry side, not sure, u would have to take a picture.  if i had to do the uncased v tek again, i would consolidate for longer, that seemed to be my only mistake.  the cased turned out way better.  what i have been doing for the uncased that were consolidated, but not long enough, is bottom watering during first flush, and it seems to be making a difference.
BR is hard to get right, i'd have to look at my note book to see how i did it right.  out of 4 tries, it seems that only the last time worked to "perfection".  the main thing, IMO, is making sure to account for the additional expansion/cooking of each grain as it goes thru the PC cycle, which makes it difficult.  you don't want it "just right" before PCing, you want it a LITTLE less.  otherwise it gets over saturated and can stall growth, and u lose the nutrients that make the v tek what it is.


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinerobbinbanks
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Re: Culturing • GRAIN 'PETRI' TEK [Re: blindingleaf]
    #19475473 - 01/26/14 06:34 AM (10 years, 5 days ago)

well thats good cuz i havent pced yet and they are going through the pre ferment stage after I did a second heat treatment.I would love to here how you got your grains perfect because i was a little confused with violets grain prep. I couldnt figure out if you could use the microwave second heat treatment when flash hydrating BR. Can you flash hydrate BR? the rise easily splits with finger nail and is not starchy:confused:


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Invisibleblindingleaf
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Re: Culturing • GRAIN 'PETRI' TEK [Re: robbinbanks]
    #19475486 - 01/26/14 06:40 AM (10 years, 5 days ago)

disclaimer: violet will have way better advice than me, but i will chime in.  take her advice over mine
it was a lot of hit and miss with mine.  id didn't do the flash hydration (i think its the second one)  cause a lot of BR kernels split.  i soaked for 12 hours or so, and boiled for maybe 5 minutes, i'd have to look back and see.  what u describe is perfect actually with the easy splitting.  put one container thru a pc cycle and see how they come out.  remember its only a 40 minutes pc cycle, unless ur doing a lot.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinerobbinbanks
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Re: Culturing • GRAIN 'PETRI' TEK [Re: blindingleaf]
    #19475490 - 01/26/14 06:41 AM (10 years, 5 days ago)

will do, thanks my friend:cool:


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Offlinexerolad
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Re: Culturing • GRAIN 'PETRI' TEK [Re: robbinbanks]
    #19524632 - 02/05/14 10:33 AM (9 years, 11 months ago)

I'm bookmarking this thread for sure. Tons of useful info here. Violet, you are an innovator.


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Offlinesupernewfie
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: barong]
    #19530947 - 02/06/14 02:39 PM (9 years, 11 months ago)

Quote:

barong said:
Quote:

Violet said:that grainwater contains the same spectrum of materials that the grains




But it does not contain the same spectrum of 'materials' that malt extract does. I'm not suggesting it will or won't work, but it's not even close to being malt extract. How that impact mycelium growth remains to be seen . I'm sure it would work, hell dogfood works.

And for all the time and mess, plus timing issues, or storage requirements, for your grainwater against having malt extract quickly and readily on hand, there's really no point, apart from it being another 'novel' idea of yours. There's plenty of people wishing to reinvent the wheel, and good luck to you, if it works.

Cost of 1lb malt extract = $4.50
Cost of 20g malt extract = 20 cents
Cost of malt extract per 20mL solution for plate = 1/2 a cent !

Cost saving by using grainwater = an almost irrelevant arguement





violet knows whats up. and as you can see it is working.... some fucking people......


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Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
    #19538284 - 02/08/14 06:43 AM (9 years, 11 months ago)

Quote:

Violet said:
"An almost irrelevant argument."  Yet you're the one making it here, yes?

Sure malt is cheap.  However its cheapness is no concern of mine because spending even a dime on it is a waste since I don't need it, I already buy grains.

Thanks for your demeaning posts.




less is more .. :-)

yes malt is cheap, but doing stuff yourself is more rewarding often and saving the environment is too, if it only means maybe 5mins of work

nice tek, gotta try the agar stuff, with petris
oatmeal agar is a hassle to make each time, even though it seems perfect for agar work
if only plastic petris were easily reuseable(I reuse mine)... that was the biggest benefit when I used pre-poured jars
contam hasn't been an issue, normal plastic film works great for petris here
can even have them laying around in the house, no contam, but mycellium grows fast


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Offlineoxana
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: lessismore]
    #19545499 - 02/09/14 08:16 PM (9 years, 11 months ago)

so im planing to isolate some new strains from spores
how would you recomend starting from spore syringes? i have to use syringes its for penis envy and albinos

could i drop a single drop in the middle of a brown rice puck?
what about on agar? how did you inoculate the container in the picture? was this from a syringe and then spread around?




PS how much do you dilute your rice water agar before adding the agar.


Edited by oxana (02/09/14 08:31 PM)


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InvisibleViolet
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: oxana]
    #19545565 - 02/09/14 08:35 PM (9 years, 11 months ago)

From spore syringes, I recommend the "grain petris", exactly as you asked. 1-3 drops should be good.

The dish in the photo was spore print inoc, scattered spores off one of my sterile-taken prints with scalpel.

I don't dilute the ricewater at all, I only make sure it's very well settled and use the "clear" liquid that can be poured off the top.


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Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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Offlineoxana
i am the fun guy

Registered: 12/14/08
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
    #19546128 - 02/09/14 11:33 PM (9 years, 11 months ago)

excellent that answers all my questions. thanks so much


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InvisibleWillSolvem
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: oxana]
    #19578556 - 02/17/14 07:37 AM (9 years, 11 months ago)

Haven't had much to update. I put both isolates to a grain master then G2G to ten yesterday. I have definitely noticed an increase in colonizing speed/rate/aggressiveness in the side by side grow of "Santa" and "Fatboy" (see previous posts) the latter being the one standing out. I have one more G2G before I have enough grain to spawn so I'll update in about a month.

Hey good to see everyone :thumbup:


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AMU Q&A Thread because questions deserve answers.


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InvisibleViolet
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: WillSolvem]
    #19588956 - 02/19/14 08:15 AM (9 years, 11 months ago)

3 months later and my elimination-round isolate tests are still putting up fruits.


NO contams, out of ~25 containers.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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Invisibleblindingleaf
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spores! [Re: Violet]
    #19588994 - 02/19/14 08:30 AM (9 years, 11 months ago)

doing another v tek round.  this time i will consolidate the rye grains.  mostly doing brf recipe with some cereal supplement (bob red mill 10 grain hot cereal i think its called) but following tek otherwise.  only 3 containers total.  only pint size also, so will have to figure that one out b/c i don't have a SGFC big enough or an unused mono tub tote to fit them (3 more an uninnoculated as of now).  will be casing ALL of them this time also, as i had better performance with cased





still got side pins.  maybe casing was too thick? (equal to substrate)



uncased did not perform well.  i did not let it consolidate long enough and probably screwed up the loose lid thing, too loose made it dry up.





also spawning enoki to sawdust in two of them, but don't know if tek applies to that or not.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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InvisibleImperfect Iam
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Violet]
    #19592332 - 02/19/14 10:08 PM (9 years, 11 months ago)

I am glad I saved some of my grain water last night! I also am happy I ran across this thread!  EXCELLENT EXPERIMENTATION Violet, I do believe this is the first time I ran across you too, I do beleive, sure glad I did! Keep up the good  work!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Imperfect Iam]
    #19592591 - 02/19/14 11:12 PM (9 years, 11 months ago)

tested the other tek(grass seed tek) 2 times, unfortunately I managed to screw up both times

too impatient lol, it seems very important that they receive fully boiling water
or they will fail, 4 out of 4 failed jars this time, same last time :frown:

maybe my electric kettle doesn't work too good(it has a small leak in the bottom I just found out), should probably use water from the stove next time

the grass seeds definitely didn't seem to expand much at all, bad sign?
but I managed to screw up WBS 2 times too at first(boiling them, not drying enough), so same with this tek

will be trying again, with water from the stove
practice makes perfect, can't possibly do it wrong in 3rd try ;P
and agar cultures are clean(they were for this test too), so one less possibility for mistakes

did save all the grain water though, can always be used
although I really like oatmeal, it is perfect, just made 2litre of that


Edited by lessismore (02/19/14 11:18 PM)


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InvisibleImperfect Iam
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: lessismore]
    #19592757 - 02/19/14 11:59 PM (9 years, 11 months ago)

I didn't get to read through this whole thing, but I am going to because I think may try to make some tonight, except all the stores are closed and I am out of Petris, gonna scrounge around and see what I can't come up with!

And congratulations on not throwing in the towel, I respect that, Hell someone even told me to quit or give up, But then retracted his statement and is not a bad guy!

Sometimes some people, me included, write things w/o thinking about it first!


--------------------
All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Offlinenorthview
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: Imperfect Iam]
    #19670717 - 03/09/14 06:50 AM (9 years, 10 months ago)

I have one question pertaining to the lids on these PP5 containers, I am not seeing any ports, do you just PC these with the lids cracked slightly? I assume with no SFD there is no need for foil over lids?
Great thread, great info, only question I developed.


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Invisibleblindingleaf
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: northview]
    #19670734 - 03/09/14 07:00 AM (9 years, 10 months ago)

correct.  for v tek there is no lid modification necessary.  only slightly cracked lids, like with a leftover microwave dinner, maybe even less "cracked".  1/8-1/4 turn pre PC, then tighten quickly as u take them out of PC.
  she can guide u better if u r attempting the TEK start to finish, but i still use the pp5's for small grain amts, or saw dust amounts.  i have not used foil yet. unless ur PC is heavily loaded with PP5's, and the stove is GAS, u will be fine.  if u have a GAS stove, and the PC is heavily loaded with pp5's, just make sure whatever portion of the plastic that is touching the walls is covered in foil, or line the walls of the PC with foil before starting the cycle, EM666 style.
if ur stove does not use an open flame as a heat source, i think u are fine w/o foil.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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Offlinenorthview
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Re: Culturing Tech • Agar containers, "Grain Petri" Tek, Invitro growing & Sterile Spore Printing! [Re: blindingleaf]
    #19670779 - 03/09/14 07:24 AM (9 years, 10 months ago)

Thank you very much. Is it best to let the PC sit over night to cool when sterilizing these? (Like one would with RR's rye tek?)




Edit:extraneous info.


Edited by northview (03/09/14 06:24 PM)


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