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MaJiK_420
...lost



Registered: 06/30/08
Posts: 447
Last seen: 6 years, 1 day
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Re: Culturing • GRAIN 'PETRI' TEK [Re: bluecap]
#19337869 - 12/28/13 05:20 PM (10 years, 1 month ago) |
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I apologize if it has been asked/answered already, I only read first and last pages.
But I am really trying to get into the Agar thing, I hated that plastic petris are one time use and I really like using the PP5 containers.
However one issue I keep having is wicked condensation. I'll even let my agar cool a little so its not steaming before I lid it and cook it, but I think steam is getting in when I cook em. So much that by the time I pull em out the walls are covered in condensation droplets on the inside. Then it seems like the water droplets fall and make the agar all splotchy and it just looks bad. I can't tell if it is affecting growth, but I almost feel like the water splotches breed some kind of bacteria. Can post pics if it helps.
How do I get rid of the condensation problem>? I wanted to try stacking them, 16 in at once like you show, but they fit so well together I feel like they might be creating an air tight seal that might lead to warping or something.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Culturing • GRAIN 'PETRI' TEK [Re: MaJiK_420]
#19337917 - 12/28/13 05:28 PM (10 years, 1 month ago) |
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i always pour my agar into my containers then wait for it to get to room temp before i put the lid on and pc, i then leave mine in the pc over night. remember condesaion occurs with temp diffrence but even if you do get it it's not the end of the world and should go away over the course of a few days IME...hi violet
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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J. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
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Re: Culturing • GRAIN 'PETRI' TEK [Re: MaJiK_420]
#19339554 - 12/28/13 11:35 PM (10 years, 1 month ago) |
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same boat, really. i have two PP5 agar cultures going. as you said wicked condensation inside. i shelve them upside down and they seem to be doing just fine. they were cooled completely in the PC before i opened it up and tightened the lids.
my idea for dealing with this next time is to prepare the jars in advance, loosen the lids to almost completely open to let the vapor out in a cool place (basement) then close them and bring them upstairs. then they're ready for action. in fact... i'm gonna do that right now. we'll see how it goes.
i did the stacking thing for some rice plates, and it worked fine.
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the j stands for jesus.2020 new years grow along
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Violet



Registered: 12/06/11
Posts: 4,205
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If you pour a glass of ice water don't consider it a problem when physics guarantees condensation on the glass. Condensation happens. It's a major thing with pre-pour. You may learn some tricks to minimize it, but the real trick to learn is avoiding problems with it. Good clean technique will ensure your dishes don't become rife with bacteria.
Personally I shake out the moisture from the containers after I pull them from the cooker. There's a bit too much for the agar to absorb it all, but after getting rid of loose liquid there's just enough. The surface of my agar stays dry for the whole run as long as I dump out moisture before inoculating.
This is not a problem for me in sterile laminar airflow, but be careful about it when using SAB.
Hey Chron!
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Violet]
#19351310 - 12/31/13 02:13 PM (10 years, 30 days ago) |
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cased with 50/50+hydrated lime in FC now. some fruits grew but camera battery dead at the time. small fruits, so nothing special missed.

also got these

larger containers. def gonna case. majority were agar wedges, 3 of them were honey LC started with an agar wedge. like this one

also did some with BRF mix instead of just WGBR. violet, do u wait for knots to form before untwisting a bit if u grow them uncased? for casing, what is best time to case..? right at 100% or a few days later? if casing applied, do u put back in incubation with loose lid or just put straight into FC with such a this casing layer?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Culturing • GRAIN 'PETRI' TEK [Re: blindingleaf]
#19351561 - 12/31/13 03:26 PM (10 years, 30 days ago) |
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Nice bro I used to buy those same plastic containers now I use empty italian Gelato containers. Make supa-cakes
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Doc Seta]
#19368317 - 01/04/14 01:38 PM (10 years, 26 days ago) |
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Hey Violet, again nice write up.
Just a quick question, I ran out of petri dishes and was thinking of doing this with pins. Have you noticed any differences with using pins to inoculate these rice dishes? I have a tub with odd genetics and was planning on transferring some of those pins onto the rice pucks. Thanks
Also just a side note, have you ever left a specimen in the fridge until it turned blue and then placed it on a rice puck?
Thanks for the help and I am a big fan of your work. PEACE
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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hey guys...thought id share my progress with this tek and maybe get some pointers


so those are the mini mini pp.'s in the first pic, in a mono-ish hybrid thing. bottom is 2" perlite. the containers are actually submerged in the perlite, as u can see here:

this was my first attempt at casing. 50 peat/50 verm + though i may have added to much hydrated lime (i did 1 tsp per cup of peat, a standard measure by RR) the containers were consolidated for 1 week after 100%. I'm still getting side pins despite being submerged in perlite (i was hoping to keep humidity high as well as block out light)


the second pic i posted above looks like it will do better, i will not b using an FC for this one, just loose lid. growth looks good into casing. this casing is a bit different 50% peat, 40% verm, 10% coir 1 tsp hydrated lime per 2 cups peat.
im not sure why its (the mini containers) not fruiting better, all i can think is the lime was too much, but this was/coir/verm did not seem to mind it (this is same casing, from same pasteurized qt jar so 1tsp hydrated lime per cup of peat) this was also half harvested so i could reach hands in to pull it out of container:

im also noticing that the mushrooms have this skin to it and when picked, that skin is kind of attached to the sub, sometimes a little vein-y other times like a matted skin. I'm guessing its the myc trying to protect itself against the lime, but thats all guesswork. i can;t seem to find any info/pics about over limed casings, other than posts stating that the myc won't grow thru it.
im also wondering, with the side pins plus a casing, should i try to lift it out of container, pic side pins, then put back and bottom water? i did not see bottom watering pics on this tek unless there was NO casing. is bottom watering not recommended with a casing?
im also wondering if as time goes by and the hydrated lime loses its effect, if it will pin better as the casing gets more acidic
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: sharing progress and troubleshooting [Re: blindingleaf]
#19369048 - 01/04/14 04:22 PM (10 years, 26 days ago) |
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I bet that the side pins will occur no matter what kind of casing or what ph its at. Cubensis prefers to pin from the sides and if the opportunity to pin from the side presents itself it will pour all its energy into pinning from the side regardless of what kind of conditions are available on top.
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Violet]
#19369307 - 01/04/14 05:15 PM (10 years, 26 days ago) |
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Violet, I had a thought about side pinning. If you recall the straw log video from RogerRabbit he says that when the log is fully colonized, you should slit the plastic lengthwise along the log and fan it out a few times a day and close it back up. This will cause lots of pinning because you get 100% humidity and FAE.
So with the v-tek could you cut out a circle of thin plastic (like 2 mil give or take) and lay it over the top of the "straight grain cake" in order to stimulate pinning on the top? You might have to remove said cover a few times a day. I don't have the time to experiment on this concept but maybe someone else could.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Culturing • GRAIN 'PETRI' TEK [Re: invitro]
#19369330 - 01/04/14 05:19 PM (10 years, 26 days ago) |
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search the bubble wrap tek
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Culturing • GRAIN 'PETRI' TEK [Re: cronicr]
#19369785 - 01/04/14 06:55 PM (10 years, 26 days ago) |
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I have a great solution for side pinning varieties, obtain some fluorescent light grid covers, cut to sizeand place over substrate directly, over casing, or place over substrate and fill grid with casing material. It gives the mycelium the myco-environment it craves across the surface of your substrate. They are reusable but pricy. I'll dig up some photos of them in use but you get the idea
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AMU Q&A Thread because questions deserve answers.
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Culturing • GRAIN 'PETRI' TEK [Re: WillSolvem]
#19370330 - 01/04/14 09:16 PM (10 years, 26 days ago) |
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I'm not sure how the grid helps, how is this different from just casing?
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mushrume man
Sadis Factory



Registered: 11/18/08
Posts: 375
Loc: PNW
Last seen: 2 years, 17 days
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Re: Culturing • GRAIN 'PETRI' TEK [Re: WillSolvem]
#19371119 - 01/05/14 01:36 AM (10 years, 26 days ago) |
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Quote:
WillSolvem said: I have a great solution for side pinning varieties, obtain some fluorescent light grid covers, cut to sizeand place over substrate directly, over casing, or place over substrate and fill grid with casing material. It gives the mycelium the myco-environment it craves across the surface of your substrate. They are reusable but pricy. I'll dig up some photos of them in use but you get the idea

Weird I had the same thought the other day
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WillSolvem
Odd-Hand




Registered: 04/24/12
Posts: 1,519
Loc: Chapter 26
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Re: Culturing • GRAIN 'PETRI' TEK [Re: invitro]
#19371452 - 01/05/14 05:37 AM (10 years, 26 days ago) |
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Quote:
invitro said: I'm not sure how the grid helps, how is this different from just casing?
It gives a vertical surface across a horizontal plane
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AMU Q&A Thread because questions deserve answers.
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oxana
i am the fun guy

Registered: 12/14/08
Posts: 258
Last seen: 2 years, 13 days
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Re: Culturing • GRAIN 'PETRI' TEK [Re: WillSolvem]
#19383647 - 01/07/14 04:49 PM (10 years, 23 days ago) |
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hey got a question violet.
how do you apply your spores to the surface of you agar? do you streak it with an inoculation loop from a spore print or do you have a different method?
ive made about 40 "agar containers" and i like working with them more than regular petri dishes. i just wish they made the top clear rather than blue.
i think im going to do a grow journal tonight using your V tek. you should pop in and see if im meeting your specifications for you process, ill probably have some questions also.
thanks
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Culturing • GRAIN 'PETRI' TEK [Re: WillSolvem]
#19386070 - 01/08/14 12:29 AM (10 years, 23 days ago) |
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Quote:
WillSolvem said: I have a great solution for side pinning varieties, obtain some fluorescent light grid covers, cut to sizeand place over substrate directly, over casing, or place over substrate and fill grid with casing material. It gives the mycelium the myco-environment it craves across the surface of your substrate. They are reusable but pricy. I'll dig up some photos of them in use but you get the idea

UGH Can't take the side pinning, after 2-3 flushes I had to empty some casings and cause fruits were basically growing invitro along the sides an beneath. Its most likely from the shrinkage after each flush but I always fill the sides back in. Any info much appreciated
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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Doc Seta]
#19386377 - 01/08/14 02:43 AM (10 years, 23 days ago) |
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Quote:
Doc Seta said:
Quote:
WillSolvem said: I have a great solution for side pinning varieties, obtain some fluorescent light grid covers, cut to sizeand place over substrate directly, over casing, or place over substrate and fill grid with casing material. It gives the mycelium the myco-environment it craves across the surface of your substrate. They are reusable but pricy. I'll dig up some photos of them in use but you get the idea

UGH Can't take the side pinning, after 2-3 flushes I had to empty some casings and cause fruits were basically growing invitro along the sides an beneath. Its most likely from the shrinkage after each flush but I always fill the sides back in. Any info much appreciated
Try using a liner.
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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Doc Seta
Sinister Das Trip Sober



Registered: 12/18/13
Posts: 550
Loc: Tunafish, Ocean
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Re: Culturing • GRAIN 'PETRI' TEK [Re: mushmagic]
#19386642 - 01/08/14 06:27 AM (10 years, 23 days ago) |
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Tried all kinds of stuff, btw nice grow u got there Im guessing EQs?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Culturing • GRAIN 'PETRI' TEK [Re: Doc Seta]
#19387116 - 01/08/14 09:47 AM (10 years, 22 days ago) |
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The gap between the tub and your substrate makes a good microclimate for side pins, Get a good liner(see franks threads) and the liner will stick to your substrate leaving pins feeling like they want to form on the top
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