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OfflineMosey3012
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Registered: 12/12/12
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Best Agar Reciepe for Stone Producers?
    #19032837 - 10/25/13 07:48 PM (11 years, 2 months ago)

I've got prints for both Psilocybe galindoi and Psilocybe mexicana which so far I've had no luck getting to grow out with the agar recipe I'm using now (Or any spores that I've ever tried as far as I recall). At the moment I've been working with a tek Doc_T posted:

http://www.shroomery.org/forums/showflat.php/Number/16590981#16590981

which simply just uses 6g of malt and 5g of agar to 340mL water, making about ~15 jars. I haven't bothered to get any petri dishes since well I've been having perfectly fine success using jars and the above mixture working with clones and pieces of colonized grain. I'll eventually move to petri's when I feel like I'm sufficiently skilled at making transfers and getting a couple isolates actually grown out so lets not bother talking about my choice of container please.

Using the above recipe doesn't ever seem to get me much of that long ropey rizo growth I see a lot of other people post pics of either... Perhaps in very tiny sectors which I understand is something I'd have to isolate out thru transfers but for the most part it just seems to be thicker fluffy growth across the plate... Never any even smaller areas where its easily noticeable.  Don't know if the particular recipe has anything to do with that or if as I've been making my transfers all year I've just been isolating tomentose instead of rizo lol. Thought id mention this as well to see if anyone has thoughts on this.

To keep it short and sweet. From your personal experience what has been the best agar recipe for you to grow out spores on? In addition, would you use that same recipe for stone producers or is there another you'd personally suggest?

I appreciate the feed back!

Mosey

Edited by Mosey3012 (10/25/13 07:56 PM)

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InvisibleViolet
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Registered: 12/06/11
Posts: 4,205
Re: Best Agar Reciepe for Stone Producers? [Re: Mosey3012]
    #19035238 - 10/26/13 11:33 AM (11 years, 2 months ago)

We grow all of these species on grain - I grow all of them on grainwater agar left over from my grain preparations.


Not a stone-producer, but wonderful spore germ nonetheless.

There's no reason to suppose that they'd germinate less wonderfully than they do on grains, it's the same stuff.

In fact IMO this may be more optimal than using a single purified ingredient (malt) extracted from grains anyway.
I think, why would people say 2 tablespoons of BRF per 500mL water is too nutritious, when supposedly the uber-sweet mixture of 10g malt sugar grain extract is just right?


Since you asked about this as well, I also get great rhizo from grainwater agar.


--------------------
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PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,097
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Re: Best Agar Reciepe for Stone Producers? [Re: Violet]
    #19035344 - 10/26/13 11:57 AM (11 years, 2 months ago)

I just use a regular MEA. I weigh it out and do the math to get 2% malt content or so, then use 20g of agar agar per L. You could also try PDA, or make a sugar-based agar such as Karo, honey or turbinado sugar.

Anything will work.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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