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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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InvisibleEastBayRay
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Registered: 06/06/13
Posts: 746
MEA Only Thing Needed for Culture Slants?
    #19032415 - 10/25/13 06:21 PM (10 years, 3 months ago)

Besides a poopsicle stick, toothpick, or wooden dowel and water, is MEA the only thing needed for a culture using polyfilm and vacutainer lids and tubes?

Can I just get away with using the following for long-term culture storage?:

MEA http://fungi.com/product-detail/product/antibiotic-malt-extract-agar-12-pound.html

Vacutainer Tube

Red Vacutainer Lid

Parafilm

Wooden Dowel

H2O


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InvisibleEastBayRay
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Registered: 06/06/13
Posts: 746
Re: MEA Only Thing Needed for Culture Slants? [Re: EastBayRay]
    #19051560 - 10/29/13 11:29 AM (10 years, 3 months ago)

Anyone?

With so many recipes out there it's made me confused.  Isn't plain old antibiotic MEA good enough on it's own?


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: MEA Only Thing Needed for Culture Slants? [Re: EastBayRay]
    #19051605 - 10/29/13 11:39 AM (10 years, 3 months ago)



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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: MEA Only Thing Needed for Culture Slants? [Re: bodhisatta]
    #19051637 - 10/29/13 11:45 AM (10 years, 3 months ago)

Just use regular MEA. Antibacterial agar should only be used if you have a bacteria problem.


--------------------
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Pat The Bunny said:
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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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InvisibleEastBayRay
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Registered: 06/06/13
Posts: 746
Re: MEA Only Thing Needed for Culture Slants? [Re: 36fuckin5]
    #19051906 - 10/29/13 01:05 PM (10 years, 3 months ago)

Thanks! 

After inoculation how long should one wait before the slants are put into refrigeration?  Should they be put in the fridge immediately after inoculation, after some growth, or after fully colonized?


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InvisibleEastBayRay
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Registered: 06/06/13
Posts: 746
Re: MEA Only Thing Needed for Culture Slants? [Re: EastBayRay]
    #19051990 - 10/29/13 01:30 PM (10 years, 3 months ago)

I found my answer.

http://www.shroomery.org/forums/showflat.php/Number/13696637#13696637

"I let the mycelium jump off the transferred piece into the fresh agar in the slant before refrigerating.  No more than 5 or 6 days.  The slant doesn't need to fully colonize, but in a few months when you pull it out of the refrigerator, it will be full of mycelium."
RR


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