Home | Community | Message Board

Cannabis Seeds UK
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineJahLambsbread
Stranger

Registered: 08/08/10
Posts: 684
Last seen: 6 years, 4 months
Remove tissue from agar?
    #19031270 - 10/25/13 01:59 PM (10 years, 3 months ago)

I have some stigma tissue(from the inside of the stem) on a petri dish. two pieces of tissue and one of them is about a centimeter long.


There is a lot of mycelium growth but I see some yellow spots. Is this contamination?

Should I remove the tissue from the agar to reduce contamination chances?


The tissues were taken from dry mushrooms so I soaked them in distilled water so they were pretty wet when put on the agar.


Extras: Filter Print Post Top
Invisible8ow8
Soylent McGriddle
Male


Registered: 05/22/13
Posts: 196
Loc: Canada
Re: Remove tissue from agar? [Re: JahLambsbread]
    #19031349 - 10/25/13 02:19 PM (10 years, 3 months ago)

Don't remove the contaminants from the petri dish! You'll likely just spread them around.

Take a clean wedge of uncontaminated mycelium and transfer it to another plate. Hopefully the contams won't come along. If they do, let the culture grow a bit, and then take another sample from a clean area on the second plate and transfer to a third. Continue until your culture is clean. All but the fastest contaminants can be cleaned this way.

This is simple agar transfer - an absolutely necessary technique for getting rid of contaminants. There are tons of teks, including RRs Let's grow mushrooms videos (which I highly recommend).


--------------------
“There you go. Giving a fuck when it ain’t your turn to give a fuck.”

My Trade list


Extras: Filter Print Post Top
OfflineJahLambsbread
Stranger

Registered: 08/08/10
Posts: 684
Last seen: 6 years, 4 months
Re: Remove tissue from agar? [Re: 8ow8]
    #19031364 - 10/25/13 02:23 PM (10 years, 3 months ago)

ok but should i remove the tissue?

That is my original question that is in the title and needs to be answered asap.


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,847
Loc: In the Gills
Last seen: 1 hour, 13 minutes
Re: Remove tissue from agar? [Re: JahLambsbread]
    #19031397 - 10/25/13 02:29 PM (10 years, 3 months ago)

no don't remove the original tissue. Just cut a piece of clean myce from a leading growing edge and transfer that cut to another petri


--------------------

Links For Beginners And Beyond:mspoil:


Extras: Filter Print Post Top
Invisiblepoopy mcpooperson
Pogonophobia
 User Gallery


Registered: 10/17/13
Posts: 124
Re: Remove tissue from agar? [Re: rumfor69]
    #19031651 - 10/25/13 03:24 PM (10 years, 3 months ago)

rumfor69 is completely correct. dont remove the tissue.

buy this entire video series.

http://www.mushroomvideos.com/Strain-Isolation

this preview should give you an idea of what you need to do.


--------------------
love and laughter  :laugh:


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,847
Loc: In the Gills
Last seen: 1 hour, 13 minutes
Re: Remove tissue from agar? [Re: poopy mcpooperson]
    #19031759 - 10/25/13 03:44 PM (10 years, 3 months ago)

Quote:

poopy mcpooperson said:
rumfor69 is completely correct. dont remove the tissue.

buy this entire video series.

http://www.mushroomvideos.com/Strain-Isolation

this preview should give you an idea of what you need to do.




One of my favorite videos, i lost my copy in a hard drive crash though :frown:


--------------------

Links For Beginners And Beyond:mspoil:


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 5 days
Trusted Cultivator
OG Cultivator
Re: Remove tissue from agar? [Re: rumfor69]
    #19033246 - 10/25/13 09:23 PM (10 years, 3 months ago)

Send the confirmation email and I'll reinitialize the download.

To the original poster, if all you have is dry tissue you need to get some spores off the gills to germinate.  Getting dry mycelium to grow again can be done but it takes a lot more work than that.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Offlineskippydude
Myco-curious
Male User Gallery


Registered: 09/08/12
Posts: 1,827
Loc: "Alice's Wonderland"
Last seen: 9 years, 2 months
Re: Remove tissue from agar? [Re: RogerRabbit]
    #19033315 - 10/25/13 09:39 PM (10 years, 3 months ago)

The yellow spots are bacteria, which is much easier to isolate from than mold :thumbup::goodluck:
http://www.shroomery.org/forums/showflat.php/Number/17451779


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,847
Loc: In the Gills
Last seen: 1 hour, 13 minutes
Re: Remove tissue from agar? [Re: RogerRabbit]
    #19035494 - 10/26/13 12:34 PM (10 years, 3 months ago)

Quote:

RogerRabbit said:
Send the confirmation email and I'll reinitialize the download.

To the original poster, if all you have is dry tissue you need to get some spores off the gills to germinate.  Getting dry mycelium to grow again can be done but it takes a lot more work than that.
RR




Ya i deleted the email cause im genius like that lol its ok i will just buy it again. Its not like you havent done enough for me, its very cost effective lol 


OP: I missed the part about the original sample being dry flesh (hate it when i do that) RR is right this could be very hard. I think you could easily confuse contam myce with normal myce crossed with bacteria smudges lol your best bet is to use flesh from the gills like he said that will have spores on them. It wont be a clone really but it will live.


--------------------

Links For Beginners And Beyond:mspoil:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* Agar culture highly contaminated CultyVader 3,101 10 03/25/03 05:51 PM
by CultyVader
* Re: mold in agar plate Anonymous 12,274 3 12/04/99 12:35 AM
by Anonymous
* agar transfers *DELETED* thisone 1,247 3 11/20/03 08:58 PM
by Mycomancer
* Inoculating with mushed up shroom tissue..?
( 1 2 all )
Openminded 7,282 34 06/26/17 09:37 AM
by bodhisatta
* Re: Agar w/ h202, any info?? 3Mshroom 2,155 8 05/08/00 04:25 AM
by mycofile
* Agar to Rice flour substrates foxxybrown 2,142 3 09/01/03 05:00 AM
by eggy
* golden teacher agar asking fugu 3,068 6 01/31/03 12:59 PM
by debianlinux
* Contamination problems need advice(help) Ganoderma 2,316 9 09/11/03 12:35 AM
by Ganoderma

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
971 topic views. 20 members, 144 guests and 20 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.01 seconds on 14 queries.