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JahLambsbread
Stranger

Registered: 08/08/10
Posts: 684
Last seen: 6 years, 4 months
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Remove tissue from agar?
#19031270 - 10/25/13 01:59 PM (10 years, 3 months ago) |
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I have some stigma tissue(from the inside of the stem) on a petri dish. two pieces of tissue and one of them is about a centimeter long.
There is a lot of mycelium growth but I see some yellow spots. Is this contamination?
Should I remove the tissue from the agar to reduce contamination chances?
The tissues were taken from dry mushrooms so I soaked them in distilled water so they were pretty wet when put on the agar.
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8ow8
Soylent McGriddle



Registered: 05/22/13
Posts: 196
Loc: Canada
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Don't remove the contaminants from the petri dish! You'll likely just spread them around.
Take a clean wedge of uncontaminated mycelium and transfer it to another plate. Hopefully the contams won't come along. If they do, let the culture grow a bit, and then take another sample from a clean area on the second plate and transfer to a third. Continue until your culture is clean. All but the fastest contaminants can be cleaned this way.
This is simple agar transfer - an absolutely necessary technique for getting rid of contaminants. There are tons of teks, including RRs Let's grow mushrooms videos (which I highly recommend).
-------------------- “There you go. Giving a fuck when it ain’t your turn to give a fuck.” My Trade list
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JahLambsbread
Stranger

Registered: 08/08/10
Posts: 684
Last seen: 6 years, 4 months
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Re: Remove tissue from agar? [Re: 8ow8]
#19031364 - 10/25/13 02:23 PM (10 years, 3 months ago) |
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ok but should i remove the tissue?
That is my original question that is in the title and needs to be answered asap.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,847
Loc: In the Gills
Last seen: 1 hour, 13 minutes
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no don't remove the original tissue. Just cut a piece of clean myce from a leading growing edge and transfer that cut to another petri
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poopy mcpooperson
Pogonophobia



Registered: 10/17/13
Posts: 124
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Re: Remove tissue from agar? [Re: rumfor69]
#19031651 - 10/25/13 03:24 PM (10 years, 3 months ago) |
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rumfor69 is completely correct. dont remove the tissue.
buy this entire video series.
http://www.mushroomvideos.com/Strain-Isolation
this preview should give you an idea of what you need to do.
-------------------- love and laughter
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,847
Loc: In the Gills
Last seen: 1 hour, 13 minutes
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Quote:
poopy mcpooperson said: rumfor69 is completely correct. dont remove the tissue.
buy this entire video series.
http://www.mushroomvideos.com/Strain-Isolation
this preview should give you an idea of what you need to do.
One of my favorite videos, i lost my copy in a hard drive crash though
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 5 days
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Re: Remove tissue from agar? [Re: rumfor69]
#19033246 - 10/25/13 09:23 PM (10 years, 3 months ago) |
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Send the confirmation email and I'll reinitialize the download.
To the original poster, if all you have is dry tissue you need to get some spores off the gills to germinate. Getting dry mycelium to grow again can be done but it takes a lot more work than that. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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skippydude
Myco-curious



Registered: 09/08/12
Posts: 1,827
Loc: "Alice's Wonderland"
Last seen: 9 years, 2 months
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Re: Remove tissue from agar? [Re: RogerRabbit]
#19033315 - 10/25/13 09:39 PM (10 years, 3 months ago) |
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The yellow spots are bacteria, which is much easier to isolate from than mold   http://www.shroomery.org/forums/showflat.php/Number/17451779
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,847
Loc: In the Gills
Last seen: 1 hour, 13 minutes
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Re: Remove tissue from agar? [Re: RogerRabbit]
#19035494 - 10/26/13 12:34 PM (10 years, 3 months ago) |
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Quote:
RogerRabbit said: Send the confirmation email and I'll reinitialize the download.
To the original poster, if all you have is dry tissue you need to get some spores off the gills to germinate. Getting dry mycelium to grow again can be done but it takes a lot more work than that. RR
Ya i deleted the email cause im genius like that lol its ok i will just buy it again. Its not like you havent done enough for me, its very cost effective lol
OP: I missed the part about the original sample being dry flesh (hate it when i do that) RR is right this could be very hard. I think you could easily confuse contam myce with normal myce crossed with bacteria smudges lol your best bet is to use flesh from the gills like he said that will have spores on them. It wont be a clone really but it will live.
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