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magicbroncoride
barbaric neanderthal

Registered: 05/27/13
Posts: 208
Last seen: 8 years, 5 months
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contam timeline
#19030264 - 10/25/13 09:50 AM (10 years, 3 months ago) |
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So my mini mono has triched out before colonization was complete, which is leading me down a couple avenues of thought. In searching through the archives here I ran across some 5 yr old info that says "if your tub contams before colonization your spawn was contaminated" is this still common perception?
This grow was done in a completely different area (different house) with trusted vendor rye berry bags (with ship) with a brand new spore syringe. Innoc was done in a sab after room had been still and sprayed with lysol. 3 bags were innoced and fully colonized with a week of consolidation to see if anything came up. Each one pound bag was then spawned at a 1:1 ratio in a fresh mini tub. Spawn was coir/vermiculite that was pasturized on the stove in a big pot with a recently calibrated thermocouple from work with an eye on temp the whole time.
One week later im at 80 to 90% colonization on all three tubs. Tubs are in 3 separate rooms and temps are staying in the 72-75f range. Tub 1 has maybe a 1/3 turn green while tubs 2 and 3 have green polka dots and maybe a 1 inch x 4inch green strip.
No material used in this grow had ever been used before. Even sab was made fresh for this trial. So what is the consensus of what I am fucking up? How quickly can trich develop? Since this is before full colonization would this be a spawn issue? Im just puzzled as to how this happens. I guess my next step is a laminar flow hood and some agar work. Im just stumped.
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Civ
Pinning



Registered: 10/14/04
Posts: 2,537
Loc: California
Last seen: 6 months, 18 days
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Not enough control - famous of any small tub. Then too much shit in one tub also is a fail, and you over spawned. Step it up to a larger tub- the biomass of your spawn creates heat- and the c02 falls out the bottom, helping draw fresh(er) air in from your top vents. Trich is mostly gonna happen due to poor air flow and medium preparation. Your contamination has ALWAYS been there, based on your timeline, just the way you did it- accelerated it exponentially. Its the whole backbone of the Double and Mono tub! is using the size of the substrate to do the work. Otherwise you just make a bucket of rotten food hoping some shit grows on it 
You cannot go wrong with making to many holes. you can cover up holes after your grow has a foothold - but creating holes in a tub that's colonized sucks. Make sure your bottom holes are below the top of your substrate.
-------------------- "...Gal's seem to hate the thought of blending chicken shit in a blender. So, wash it well afterwards & DON'T tell them..." -Agar
Edited by Civ (10/25/13 10:09 AM)
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Thadeous
On the path

Registered: 08/02/11
Posts: 1,101
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Re: contam timeline [Re: Civ]
#19030393 - 10/25/13 10:30 AM (10 years, 3 months ago) |
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I call into question your pasteurization techniques.. Did you just have you coir/verm in the pot? or in, say, quart jars sitting in water in the pot? If the latter, I redact my statement, if not, well.. this: http://www.shroomery.org/forums/showflat.php/Number/17246844#17246844
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Being as you properly pasteurized CV and it turned green before you introduced fruiting conditions, I'm gonna say it was your spawn.
During colonization of your substrate, I'm presuming you only allowed for GE and not FAE? Do you have a pic of your tub so we can see your set up? I would like to see how your mini was made.
Don't let your spawn consolidate for a week. Spawn as soon as it's 100% colonized. A day or two extra don't hurt, but don't intentionally consolidate.
Learn to use agar so you can make sure your culture is clean.
Learn to prepare your own grains so your mistakes won't be so costly.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
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magicbroncoride
barbaric neanderthal

Registered: 05/27/13
Posts: 208
Last seen: 8 years, 5 months
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Sorry I dont have pics of the tubs as I chucked them immediately. There were 1/2 and 1 inch holes spread vigorously about then taped. These are the small shoebox size tubs that some make a super cake out of. I didn't want to do full size monos as I really depresses me when I lose one and eats up a lot of materials. I followed franks pasturiztion tek exactly. I normally do prepare my own grains (wbs) I was just using the pre made bags to take my grain prep out of the equation, same as the pre made syringe. I figured as many variables that I removed the better. I even considered using the premade substrates but I couldnt bring myself too since I have so much coir and vermic. I will pick up some more full size monos later today and start preparing them.
More bags and fresh syringe are on thier way just to keep same parameters as first test. I might try some agar work next week and see how dirty my spore prints are that I have been collecting.
Edited by magicbroncoride (10/25/13 11:06 AM)
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meticulousity
Stranger


Registered: 08/31/11
Posts: 53
Last seen: 1 year, 11 months
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I used to have a lot of issues with trich when I lived in the country, and it generally did come up earlier rather than later.
This may not be the same issue that you're having but I'll tell you how I fixed mine drastically.
My substrate was the same as yours, coir/verm, but I pasteurized a little differently. I took a 5 gallon bucket, filled it with all of the substrate, then dumped a huge pot of rapidly boiling water into it and let it sit for awhile (2-6 hours). If you have thermal gloves you can just handle the substrate while it's still a bit hot, just watch you don't burn your spawn when you toss it in. So the pasteurization I did a bit differently.
But also, I started adding manure and gypsum to the substrate. The manure was just to add more nutrients, but the gypsum was to control the pH levels better, as the above post stated. Trich flourishes in a different range than psilocybes (i don't remember the numbers so I'm not gonna wing it). Once the cubensis myc starts colonizing, the natural byproducts of the growth changes the pH, allowing the trich a better chance to take hold. The gypsum stabilizes these pH changes a bit.
So between the different pasteurization and different substrate, I was able to control the 'mean green' that just ruins your day when you see it. For more exact details on the amount of gypsum to add, time for pasteurization, etc. there's plenty of teks and threads about it, check it out. Hope that helps!
-------------------- There are 3 types of people in this world. Those who can count, and those who can't.
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