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Offlinelonelyjew
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Registered: 05/02/13
Posts: 300
Last seen: 3 years, 1 month
syringe or my sterilization?
    #19026921 - 10/24/13 05:24 PM (10 years, 3 months ago)

i used two different syringes to knock up 12 jars (six each)

4 jars have visible contams so far and they are all from the same syringe (TRICH GRRR:mad2:)




i used francs WBS jars and pressure cooked them at 15 psi for 90 minutes i let them cool overnight, i sprayed the hell out of my SAB and put the jars and syringed in there and resprayed, i inoculated them and flame sterilized between each jar( after i inoculated them all i realized i didn't use enough and inoculated some more in the first six)

then after a few hours i realized i forgot to shake the syringe and reinoculated them(didn't think it would harm anything considering its an rtv silicone self healing injection port and i followed all the proper sterilization techniques)



and should i separate the 4 contaminated jars or does it not really matter?




oh and this is my first grow so any constructive criticism is welcome!!!


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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Offlinelonelyjew
Stranger


Registered: 05/02/13
Posts: 300
Last seen: 3 years, 1 month
Re: syringe or my sterilization? [Re: lonelyjew]
    #19027758 - 10/24/13 07:29 PM (10 years, 3 months ago)

just identified trich in a 5th jar and i think i see some cobweb on the bottom of the sixth jar it kinda looks like a few strands of poly fill

and the other 6 jars have no growth yet but no contams



so would you say the syringe was bad i would say so but would like an opinion from someone more experienced.


--------------------
Everything I say is a lie, I pretend to do drugs so people will think I'm cool.


:yogabunny:


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