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InvisibleMr. Dabz
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Registered: 05/08/13
Posts: 39
Deleted
    #19026564 - 10/24/13 04:02 PM (11 years, 2 months ago)

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reason... need to restart my life and this is not putting me on a good path >.> later every one!

Edited by Mr. Dabz (03/31/14 10:29 AM)

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Invisibledusttodust


Registered: 11/26/12
Posts: 491
Re: syringe and LC question [Re: Mr. Dabz]
    #19026681 - 10/24/13 04:31 PM (11 years, 2 months ago)

if i understood you right you are worried about the inside part of syringe that is exposed to outside air before you suck in the water right?

you can solve your problem by using new syringe. if you use old ones, sterilize them in aluminium foil and dont take the foil of until you actually use them.

apart from that i want to point out that glc contains LOTS of mycelium and few mold spores or bacteria from air shouldnt cause any harm (unless you actually expose glc to air, than this would be a problem). i also think that it is almost impossible to not have any contams in your "sterile area" , its only about how many of them are present. to sum up what i wanted to say, dont worry about air, if you have clean syringe and if the air isnt rly polluted with many mold spores or bacteria or whatevery you should be fine.

yes, it will work with spore syringe. how did you imagine making lc anyway? and theres a diffrence between GLC and LC , right?

Edited by dusttodust (10/24/13 04:44 PM)

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Offlinehgmstl
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Registered: 10/07/12
Posts: 463
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Last seen: 10 years, 9 months
Re: syringe and LC question [Re: dusttodust]
    #19026690 - 10/24/13 04:33 PM (11 years, 2 months ago)

gtg and agar


--------------------
Yo yo yo

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InvisibleMr. Dabz
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Posts: 39
Re: syringe and LC question [Re: dusttodust]
    #19027337 - 10/24/13 06:29 PM (11 years, 2 months ago)

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reason... need to restart my life and this is not putting me on a good path >.> later every one!

Edited by Mr. Dabz (03/31/14 10:28 AM)

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InvisibleMr. Dabz
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Registered: 05/08/13
Posts: 39
Re: syringe and LC question [Re: Mr. Dabz]
    #19027358 - 10/24/13 06:32 PM (11 years, 2 months ago)

Deleted post
reason... need to restart my life and this is not putting me on a good path >.> later every one!

Edited by Mr. Dabz (03/31/14 10:28 AM)

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