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Offlinesilverstem
Caps & Stems
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Registered: 10/12/13
Posts: 900
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Last seen: 6 years, 5 months
how is this kit? agar
    #19026124 - 10/24/13 02:40 PM (10 years, 3 months ago)

is this a good agar kit?

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Edited by FooMan (10/25/13 08:43 AM)


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Invisiblepoopy mcpooperson
Pogonophobia
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Registered: 10/17/13
Posts: 124
Re: how is this kit? agar [Re: silverstem]
    #19026173 - 10/24/13 02:51 PM (10 years, 3 months ago)

i just pieced mine together from a sponsor. its a lot cheaper. also check out franks agar teks. its a lot cheaper to just buy agar agar from a chinese market and light malt extract from a beer supply store and mix it up. in my experience so far agar from a supplier is the most expensive part. check out the sponsors and piece together your own "kit" i can give you my list if youd like.


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Offlinesilverstem
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Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 6 years, 5 months
Re: how is this kit? agar [Re: poopy mcpooperson]
    #19026180 - 10/24/13 02:52 PM (10 years, 3 months ago)

i would love that.. please pm me it.


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Offlinellama_police
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Registered: 02/20/13
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Re: how is this kit? agar [Re: silverstem]
    #19026200 - 10/24/13 02:55 PM (10 years, 3 months ago)

I get my malt extract from the same asian supermarket as I get the agar powder. I just use 1/4 pint jars too. Doesn't get much cheaper than that


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Invisiblepoopy mcpooperson
Pogonophobia
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Registered: 10/17/13
Posts: 124
Re: how is this kit? agar [Re: silverstem]
    #19026254 - 10/24/13 03:04 PM (10 years, 3 months ago)

ill just post it on here so others can see it too.

1a. RRs video on how to prepare agar
1b. sterile petri dishes
2. agar- i used the MEA agar
Malt Extract Agar (3%)(MEA)(General Microbiology)(FDA M93)
-----------------------------------------------------------------
30 g Malt extract (beer supply store)
20 g Agar (chinese supermarket)
1 L Distilled water

here are freds other recipes though:
http://www.shroomery.org/forums/showflat.php?Cat=&Board=59&Number=3077006&PHPSESSID=&fpart=1#3077006

3. SAB (still air box)- http://www.shroomery.org/forums/showflat.php/Number/14722730#14722730

4. surgical blade or exacto knife
5. alcohol flame to sterilize knife
6. gloves
7. tyvek sleeves
8. parafilm
9. bottle or jar to cook agar
10. steady hands

hope this helps.


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Invisiblepoopy mcpooperson
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Registered: 10/17/13
Posts: 124
Re: how is this kit? agar [Re: poopy mcpooperson]
    #19026331 - 10/24/13 03:18 PM (10 years, 3 months ago)

also, depending whether or not you are using a MS solution syringe for isolation or a print you would need to get an inoculation loop. i made one myself out of a paper clip and a pair of pliers. just heat the shit out of it.


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Offlinesilverstem
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Registered: 10/12/13
Posts: 900
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Last seen: 6 years, 5 months
Re: how is this kit? agar [Re: poopy mcpooperson]
    #19029464 - 10/25/13 03:01 AM (10 years, 3 months ago)

im using an isolate.


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