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Offlineshqhgh123
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Registered: 09/20/13
Posts: 10
Last seen: 10 years, 3 months
Should I make a LC or do MS inoculation?
    #19025826 - 10/24/13 01:47 PM (10 years, 3 months ago)

I have spore syringes but will get the substrate in two days. I can go ahead and buy stuff for LC now.

Since LC takes a week to germinate anyway, is it worth it to make a LC at this point?


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OfflinePussyFart
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Registered: 04/08/12
Posts: 22,502
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Last seen: 1 month, 10 days
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Re: Should I make a LC or do MS inoculation? [Re: shqhgh123]
    #19025892 - 10/24/13 02:01 PM (10 years, 3 months ago)

It's hardly ever worth it to make an LC from spores.

Spores can take more than 2 weeks to germinate in some cases, regardless of the medium they are on, then you would still have to wait for the LC to colonize, before shooting up test jars and growing them out.

Skip LCs and research agar and G2G transfers.

It's a much more promising path.


Edited by PussyFart (10/24/13 02:01 PM)


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Offlinerumfor69
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Registered: 08/05/11
Posts: 6,846
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Re: Should I make a LC or do MS inoculation? [Re: PussyFart]
    #19025961 - 10/24/13 02:13 PM (10 years, 3 months ago)

:whathesaid:
spore to agar first so you can see the clean myce first and isolate 
clean mcye to other dishes.


--------------------

Links For Beginners And Beyond:mspoil:


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Offlineshqhgh123
Stranger
Registered: 09/20/13
Posts: 10
Last seen: 10 years, 3 months
Re: Should I make a LC or do MS inoculation? [Re: rumfor69]
    #19025968 - 10/24/13 02:15 PM (10 years, 3 months ago)

Ookay thanks guys. I decided to stick with just MS inoculation.


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