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OfflineKalypto
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Can you smell a jar before G2G
    #19024738 - 10/24/13 10:07 AM (10 years, 3 months ago)

Its nice to smell them so I dont contaminate the G2G , but I dont wanna contam the jar just for a smell


Should I be smelling them before G2G


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Re: Can you smell a jar before G2G [Re: Kalypto]
    #19024749 - 10/24/13 10:09 AM (10 years, 3 months ago)

How are you going to smell them without introducing contams? Try to smell through the GE port after you shake it. That's the best you can do. Smell after you g2g and if it smells bad toss all the jars you just g2g'ed


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OfflineKalypto
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Re: Can you smell a jar before G2G [Re: bodhisatta]
    #19024769 - 10/24/13 10:14 AM (10 years, 3 months ago)

I hear people say fully colonized jars are pretty much contam resistant


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Re: Can you smell a jar before G2G [Re: Kalypto]
    #19024775 - 10/24/13 10:16 AM (10 years, 3 months ago)

The fully colonized one is.  But whatever contams land on the grains in that jar willbe transfers to the sterile jars you are about to g2g so, no bueno.


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Re: Can you smell a jar before G2G [Re: Kalypto]
    #19024777 - 10/24/13 10:16 AM (10 years, 3 months ago)

Fully colonized PF cakes and Fully colonized substrates are contam resistant. Your grain jar might already have a contam in it. Besides opening it in open air puts contams on it that won't contaminate the original grain but you'll have spores that will make it to the uncolonized grain in the new jars and they will contam...


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Re: Can you smell a jar before G2G [Re: Kalypto]
    #19024783 - 10/24/13 10:17 AM (10 years, 3 months ago)

Without opening my grain jars I can smell their fierce mushroom smell that permeates through the GE hole.


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Re: Can you smell a jar before G2G [Re: DeadPhan]
    #19024786 - 10/24/13 10:18 AM (10 years, 3 months ago)

With that however, I work in front of a flow hood soi can usually smell once opening anyway from the laminar flow bringing that good smell towards me. I also tend to give the lid a sniff.  I'd imagine if you could get the lid out safe enough from a SAB you could try that


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OfflineKalypto
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Re: Can you smell a jar before G2G [Re: DeadPhan]
    #19024808 - 10/24/13 10:23 AM (10 years, 3 months ago)

Quote:

Sagescruffy said:
Without opening my grain jars I can smell their fierce mushroom smell that permeates through the GE hole.



Will try

Quote:

DeadPhan said:
With that however, I work in front of a flow hood soi can usually smell once opening anyway from the laminar flow bringing that good smell towards me. I also tend to give the lid a sniff.  I'd imagine if you could get the lid out safe enough from a SAB you could try that



Soon Ill have a LFH hopefully




So its ok for contams to get on it provided there is something else there

So thats why open air doesnt matter for spawning tubs , cause everything is already occupied?


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Re: Can you smell a jar before G2G [Re: Kalypto]
    #19024836 - 10/24/13 10:29 AM (10 years, 3 months ago)

Contams can touch fully colonized substrate. The target jars for g2g are not fully colonized so the fully colonized jar that you open to smell gets some contams in it from open air they get to your uncolonized grain and boom contam. The master jar wont contam because the myc has occupied all the grains and this is also correct of spawning. We pasteurize the substrate so that thermophilic bacteria have occupied it so trich and cobweb cant take hold before the myc from the spawn colonizes it.

You might as well treat a master grain jar as uncolonized because it is linked to uncolonized media.


Edited by Trusted cuItivator (10/24/13 10:29 AM)


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OfflineKalypto
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Re: Can you smell a jar before G2G [Re: bodhisatta]
    #19024845 - 10/24/13 10:31 AM (10 years, 3 months ago)

Cool Thats what I started to figure out


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Re: Can you smell a jar before G2G [Re: Kalypto]
    #19024905 - 10/24/13 10:45 AM (10 years, 3 months ago)

Not just something else, it has to be the right something else.

Most substrates are considered low nutrition as compared to grains. We can just pasteurize substrate and the thermophilic bacteria left will help to protect the sub while your mycelium colonizes. Even when something lands on it, it takes time for germination and colonization of the contam to occur. This is at least one reason why you can't just put spores on pasteurized substrate and expect success. But, eventually, other contaminants will take over.

Grains have to be sterilized and kept in sterility or else everything that lands on it will grow. They are so highly nutritious, that if you just pasteurized them, they will show contams very quickly.


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OfflineKalypto
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Re: Can you smell a jar before G2G [Re: SpitballJedi]
    #19024969 - 10/24/13 10:58 AM (10 years, 3 months ago)

Of course the right something being mycellium or thermophillic bacteria?


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Re: Can you smell a jar before G2G [Re: Kalypto]
    #19024981 - 10/24/13 11:01 AM (10 years, 3 months ago)

yes


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OfflineKalypto
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Re: Can you smell a jar before G2G [Re: SpitballJedi]
    #19024998 - 10/24/13 11:05 AM (10 years, 3 months ago)

Kool ,Its nice to get back to growing and learning


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