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InvisibleNeller
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Registered: 10/18/13
Posts: 63
Loc: kentucky
Reishi lc contam?
    #19022084 - 10/23/13 09:30 PM (10 years, 3 months ago)

This is my first post and my first attempt at making an lc. The Shroomery is a great resource to have. Thanks to everyone for making it what it is.

Is it normal for these blackish specks to be on the outer edge of the mycelium like this?



LC is 4% light karo 96% water. the other jar doesn't look like this one but I had them in different conditions. The other had less growth with no black specks. Inoculated both with reishi lc syringe on 10/12/13.

Also how important is GE on LC like this. I made a SHIP with silicone on the jar lid and used a syringe with poly-fil (didn't have cotton) and alcohol to equalize pressure then inoculated. Could this cause significant problems with growth?

Thanks, Neller


--------------------
"If there’s one generational difference I notice between my parents’ generation and mine, is that my generation values time over money. And not because we’re lazy either, but because we’re not willing to trade time with the people we love most for a gold watch at retirement."
-BRETT

Reaching for heaven is what I'm on earth to do.



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OfflineForresterM
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Re: Reishi lc contam? [Re: Neller]
    #19022296 - 10/23/13 10:05 PM (10 years, 3 months ago)

That picture doesn't help much, but black specks are usually a bad thing.  Reishi mycelium does not have black specs.  Probably contamination.

Quote:

Neller said:
I made a SHIP with silicone on the jar lid and used a syringe with poly-fil (didn't have cotton) and alcohol to equalize pressure then inoculated. Could this cause significant problems with growth?




I'm completely lost as to what you mean by this.  Equalize pressure?  What exactly did you do? :confused:


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Offlinedeadmandave
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Re: Reishi lc contam? [Re: Forrester]
    #19024355 - 10/24/13 07:50 AM (10 years, 3 months ago)

if a jar of liquid is pressure cooked without any holes for GE it will create a vacuum as it cools. a blank syringe stuffed with polyfil is stuck into the injection port so that the pressure can equalize and hopefully not suck in contams.

In the future use polyfil as a filter on your jars, or get filter disks :lol: GE is important for mycelial growth.

I agree with forrester, reishi shouldnt have black spots, looks like a contam.


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OfflineForresterM
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Re: Reishi lc contam? [Re: deadmandave]
    #19024384 - 10/24/13 08:04 AM (10 years, 3 months ago)

Quote:

deadmandave said:
if a jar of liquid is pressure cooked without any holes for GE it will create a vacuum as it cools. a blank syringe stuffed with polyfil is stuck into the injection port so that the pressure can equalize and hopefully not suck in contams.




Ah, now I get it! :thumbup:

wouldn't it just be easier to put a GE hole w/filter?


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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InvisibleNeller
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Registered: 10/18/13
Posts: 63
Loc: kentucky
Re: Reishi lc contam? [Re: Forrester]
    #19027476 - 10/24/13 06:51 PM (10 years, 3 months ago)

I didn't put a filter patch on it because I am clumsy and was afraid I would jostle the jars and get some lc on the patch causing a contam.  Plus my lack of experience with the technique of lc.

So mycelia produces co2. Is this similar to yeast. I just find it hard to comprehend that something submerged in a liquid can benefit from gas exchange above the liquid. Unless it creates co2 and carbonates the lc without ge. Is this line of thinking way off target?

Thank you for your replies. Not the worst news about the contam. I have another for the time being and a few grain jars.


--------------------
"If there’s one generational difference I notice between my parents’ generation and mine, is that my generation values time over money. And not because we’re lazy either, but because we’re not willing to trade time with the people we love most for a gold watch at retirement."
-BRETT

Reaching for heaven is what I'm on earth to do.



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Offlinedeadmandave
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Re: Reishi lc contam? [Re: Neller]
    #19029324 - 10/25/13 01:39 AM (10 years, 3 months ago)

im guessing that the mycelium breathes above the surface of the water, like coming up for air. maybe that's partly why magnetic stir sticks are effective, agitation allowing more oxygen in contact with mycelium.

a mystery to me


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