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Monkeysuckerpunch
Stranger
Registered: 08/19/13
Posts: 56
Last seen: 3 years, 5 months
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Easy agar question, but can't find answer
#19021823 - 10/23/13 08:48 PM (10 years, 3 months ago) |
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I'm about to start using agar for the first time and I want to use a piece of the actual mushroom to inoculate it. My questions:
1. What part of the actual mushroom do I use? Piece of the innards? The gills? What exactly??
2. My understanding is that once your mycelium starts producing shrooms, one would want to take a piece of the mushroom which you most want to replicate and use that in your agar and then select a dikaryotic (or, ropey-mycelium) strain from that piece. That is what would give you the best chance of producing another mycelium that will produce similar shrooms. Is this correct? Even close?
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 12 hours, 57 minutes
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Using a piece of flesh from the inside of the stem is best.
Cloning a mushroom is a good way to get similar results in the future
like size, and pinset, potency sometimes.
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Re: Easy agar question, but can't find answer [Re: rumfor69]
#19022185 - 10/23/13 09:47 PM (10 years, 3 months ago) |
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How the mycelium looks on agar has mostly to do with th nutrient content of the agar itself. Taking clones from different areas of the mushroom, or selecting different mycelial looks isn't generally necessary. Just avoid the gill area in general or you'll be playing with the randomnly selected nature of spores. The important part is to be sterile, and clean the outer parts of the mushroom. Flame the scalpel red hot. Two scalpels is helpful. Clean yourself as well, for the cultivator is the number one source of contamination with this procedure
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PsiLisaBin
Stranger<<<Strangest

Registered: 07/11/13
Posts: 128
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Re: Easy agar question, but can't find answer [Re: Oeric McKenna]
#19022284 - 10/23/13 10:03 PM (10 years, 3 months ago) |
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I would just take the mushie I want to clone and rip the stem in half (grab it by the base and pull it apart), then scrape some of the tissue from inside the stem onto your petri. All in your still air box of course and flame your scalpel & all that jazz.
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Monkeysuckerpunch
Stranger
Registered: 08/19/13
Posts: 56
Last seen: 3 years, 5 months
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Re: Easy agar question, but can't find answer [Re: PsiLisaBin]
#19025439 - 10/24/13 12:38 PM (10 years, 3 months ago) |
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Thanks for the direction everybody!
Now just to find scalpels irl!
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JohnnieYen
Okay



Registered: 03/15/11
Posts: 3,529
Loc: City Z
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in a pinch you could heat sterilize long tweezers or a razorblade held with pliers/vicegrips.
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rumfor69
Bodhicitta Cultivator



Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 12 hours, 57 minutes
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Re: Easy agar question, but can't find answer [Re: JohnnieYen]
#19025918 - 10/24/13 02:05 PM (10 years, 3 months ago) |
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just buy an exacto blade scalpel from the hardware store
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Oeric McKenna
LIFE CAPS


Registered: 06/15/12
Posts: 5,318
Loc: Babylon
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Re: Easy agar question, but can't find answer [Re: rumfor69]
#19034304 - 10/26/13 05:25 AM (10 years, 3 months ago) |
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_this guys signature^^^....SO DOPE!
Yeah that's what I always did in the pre-scalpel days. Go to wally world or something and get the x-acto set.
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