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Offlinesilverstem
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Registered: 10/12/13
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Last seen: 6 years, 6 months
2nd gow...log * 1
    #19013629 - 10/22/13 12:51 PM (10 years, 4 months ago)

so while i wait for my first grow to colonize i decided to start my 2nd grow

i decided to go with pp5 plastic this time..


i have wayyy too much verm left lol.



im using a 2:1:1 verm:brf:water mixture.


I fill my plastic tubs 7/8 full then add a dry verm layer


after i put two holes in the top i covered with foil


NOW its ready for sterilization. i steam sterilize for 2 hours.


im using a diluted spore syringe. they claim its paurto rican but who cares about "strains". after my first grow is done i will pick an isolate.


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InvisibleDebuteMachine

Registered: 09/29/06
Posts: 6,457
Re: 2nd gow...log [Re: silverstem]
    #19013702 - 10/22/13 01:08 PM (10 years, 4 months ago)

very nice, can't wait to see how the Tupperware turns out  :awebig:


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Offlinesilverstem
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Re: 2nd gow...log [Re: DebuteMachine]
    #19013723 - 10/22/13 01:13 PM (10 years, 4 months ago)

DynGBreeD actually suggested the tupperware.


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InvisibleDebuteMachine

Registered: 09/29/06
Posts: 6,457
Re: 2nd gow...log [Re: silverstem]
    #19013758 - 10/22/13 01:18 PM (10 years, 4 months ago)

you did skip a picture of where and how you inoculated them though. yes, i can imagine, but this is science. i want evidence.

:awetongue:


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Offlinesilverstem
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Re: 2nd gow...log [Re: DebuteMachine]
    #19013768 - 10/22/13 01:20 PM (10 years, 4 months ago)

lol they are still steam sterilizing. still got another hour to go.


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InvisibleDynGBreeD
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Registered: 01/15/11
Posts: 3,639
Re: 2nd gow...log [Re: silverstem]
    #19014414 - 10/22/13 04:06 PM (10 years, 4 months ago)

Cool Stuff bro! But 24 ounces?? Those are some big cakes.
You aughta make up some small ones too. I've never had
much luck with anything over 12. Plus the larger the cake,
the longer the colonization time.
I would add an extra inoculation hole dead center of the tubs. (If it were "I")
Reason being because I've had larger ones to appear finished, even after 1-2 week
consolidation. For the middle to be un-colonized fully.

I've used some that are those size, but instead I spawned
four ounce cakes (1/4th pints) to bulk and made little bulk
tubs for a SGFC instead of my greenhouse.


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Edited by DynGBreeD (10/22/13 04:08 PM)


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Offlinesilverstem
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Re: 2nd gow...log [Re: DynGBreeD]
    #19017049 - 10/22/13 11:43 PM (10 years, 4 months ago)

i dont wanna add anymore inoculation holes. that will leave more room for contams. i think il be fine but advice is advice and il take yours lol. i put 4 inoculation points in two of them.

pics tonight.


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Edited by silverstem (10/22/13 11:54 PM)


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InvisibleDynGBreeD
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Registered: 01/15/11
Posts: 3,639
Re: 2nd gow...log [Re: silverstem]
    #19017165 - 10/23/13 12:13 AM (10 years, 4 months ago)

I'll be waiting! Did you add the Vermiculite barrier?
I know even though born aren't needed, I use the
Vermiculite barrier and micro pore tape before sterilizing.

I simply inoculate through the tape, still gets plenty of
gas exchange. You could use the needle to make the fifth
hole. Carefully of course, Then apply a piece of Micro pore tape.

Either way by using such large tubs you increase the chance
for contaminates. It's not a sure failure though. Sometimes
the substrate settles so much I the bottom that the mycelium
had trouble colonizing.

Either way bro, it's your grow. Hope I'm helpful.
:awethumb:


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Offlinesilverstem
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Re: 2nd gow...log [Re: DynGBreeD]
    #19017276 - 10/23/13 12:54 AM (10 years, 4 months ago)

yea i have a dry verm layer. u put ur tape on before sterilizing ? i do it after inoculation. i feel good about this grow those containers are a little bigger then the jars but because of its shape it has alot more dryverm layer on it. so because of its top surface layer i added a full cup of dry verm on top instead of the 1/2 cup i would add in a jar making the cakes volume less then a quart jars. and weve all seen quart jars colonize.


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InvisibleDynGBreeD
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Registered: 01/15/11
Posts: 3,639
Re: 2nd gow...log [Re: silverstem]
    #19017306 - 10/23/13 01:13 AM (10 years, 4 months ago)

Yeah before inoculation, helps keep the moisture out a little
I think. Just one of those effortless steps that not necessarily
needed but doesn't hurt me any to take.
Your method is fine though, after doing this hobby for a few
years you kinda get set in your own ways. Because after all
you know what works Best for you right?
Any who, sorry for yakkin your ear off....
BTW Rockin avatar and signature. :headbang3:


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InvisibleDebuteMachine

Registered: 09/29/06
Posts: 6,457
Re: 2nd gow...log [Re: silverstem]
    #19017346 - 10/23/13 01:34 AM (10 years, 4 months ago)

Quote:

silverstem said:
lol they are still steam sterilizing. still got another hour to go.





lol, wow. i really jumped the gun on that one.


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Offlinesilverstem
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Re: 2nd gow...log [Re: DebuteMachine]
    #19017347 - 10/23/13 01:36 AM (10 years, 4 months ago)

yea just inoculated like 20 min ago.


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Offlinesilverstem
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Registered: 10/12/13
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Re: 2nd gow...log [Re: silverstem]
    #19034872 - 10/26/13 10:03 AM (10 years, 3 months ago)

okay so i used a diluted mixture from one syringe into 3 and i see life.

all 5 containers have healthy myc.




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InvisibleDynGBreeD
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Registered: 01/15/11
Posts: 3,639
Re: 2nd gow...log [Re: silverstem]
    #19035307 - 10/26/13 11:49 AM (10 years, 3 months ago)

:congrats:

You're doin work, sorry if I missed it
how long since inoculation and visible growth?

:awepreciation:


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Offlinesilverstem
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Re: 2nd gow...log [Re: DynGBreeD]
    #19035424 - 10/26/13 12:17 PM (10 years, 3 months ago)

4 days. and all have growth from ms.. its myc too no contams yet in any of my grows.


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InvisibleDebuteMachine

Registered: 09/29/06
Posts: 6,457
Re: 2nd gow...log [Re: silverstem]
    #19042266 - 10/27/13 07:20 PM (10 years, 3 months ago)

and the wait begins :bigjoint:


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Offlinesilverstem
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Re: 2nd gow...log [Re: DebuteMachine]
    #19048828 - 10/28/13 08:57 PM (10 years, 3 months ago)

UPDATE:

Lots of growth.


and thats 6 days from inoculation from ms.


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Edited by silverstem (10/28/13 08:59 PM)


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Offlineroot9
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Registered: 09/07/13
Posts: 47
Last seen: 8 years, 5 months
Re: 2nd gow...log [Re: silverstem]
    #19048884 - 10/28/13 09:06 PM (10 years, 3 months ago)

Quote:

silverstem said:



.




You appear to have a large shrub growing from your forearm lol

I'm curious to see how these turn out. Is your plan to just take the lid off, dump or hydrate the dry verm, then set them into a FC?


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Offlinesilverstem
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Re: 2nd gow...log [Re: root9]
    #19048974 - 10/28/13 09:23 PM (10 years, 3 months ago)

lol no. spawning to coir in a monotub im not wasting time with a fc. straight to bulk growing. ive done my research.


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InvisibleDebuteMachine

Registered: 09/29/06
Posts: 6,457
Re: 2nd gow...log [Re: silverstem]
    #19054097 - 10/29/13 08:25 PM (10 years, 3 months ago)

can i request a picture from the top of the tupperware? i want to see where the holes are at. excuse me for being a noob and needing seemingly boon details.


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