Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   PhytoExtractum Buy Bali Kratom Powder   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinejaycrayzy
Xombie Boy
 User Gallery

Registered: 08/22/13
Posts: 73
Loc: Under the bed
Last seen: 1 year, 1 month
I want to see picture of strain isolated grows vs multispore * 1
    #19016329 - 10/22/13 09:22 PM (10 years, 3 months ago)

I have tried looking for grows from multispore vs isolated strains but have not seen anything. Of course I have seen many multispore grows but if any of you have pictures of grows using agar, I would really like to see them. Also, do you who use agar think it is worth it? I am about to start the second flush on my first grow and I'm really interested in strain isolation and getting away from multispore inoculation.


--------------------
:zombie5:Jay Kay the Xombie:zombie5:


Extras: Filter Print Post Top
InvisibleLiquidGlass
Glass Blower
 User Gallery

Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
Re: I want to see picture of strain isolated grows vs multispore [Re: jaycrayzy] * 1
    #19016345 - 10/22/13 09:24 PM (10 years, 3 months ago)

Agar is totally worth it . . .


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


Extras: Filter Print Post Top
InvisiblePirateSwazey
 User Gallery


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: I want to see picture of strain isolated grows vs multispore [Re: LiquidGlass] * 1
    #19016511 - 10/22/13 09:52 PM (10 years, 3 months ago)

Quote:

LiquidGlass said:
Agar is totally worth it . . .





--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



Extras: Filter Print Post Top
InvisibleViolet
 User Gallery


Registered: 12/06/11
Posts: 4,205
Re: I want to see picture of strain isolated grows vs multispore [Re: LiquidGlass] * 1
    #19016561 - 10/22/13 09:57 PM (10 years, 3 months ago)

"Getting away from multispore" is, for me, the first part of the grow.  I don't waste expanding something unknown when a little culturing can be selective.
Seriously only like 1 out of 1000 (not a serious stat) genes are worth even testing to compare to each other.  Cloning vastly reduces culture work while bringing a wider smile with test results.  With certain culturing methods you can even yield while you sort-out and pick genes. I'm currently building a sweet thread about this now that will be posted within a few days!


I'll grab a few multi-spore and several isolate grow pics from my photos, for fun...

MS



ISOLATES  (An isolate doesn't mean a good isolate, and most of my photos so far are of so-so clones, fyi)
Rows are same isolate.










Galindoi clone isolate straight-grain:


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Edited by Violet (10/22/13 10:05 PM)


Extras: Filter Print Post Top
Invisiblepoopy mcpooperson
Pogonophobia
 User Gallery


Registered: 10/17/13
Posts: 124
Re: I want to see picture of strain isolated grows vs multispore [Re: Violet] * 1
    #19016835 - 10/22/13 10:51 PM (10 years, 3 months ago)

that is an amazing difference. i have never grown one single mushroom but i am starting off with agar right now and am going to try and isolate before i ever start my jars. im going to do a monotub and see if i can get these double digit ounces i keep reading about. im talking amazon rainforest canopy.


--------------------
love and laughter  :laugh:


Extras: Filter Print Post Top
Offlinejackalope9517
(/*o*)/


Registered: 01/16/13
Posts: 380
Last seen: 9 years, 8 months
Re: I want to see picture of strain isolated grows vs multispore [Re: poopy mcpooperson] * 1
    #19016910 - 10/22/13 11:05 PM (10 years, 3 months ago)

Shit I'm doing an MS bulk grow that I've put my heart and soul into.. and now it's fruiting small/thin pins and I'm worried. Damnit lol..


Extras: Filter Print Post Top
InvisibleLiquidGlass
Glass Blower
 User Gallery

Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
Re: I want to see picture of strain isolated grows vs multispore [Re: jackalope9517] * 1
    #19017421 - 10/23/13 02:15 AM (10 years, 3 months ago)

Quote:

jackalope9517 said:
Shit I'm doing an MS bulk grow that I've put my heart and soul into.. and now it's fruiting small/thin pins and I'm worried. Damnit lol..




Soak it and the next flush will be a lot bigger


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


Extras: Filter Print Post Top
Invisibleuncle_rico
my own worst enemy
Male User Gallery

Registered: 03/28/06
Posts: 2,664
Re: I want to see picture of strain isolated grows vs multispore [Re: jaycrayzy] * 1
    #19017431 - 10/23/13 02:20 AM (10 years, 3 months ago)

MS

Isolate


--------------------


Extras: Filter Print Post Top
Invisiblebarong
Nada
 User Gallery

Registered: 07/24/11
Posts: 666 666 Posts!
Re: I want to see picture of strain isolated grows vs multispore [Re: poopy mcpooperson] * 1
    #19017457 - 10/23/13 02:35 AM (10 years, 3 months ago)

Quote:

poopy mcpooperson said:
that is an amazing difference. i have never grown one single mushroom but i am starting off with agar right now and am going to try and isolate before i ever start my jars. im going to do a monotub and see if i can get these double digit ounces i keep reading about. im talking amazon rainforest canopy.




Someone correct me if I'm wrong, but doesn't it take a few generations on agar to isolate a strain by mycelium transfer? And wouldn't a better method be to grow out a multispore flush, select some of the best fruits, and take a tissue culture from that, for agar transfer, and therefore an instant isolate?


Extras: Filter Print Post Top
InvisibleLiquidGlass
Glass Blower
 User Gallery

Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
Re: I want to see picture of strain isolated grows vs multispore [Re: barong] * 1
    #19017487 - 10/23/13 03:00 AM (10 years, 3 months ago)

One MS mushroom could contain multiple sub-strains of genetics, so you would need a couple transfers anyway. But I like to grow spores on agar, transfer to grain, use that grain to make more jars, grow a tub, then pick the one I like, then clone that to agar a few times to get an isolate.

That way you know you have a fruiting strain before you isolate


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
Trusted Cultivator
OG Cultivator
Re: I want to see picture of strain isolated grows vs multispore [Re: barong] * 1
    #19017763 - 10/23/13 06:47 AM (10 years, 3 months ago)

Quote:

barong said:

Someone correct me if I'm wrong, but doesn't it take a few generations on agar to isolate a strain by mycelium transfer? And wouldn't a better method be to grow out a multispore flush, select some of the best fruits, and take a tissue culture from that, for agar transfer, and therefore an instant isolate?




Wrong on both counts.  It takes a few transfers, not generations to isolate strains.

Cloning is never better than isolation due to the increased number of cell divisions which have already occurred.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Offlinejackalope9517
(/*o*)/


Registered: 01/16/13
Posts: 380
Last seen: 9 years, 8 months
Re: I want to see picture of strain isolated grows vs multispore [Re: LiquidGlass] * 1
    #19018166 - 10/23/13 09:48 AM (10 years, 3 months ago)

Quote:

LiquidGlass said:
Quote:

jackalope9517 said:
Shit I'm doing an MS bulk grow that I've put my heart and soul into.. and now it's fruiting small/thin pins and I'm worried. Damnit lol..




Soak it and the next flush will be a lot bigger



Well, earlier people were saying my substrate was too wet! And I have heard of people not even dunking for the next flush and sometimes only misting and it turns out really well for them. Though my biggest pin currently got wider in the middle but now it's growing thinner at the base again. Silly little pin. What is that fella' doin'? :lol:

Though if i'm not mistaking, light also helps a lot with the density of the fruits. Good thing i've got that down. :P


Edited by jackalope9517 (10/23/13 09:49 AM)


Extras: Filter Print Post Top
InvisibleViolet
 User Gallery


Registered: 12/06/11
Posts: 4,205
Re: I want to see picture of strain isolated grows vs multispore [Re: RogerRabbit] * 1
    #19018185 - 10/23/13 09:52 AM (10 years, 3 months ago)

Quote:

RogerRabbit said:
Cloning is never better than isolation due to the increased number of cell divisions which have already occurred.
RR



Unless your culturing method allows a clone with just a single dish's worth of radial expansion...


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
InvisibleinskiM
Cortinariologist
Male User Gallery


Registered: 02/28/06
Posts: 5,720
Trusted Cultivator
Re: I want to see picture of strain isolated grows vs multispore [Re: jaycrayzy] * 1
    #19018896 - 10/23/13 11:31 AM (10 years, 3 months ago)

Quote:

jaycrayzy said:
I have tried looking for grows from multispore vs isolated strains but have not seen anything. Of course I have seen many multispore grows but if any of you have pictures of grows using agar, I would really like to see them. Also, do you who use agar think it is worth it? I am about to start the second flush on my first grow and I'm really interested in strain isolation and getting away from multispore inoculation.



Here is my work with multi spore cultures verses cloned cultures.
The species experimented with is Psilocybe makarorae
http://www.shroomery.org/forums/showflat.php/Number/18170098/page/6


--------------------


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   PhytoExtractum Buy Bali Kratom Powder   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Is there a strain isolation tek? thecannuck 3,616 2 12/19/02 07:17 AM
by Raadt
* fav. strain to grow Rashiki 2,890 11 10/03/01 02:19 PM
by Fried Brains
* strain isolation effect on yield Dorin 2,435 6 11/16/01 04:17 PM
by Olgualion
* Strain Isolation Anonymous 1,757 4 11/14/02 11:17 AM
by tripndicular
* Strain isolation, and agar cultures amanmusk 2,299 2 03/25/02 10:38 PM
by HisStudent
* best begginer strain-quick grow Phish_shroom 1,763 9 12/03/02 04:08 PM
by Anonymous
* Re: Best strains to grow? 3Mshroom 2,686 4 06/01/00 08:41 AM
by Anonymous
* Re: Can you all suggest a strain to grow? puscle 872 3 03/27/01 10:26 AM
by Ryche Hawk

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
6,782 topic views. 50 members, 307 guests and 47 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.006 seconds on 12 queries.