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OfflineKalypto
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Registered: 09/19/12
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Problems : Tubs wont pin , Contam starting to take over
    #19014337 - 10/22/13 03:49 PM (10 years, 4 months ago)

Ive had a set of tubs in fruiting conditions for 4-5 days with no pins about 3 days ago I increased air flow
Two days ago I reduced polyfil
This morning I put the fan on contantly insread of 30 on 90 off to try and get it started

Instead I have NO pins and Im getting Contamination

Possible reason that im thinking

I should have used bleach instead of alcohol to clean tubs

I didnt clean pillow case properly between pasteurization

I pasteurized wrong

Any tips or questions let me know


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InvisibleKhii Khwaay
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014374 - 10/22/13 03:57 PM (10 years, 4 months ago)

Hard to say without pics.

What tek did you follow?


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InvisibleThadeous
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014375 - 10/22/13 03:57 PM (10 years, 4 months ago)

Was the substrate 100% colonized when introduced to fruiting conditions?


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OfflineKalypto
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Khii Khwaay]
    #19014389 - 10/22/13 04:01 PM (10 years, 4 months ago)



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OfflineKalypto
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014396 - 10/22/13 04:02 PM (10 years, 4 months ago)

It wasnt SOLID white but there was white covering everything You could see a brown tint through some but that was only like 2 of the 6


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InvisibleKhii Khwaay
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014417 - 10/22/13 04:06 PM (10 years, 4 months ago)

What contamination do you see there? They kinda look like they're just doing their thing...

Did you dial in your monos like a boss as per Frank's instructions? Personally, I'd like to see the poly stuffed a lot more loosely in the top holes.


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OfflineKalypto
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Khii Khwaay]
    #19014435 - 10/22/13 04:09 PM (10 years, 4 months ago)

Quote:

Khii Khwaay said:
What contamination do you see there? They kinda look like they're just doing their thing...

Did you dial in your monos like a boss as per Frank's instructions? Personally, I'd like to see the poly stuffed a lot more loosely in the top holes.






Had mad trich in one tube . took that bitch outside and scrapped it

I have not dialed them in , I just moved so im starting back up again
Also I was waiting till my room mate moved out so I could have a full room to get the specifics down but since it seems to be hurting me ill do it now

Do you have a link?


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InvisibleKhii Khwaay
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014447 - 10/22/13 04:12 PM (10 years, 4 months ago)



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InvisibleThadeous
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014456 - 10/22/13 04:15 PM (10 years, 4 months ago)

hmph, the look nicely colonized as far as I can tell.  What contaminate did you get?  Trich?  If so, it can be near impossible to spot before it sporelates.  (Starts off white then turns green during and after sporulation.

If that is the case, I would look towards you pasteurization methods, as even partial sterilization is worse than an unpasteurized substrate.  I use this tek with 100% (so far) success rates.  http://www.shroomery.org/forums/showflat.php/Number/17246844/fpart/1/vc/1


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OfflineKalypto
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Khii Khwaay]
    #19014467 - 10/22/13 04:18 PM (10 years, 4 months ago)

Off to work

I think my method is decent I dont know if its perfect but I just vent steam from my PC into a tub with a pillowcase not touching any side holding my substrate , I let It reach about 180 then hold for 2-3 hours


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InvisibleSpitballJedi
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014480 - 10/22/13 04:23 PM (10 years, 4 months ago)

4-5 days without pins is not that long.

I seriously doubt any of your tub dialing had anything to do with it. I have left the bottom holes un-pollied before and got green mold around those areas pretty quick though.

What is your substrate recipe? If it was anything other than CVG, then it's either your pasteurization tek or spawn contams.

If it was just CVG, then it is more likely your spawn.


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InvisibleThadeous
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014488 - 10/22/13 04:25 PM (10 years, 4 months ago)

180 is way too high.  Proper pasteurization is 160 for 90 minutes.  You are sterilizing your substrate.


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OfflineKalypto
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: SpitballJedi]
    #19014495 - 10/22/13 04:27 PM (10 years, 4 months ago)

CVG?I dont know what that is

Im gonna hope im lucky on this and say That i think you mean coir verm gypsum which is what im using

And if it is my spawn that fucking sucks cause Im uber careful and started pcing for 2 hrs to ensure I dont get contams

I thought I got the 180 for two hours off this site *shrug* guess That needs to change


Edited by Kalypto (10/22/13 04:37 PM)


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OfflineStromriderM
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014547 - 10/22/13 04:42 PM (10 years, 4 months ago)

I agree 180 is too high. I can also say I rarely see any pins in 5 days. It usually more like 10. Coir/verm/gypsum is pretty forgiving even if you sterilize it.

May have just been dirty spawn. Even if you pc your jars properly you can still get a contam during inoculation. What did you use to inoculate your jars?


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InvisibleThadeous
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014551 - 10/22/13 04:42 PM (10 years, 4 months ago)

Yeah the sites information is dated at best. 

When you find time, check out the link I posted up above; it is a bit of work, but well worth it.  :sporedrop:


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OfflineKalypto
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Thadeous]
    #19014579 - 10/22/13 04:47 PM (10 years, 4 months ago)

Quote:

Stromrider said:
I agree 180 is too high. I can also say I rarely see any pins in 5 days. It usually more like 10. Coir/verm/gypsum is pretty forgiving even if you sterilize it.

May have just been dirty spawn. Even if you pc your jars properly you can still get a contam during inoculation. What did you use to inoculate your jars?




From prior experience I always get pins in under five days , but I did also wait a week to consolidate back then ( now outdated).

I use syringes still , which Im starting to think even the best ones have some contams

once my RM moves out Ill have a whole room as a lab so Ill be getting a LFH to do wedges

Quote:

Thadeous said:
Yeah the sites information is dated at best. 

When you find time, check out the link I posted up above; it is a bit of work, but well worth it.  :sporedrop:





Totally plan on using it tonight if possible

Well i think im gonna try his timings


Edited by Kalypto (10/22/13 04:49 PM)


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OfflineStromriderM
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Thadeous]
    #19014583 - 10/22/13 04:48 PM (10 years, 4 months ago)

Thad it's good to see you back active on the forum. You haven't really been around much since I joined but I have read many of your old post and learned a lot from them. Hope you stick around some


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OfflineKalypto
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Stromrider]
    #19014606 - 10/22/13 04:52 PM (10 years, 4 months ago)

Totally I only stopped growing cause I was moving , Ive still got a TON of learning to do , I one day HOPE to have my own strain


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InvisibleGretchenmeister
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014699 - 10/22/13 05:11 PM (10 years, 4 months ago)

Yeah I might not have messed with them so much, can take a week or more to see pins.  I notice your tubs are on the ground... in general it is recommended you keep your projects at least three feet above grounds surface, as the nastiest of contams float around in this region usually.


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OfflineKizzle
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Re: Problems : Tubs wont pin , Contam starting to take over [Re: Kalypto]
    #19014727 - 10/22/13 05:15 PM (10 years, 4 months ago)

Quote:

From prior experience I always get pins in under five days , but I did also wait a week to consolidate back then ( now outdated).



Consolidating's not outdated, it's just optional. Like the others I wouldn't be too worried after only 4-5 days, they look alright.


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