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Offlineillumi.naughty87
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Accidentally sucked air into syringe
    #19014145 - 10/22/13 03:11 PM (10 years, 3 months ago)

Title says it all. A MS syringe sucked 1-2 mL worth of air. I may just throw it out but I'm thinking about salvaging and innoc a jar of WBS to see if it contams. Any thoughts opinions or experience in this type of accident.


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OfflineStromriderM
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Re: Accidentally sucked air into syringe [Re: illumi.naughty87]
    #19014156 - 10/22/13 03:13 PM (10 years, 3 months ago)

Agar is your best bet.

If you don't do agar I guess you could noc up a grain jar and see what happens.


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Offlineillumi.naughty87
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Re: Accidentally sucked air into syringe [Re: Stromrider]
    #19014176 - 10/22/13 03:18 PM (10 years, 3 months ago)

I still haven't worked with agar. I recently (finally) got a PC and I am finally branching out away from BRF jars. I'm one step at a time and agar is next up.


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Invisiblespaceman101
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Re: Accidentally sucked air into syringe [Re: illumi.naughty87]
    #19014424 - 10/22/13 04:07 PM (10 years, 3 months ago)

I don't really understand your situation but I suck air into my MS syringes every time right before I use them for inoculation to mix the spores around better or to break up a chunk if their all bunched together and I have never had a contam until I open the jars or start fruiting and I don't get them very often so your good if it's in that context.


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Offlineillumi.naughty87
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Re: Accidentally sucked air into syringe [Re: spaceman101]
    #19014485 - 10/22/13 04:24 PM (10 years, 3 months ago)

@ spaceman 101.... Do you suck up straight up air? Or like the needle in an open flame trick?


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OfflineOregonMushys
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Re: Accidentally sucked air into syringe [Re: illumi.naughty87]
    #19014528 - 10/22/13 04:38 PM (10 years, 3 months ago)

Flame sterilize the needle red hot, then, as its red hot, make sure the entire open end of the needle is inside the flame, and suck in a bit of air while in the flame. Never suck in normal air thats literally inviting contams for dinner, and a couple cc's? Damn.

Ive never done the suck air through the flame trick, but ive read about it. :shrug:

If i were you i would just inoc some jars as usual, see how they go, if you get some fully colonized without contams and fruited, take prints, and start agar. Inoc a drop onto agar with this syringe you just goofd on :lol: because if there are any contams you can isolate away from them.


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Offlineillumi.naughty87
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Re: Accidentally sucked air into syringe [Re: OregonMushys]
    #19014742 - 10/22/13 05:18 PM (10 years, 3 months ago)

I know how to sterilize the needle and all. This was pure accidental isolated incident on my part. I just knocked up 3 WBS jars with the syringe in question, wish me luck on those. As for agar, I put the syringe in the refrigerator to wait for agar to show up. I will order some next week. I can't wait to play in the agar.


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Invisiblespaceman101
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Re: Accidentally sucked air into syringe [Re: illumi.naughty87]
    #19014836 - 10/22/13 05:32 PM (10 years, 3 months ago)

well what I do is when I get a new syringe it has a cap over it before you put the needle on so what I do is loosen the cap and suck the air around 1 or 2 cc's before I take the cap off and like I've said no problems at all.


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Offlineillumi.naughty87
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Re: Accidentally sucked air into syringe [Re: spaceman101]
    #19014974 - 10/22/13 05:54 PM (10 years, 3 months ago)

That's good to hear. Hearing that you do that and has always been contam free makes me feel like that syringe of mine has a chance. Thanks everyone!


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