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illumi.naughty87
Old Uncle Nutty



Registered: 03/05/13
Posts: 296
Last seen: 4 years, 11 months
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Accidentally sucked air into syringe
#19014145 - 10/22/13 03:11 PM (10 years, 3 months ago) |
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Title says it all. A MS syringe sucked 1-2 mL worth of air. I may just throw it out but I'm thinking about salvaging and innoc a jar of WBS to see if it contams. Any thoughts opinions or experience in this type of accident.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 22 minutes, 39 seconds
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Agar is your best bet.
If you don't do agar I guess you could noc up a grain jar and see what happens.
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illumi.naughty87
Old Uncle Nutty



Registered: 03/05/13
Posts: 296
Last seen: 4 years, 11 months
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Re: Accidentally sucked air into syringe [Re: Stromrider]
#19014176 - 10/22/13 03:18 PM (10 years, 3 months ago) |
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I still haven't worked with agar. I recently (finally) got a PC and I am finally branching out away from BRF jars. I'm one step at a time and agar is next up.
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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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I don't really understand your situation but I suck air into my MS syringes every time right before I use them for inoculation to mix the spores around better or to break up a chunk if their all bunched together and I have never had a contam until I open the jars or start fruiting and I don't get them very often so your good if it's in that context.
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illumi.naughty87
Old Uncle Nutty



Registered: 03/05/13
Posts: 296
Last seen: 4 years, 11 months
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Re: Accidentally sucked air into syringe [Re: spaceman101]
#19014485 - 10/22/13 04:24 PM (10 years, 3 months ago) |
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@ spaceman 101.... Do you suck up straight up air? Or like the needle in an open flame trick?
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OregonMushys
Rye Wata Whippin



Registered: 09/09/13
Posts: 280
Last seen: 6 years, 4 months
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Flame sterilize the needle red hot, then, as its red hot, make sure the entire open end of the needle is inside the flame, and suck in a bit of air while in the flame. Never suck in normal air thats literally inviting contams for dinner, and a couple cc's? Damn.
Ive never done the suck air through the flame trick, but ive read about it. 
If i were you i would just inoc some jars as usual, see how they go, if you get some fully colonized without contams and fruited, take prints, and start agar. Inoc a drop onto agar with this syringe you just goofd on because if there are any contams you can isolate away from them.
-------------------- Ps. Cubensis Ps. Cyanescens Ps. Stuntzii *GrowLog*
    
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illumi.naughty87
Old Uncle Nutty



Registered: 03/05/13
Posts: 296
Last seen: 4 years, 11 months
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Re: Accidentally sucked air into syringe [Re: OregonMushys]
#19014742 - 10/22/13 05:18 PM (10 years, 3 months ago) |
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I know how to sterilize the needle and all. This was pure accidental isolated incident on my part. I just knocked up 3 WBS jars with the syringe in question, wish me luck on those. As for agar, I put the syringe in the refrigerator to wait for agar to show up. I will order some next week. I can't wait to play in the agar.
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spaceman101
Friend to all



Registered: 01/18/13
Posts: 11,726
Loc: In heaven bored as hell
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well what I do is when I get a new syringe it has a cap over it before you put the needle on so what I do is loosen the cap and suck the air around 1 or 2 cc's before I take the cap off and like I've said no problems at all.
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illumi.naughty87
Old Uncle Nutty



Registered: 03/05/13
Posts: 296
Last seen: 4 years, 11 months
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Re: Accidentally sucked air into syringe [Re: spaceman101]
#19014974 - 10/22/13 05:54 PM (10 years, 3 months ago) |
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That's good to hear. Hearing that you do that and has always been contam free makes me feel like that syringe of mine has a chance. Thanks everyone!
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